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Criteria for measuring the efficacy of trachoma vaccines in baboons

Published online by Cambridge University Press:  15 May 2009

L. H. Collier  and
Elaine Lightman
L. H. Collier
Affiliation:
Medical Research Council Trachoma Unit, Lister Institute of Preventive Medicine, London, S. W. 1
Elaine Lightman
Affiliation:
Medical Research Council Statistical Research and Services Unit, University College Hospital Medical School, London, W.C. 1
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Trachoma vaccines are usually assayed by testing their ability to protect monkeys or baboons against subsequent challenge of the conjunctiva with a pathogenic strain of trachoma/inclusion conjunctivitis (TRIC) agent. In such experiments the course of infection in vaccinated baboons was compared in terms of arbitrary scores assigned to a range of clinical signs, and of counts of TRIC inclusions in conjunctival scrapings. Analysis of many such scores indicated that after a large challenge dose of strain MRC-4s, the scores for signs of inflammation reached their maximum earlier than the follicle score; the inflammation score was closely related to the number of inclusions, whereas the follicle score was not. With this system, the optimum periods for eliciting differences between vaccinated and control measures varied according to the sign used; it was later for follicles than for inflammation or inclusions. For assessing the influence of vaccination, the mean of the inflammation scores read weekly for the first 3 weeks after challenge and the mean inclusion score over the same period were equally satisfactory, and either was rather better than the mean of three follicle scores taken over the period 3–6 weeks.

For assessing the influence of vaccines or therapeutic agents on experimental trachoma it is important to determine which signs discriminate best between treated and control animals, and the optimum times for measuring them.

Type
Research Article
Information
Journal of Hygiene , Volume 69 , Issue 4 , December 1971 , pp. 717 - 725
Copyright
Copyright © Cambridge University Press 1971

References

REFERENCES

Assaad, F. A. & Maxwell-Lyons, F. (1967). Application of clinical scoring systems to trachoma research. American Journal of Ophthalmology 63, 1327.CrossRefGoogle ScholarPubMed
Collier, L. H. (1961). Experiments with trachoma vaccines: experimental system using inclusion blennorrhoea virus. Lancet i, 795.Google Scholar
Collier, L. H. (1967). The immunopathology of trachoma: some facts and fancies. Archiv für die gesamte Virusforschung 22, 280.CrossRefGoogle ScholarPubMed
Collier, L. H. & Blyth, W. A. (1966 a). Immunogenicity of experimental trachoma vaccines in baboons. I. Experimental methods, and preliminary tests with vaccines prepared in chick embryos and in HeLa cells. Journal of Hygiene 64, 513.Google Scholar
Collier, L. H. & Blyth, W. A. (1966 b). Immunogenicity of experimental trachoma vaccines in baboons. II. Experiments with adjuvants, and tests of cross-protection. Journal of Hygiene 64, 529.Google ScholarPubMed
Collier, L. H. & Smith, A. (1967). Dissemination and irnmunogenicity of live TRIC agent in baboons after parenteral injection. American Journal of Ophthalmology 63, 1589.CrossRefGoogle ScholarPubMed
Dawson, C., Jawetz, E., Thygeson, P. & Hanna, L. (1961). Trachoma viruses isolated in the United States. 4. Infectivity and immunogenicity for monkeys. Proceedings of the Society for Experimental Biology and Medicine 106, 898.CrossRefGoogle ScholarPubMed
Dawson, C. R., Mordhorst, C. H. & Thygeson, P. (1962). Infection of rhesus and cynomolgus monkeys with egg-grown viruses of trachoma and inclusion conjunctivitis. Annals of the New York Academy of Sciences 98, 167.CrossRefGoogle ScholarPubMed
Gear, J. H. S., Gordon, P. B., Jones, B. R. & Bell, S. D. (1963). Nomenclature of isolates of virus from trachoma and inclusion blennorrhoea. Nature, London 197, 26.Google Scholar
Grayston, J. T., Wang, S. P., Woolridge, R. L., Yang, Y. F. & Johnston, P. B. (1960). Trachoma: studies of etiology, laboratory diagnosis, and prevention. Journal of the American Medical Association 172, 1577.Google ScholarPubMed
Mordhorst, C. H. (1967). Experimental infections and immunogenicity of TRIC agents in monkeys. American Journal of Ophthalmology 63, 1603.Google Scholar
Reeve, P. & Taverne, J. (1963). Observations on the growth of trachoma and inclusion blennorrhoea viruses in embryonate eggs. Journal of Hygiene 61, 67.CrossRefGoogle Scholar
Reeve, P. & Taverne, J. (1967). The significance of strain differences in the behaviour of TRIC agents in the chick embryo. American Journal of Ophthalmology 63, 1162.CrossRefGoogle ScholarPubMed
Wang, S. P. (1967). Clinical evaluation of monkey infection with TRIC agents: a numerical scoring system of disease severity. American Journal of Ophthalmology 63, 1321.CrossRefGoogle ScholarPubMed
World Health Organization (1962). Expert Committee on Trachoma. Third Report. Technical Report Series, World Health Organization, no. 234.Google Scholar
World Health Organization (1966). Fourth Scientific Group on Trachoma Research. Technical Report Series, World Health Organization, no. 330.Google Scholar