Hostname: page-component-848d4c4894-2pzkn Total loading time: 0 Render date: 2024-05-20T18:04:35.340Z Has data issue: false hasContentIssue false
  • English
  • Français

The Demonstration of the Increase of Globulin in Diphtheria Antitoxins by the Precipitation Reaction

Published online by Cambridge University Press:  15 May 2009

G. L. Taylor
G. L. Taylor
Affiliation:
John Lucas Walker Student, From the Department of Pathology, University of Cambridge
Rights & Permissions [Opens in a new window]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. By titration against horse serum and its protein fractions anti-horse sera can be divided into three groups, according to the antibodies responsible for the main or earliest particulating zone, namely the sera containing antibodies against (a) globulin only, (b) albumin only, and (c) both globulin and albumin.

2. For the quantitative estimation of a protein fraction in a horse serum it is necessary to use an antiserum containing a main zone antibody acting with that protein only.

This is illustrated in experiments, designed to show the increased globulin content of diphtheria antitoxin, in which antisera containing antibodies against both globulin and albumin failed to register an increase in the globulin content.

Type
Research Article
Information
Journal of Hygiene , Volume 35 , Issue 2 , May 1935 , pp. 174 - 179
Copyright
Copyright © Cambridge University Press 1935

References

REFERENCES

Adair, G. S. and Robinson, Muriel E. (1930). The specific refraction increments of serum-albumin and serum-globulin. Biochem. J. 24, 993–1011.Google Scholar
Dean, H. R. (1931). The precipitation reaction. Med. Reg. Council, System of Bacteriology, 6, 424–51. London.Google Scholar
Dean, H. R. and Webb, R. A. (1926). The influence of optimal proportions of antigen and antibody in the serum precipitation reaction. J. Path. and Bact. 29, 473–92.CrossRefGoogle Scholar
Gibson, R. B. and Banzhaf, E. J. (1910). The quantitative changes in the proteins in the blood plasma of horses in the course of immunization. J. Exp. Med. 12, 411–34.Google Scholar
Goldsworthy, N. E. and Rudd, G. V. (1935). Complexity of antigens in relation to zones in the precipitation reaction. J. Path. and Bact. 40, 169–85.Google Scholar
Hiss, P. H. Jr. and Atkinson, J.P. (1900). Serum-globulin and diphtheric antitoxin. A comparative study of the amount of globulin in normal and antitoxic sera, and the relation of the globulin to the antitoxic bodies. J. Exp. Med. 5, 47–66.Google Scholar
Ledingham, J. C. G. (1907). On the relation of the antitoxin to the globulin-content of the blood serum during diphtheria immunisation. J. Hygiene, 7, 65–91.Google Scholar
Taylor, G. L. (1931). The results of some quantitative experiments on the serum precipitation reaction. Ibid. 31, 56–83.CrossRefGoogle Scholar
Taylor, G. L., Adair, G. S. and Adair, Muriel E. (1932). The estimation of proteins by the precipitation reaction. Ibid. 32, 340–8.CrossRefGoogle Scholar