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A comparison of typing methods for Serratia marcescens

Published online by Cambridge University Press:  15 May 2009

B. Anderhub ,
T. L. Pitt ,
Y. J. Erdman  and
W. R. Willcox
B. Anderhub
Affiliation:
Cross-Infection Reference Laboratory
T. L. Pitt
Affiliation:
Cross-Infection Reference Laboratory
Y. J. Erdman
Affiliation:
Cross-Infection Reference Laboratory
W. R. Willcox
Affiliation:
National Collection of Type Cultures Central Public Health Laboratory, Colindale Avenue, London NW9 5HT
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Summary

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A simple method for the bacteriocine typing of Serratia marcescens without the use of induction was sought. The results of a mutual inhibition experiment with 89 unrelated cultures indicated that a bacteriocine-susceptibility method would give more discrimination between strains than would a bacteriocine-production method. A cross-streaking technique for bacteriocine-susceptibility typing without previous induction was developed, and its performance was compared with that of another susceptibility-typing method in which cell-free lysates of the producer strains were obtained by induction with mitomycin C.

Replicate typing of the same collection of cultures by both methods indicated that small variations in pattern were common and that larger variations occurred occasionally. Differences in pattern of less than two strong reactions in the mito-mycin-C induction method, and of less than three strong reactions in the cross-streaking method, should therefore not be taken as evidence that strains can be distinguished.

Sets of cultures of Ser. marcescens, 178 in total, from a number of supposed incidents of infection in hospitals, were used to evaluate the two bacteriocine-typing methods; all of the cultures were also O serogrouped. Comparison of the typing patterns of members of the same O serogroup from clear-cut incidents of infection confirmed that results of acceptable reliability could be obtained by either bacteriocine-typing method by the application of the appropriate 'difference' rule. When so interpreted, the cross-streaking method appeared to be slightly the more discriminatory.

The greatest discrimination between strains was obtained by the use of a 'hierarchical' typing system in which the strains were first O serogrouped, and the cross-streaking method of bacteriocine typing was then used to make subdivisions within O serogroups.

Type
Research Article
Information
Journal of Hygiene , Volume 79 , Issue 1 , August 1977 , pp. 89 - 102
Copyright
Copyright © Cambridge University Press 1977

References

REFERENCES

Abbott, J. D. & Shannon, R. (1958). A method for typing Shigella sonnei using colicine production as a marker. Journal of Clinical Pathology 11, 71.CrossRefGoogle ScholarPubMed
Altemeier, W. A., Culbertson, W. R., Fullen, W. D. & McDonough, J. J. (1969). Serratia marcescens septicemia: a new threat in surgery. Archives of Surgery 99, 232.CrossRefGoogle ScholarPubMed
Asheshov, E. H. (1974). An assessment of the methods used for typing strains of Pseudomonas aeruginosa. In Proceedings of 6th National Congress of Bacteriology, edited by Arseni, A., Athens, p. 9.Google Scholar
Baumann, W. & Emmrich, P. (1974). Sepsis und andere Infektionen durch Serratia marcescens im Neugeborenen-und Säuglingsalter. Deutsche Medizinische Wochenschrift 99, 1755.CrossRefGoogle Scholar
Blair, J. E. & Williams, R. E. O. (1961). Phage typing of staphylococci. Bulletin of the World Health Organization 24, 771.Google ScholarPubMed
Cradock-Watson, J. E. (1965). The production of bacteriocines by Proteus species. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene, Abt. 1, Orig. 196, 385.Google Scholar
Daschner, F. & Senska-Euringer, C. (1975). Kontaminierte Infusionen als Ursache nosokomialer Serratia-marcescens-sepsis bei Kindern. Deutsche Medizinische Wochenschrift 100, 2324.CrossRefGoogle Scholar
DuPont, H. L. & Spink, W. W. (1969). Infections due to Gram-negative organisms: an analysis of 860 patients with bacteremia at the University of Minnesota Medical Center, 1958–1966. Medicine 48, 307.CrossRefGoogle ScholarPubMed
Edwards, P. R. & Ewing, W. H. (1962). In Identification of Enterobacteriaceae. 2nd edition, p. 223. Minneapolis, Minnesota: Burgess Publishing Company.Google Scholar
Ewing, W. H., Davis, B. R. & Reavis, R. W. (1959). Studies on the Serratia group. CDC Laboratory Manual. Center for Disease Control, Atlanta, Georgia, U.S.A.Google Scholar
Ewing, W. H., Johnson, J. G. & Davis, B. R. (1962). The occurrence of Serratia marcescens in nosocomial infections. CDC Publication. Center for Disease Control, Atlanta, Georgia, U.S.A.Google Scholar
Farmer, J. J. (1972). Epidemiological differentiation of Serratia marcescens: typing by bacteriocin production. Applied Microbiology 23, 218.CrossRefGoogle ScholarPubMed
Farmer, J. J. & Herman, L. G. (1969). Epidemiological fingerprinting of Pseudomonas aeruginosa by the production of and sensitivity to pyocin and bacteriophage. Applied Microbiology 18, 760.CrossRefGoogle ScholarPubMed
Gillies, R. R. & Govan, J. R. W. (1966). Typing of Pseudomonas pyocyanea by pyocine production. Journal of Pathology and Bacteriology 91, 339.CrossRefGoogle ScholarPubMed
Jones, L. F., Thomas, E. T., Stinnett, J. D., Gilardi, G. L. & Farmer, J. J. (1974). Pyocin sensitivity of Pseudomonas species. Applied Microbiology 27, 288.CrossRefGoogle ScholarPubMed
Meitert, T. & Meitert, E. (1966). Utilisation combinée du sérotype et de la lysotypie des souches de Pseudomonas aeruginosa en vue d'approfondir les investigations épidemiologiques. Archives Roumaines de Pathologie Experimentale et de Microbiologie 25, 427.Google Scholar
Pillich, J., Hradečná, Z. & Kocur, M. (1964). An attempt at phage typing in the genus Serratia. Journal of Applied Bacteriology 27, 65.CrossRefGoogle Scholar
Prinsloo, H. E. (1966). Bacteriocins and phages produced by Serratia marcescens. Journal of General Microbiology 45. 205.CrossRefGoogle ScholarPubMed
Rampling, A., Whitby, J. L. & Wildy, P. (1975). Pyocin sensitivity testing as a method of typing Pseudomonas aeruginosa: use of 'phage-free' preparations of pyocin. Journal of Medical Microbiology 8, 531.CrossRefGoogle ScholarPubMed
Traub, W. H., Raymond, E. A. & Startsman, T. S. (1971). Bacteriocin (marcescin) typing of clinical isolates of Serratia marcescens. Applied Microbiology 21, 837.CrossRefGoogle ScholarPubMed
Wahba, A. H. (1963). The production and inactivation of pyocines. Journal of Hygiene 61, 431.Google ScholarPubMed
Wilfert, J. N., Barrett, F. F. & Kass, E. H. (1968). Bacteremia due to Serratia marcescens. New England Journal of Medicine 279, 286.CrossRefGoogle ScholarPubMed
Wilfert, J. N., Barrett, F. F., Ewing, W. H., Finland, M. & Kass, E. H. (1970). Serratia marcescens: biochemical, serological and epidemiological characteristics, and antibiotic susceptibility of strains isolated at Boston City Hospital. Applied Microbiology 19, 345.CrossRefGoogle ScholarPubMed
Wilkowske, C. J., Washington, J. A., Martin, W. J. & Ritts, R. E. J. (1970). Serratia marcescens: biochemical characteristics, antibiotic susceptibility patterns, and clinical significance. Journal of the American Medical Association 214, 2157.CrossRefGoogle ScholarPubMed
Willcox, W. R. & Lapage, S. P. (1972). Automatic construction of diagnostic tables. The Computer Journal 15, 263.CrossRefGoogle Scholar
Williams, R. E. O. & Rippon, J. E. (1952). Bacteriophage typing of Staphylococcus aureus. Journal of Hygiene 50, 320.CrossRefGoogle ScholarPubMed