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The detection of African horse sickness virus antigens and antibodies in young equidae

Published online by Cambridge University Press:  15 May 2009

C. Hamblin ,
E. C. Anderson ,
P. S. Mellor ,
S. D. Graham ,
P. P. C. Mertens  and
J. N. Burroughs
C. Hamblin
Affiliation:
Department of Virus Diagnosis, AFRC Institute for Animal Health, Pirbright Laboratory, Pirbright, Woking, Surrey GU24 0NF
E. C. Anderson
Affiliation:
Department of Virus Diagnosis, AFRC Institute for Animal Health, Pirbright Laboratory, Pirbright, Woking, Surrey GU24 0NF
P. S. Mellor
Affiliation:
Department of Virus Diagnosis, AFRC Institute for Animal Health, Pirbright Laboratory, Pirbright, Woking, Surrey GU24 0NF
S. D. Graham
Affiliation:
Department of Virus Diagnosis, AFRC Institute for Animal Health, Pirbright Laboratory, Pirbright, Woking, Surrey GU24 0NF
P. P. C. Mertens
Affiliation:
Department of Virus Diagnosis, AFRC Institute for Animal Health, Pirbright Laboratory, Pirbright, Woking, Surrey GU24 0NF
J. N. Burroughs
Affiliation:
Department of Virus Diagnosis, AFRC Institute for Animal Health, Pirbright Laboratory, Pirbright, Woking, Surrey GU24 0NF
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Four ponies were each inoculated with a different serotype of African horse sickness virus (AHSV) which had been passaged through cell culture in order to achieve attenuation. Three of the ponies died suddenly after showing mild clinical signs, the fourth pony remained clinically normal and was killed at day 38. Infectious AHSV was isolated from blood samples collected at intervals from all four ponies. Positive antigen ELISA reactions were only observed with blood samples from two of the ponies on the two days preceding death. Specific AHSV antibodies were detected by ELISA in serum samples from the other two ponies although one eventually died. African horse sickness viral antigens were detected by ELISA in post-mortem tissue samples collected from all four ponies. No infectious virus could be detected in tissue samples taken post-mortem from the pony which survived African horse sickness (AHS) infection. In the event of a suspected outbreak of AHS it is recommended that sera and heparinized blood should be tested for specific antibodies and AHSV antigen respectively. When available, post-mortem tissues, including spleen, heart, lung and liver, should also be tested for AHSV antigen. Although the ELISA used for the detection of AHSV antigen is highly sensitive and specific, negative ELISA results should be confirmed by virus isolation attempts.

Type
Research Article
Information
Epidemiology & Infection , Volume 108 , Issue 1 , February 1992 , pp. 193 - 201
Copyright
Copyright © Cambridge University Press 1992

References

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