Hostname: page-component-77c89778f8-swr86 Total loading time: 0 Render date: 2024-07-20T06:56:32.800Z Has data issue: false hasContentIssue false
  • English
  • Français

Comparison of agglutination, complement fixation and immunofluorescence tests in Campylobacter jejuni infections

Published online by Cambridge University Press:  25 March 2010

K. C. Watson  and
E. J. C. Kerr
K. C. Watson
Affiliation:
Central Microbiological Laboratories, Western General Hospital, Edinburgh EH4 2XU
E. J. C. Kerr
Affiliation:
Central Microbiological Laboratories, Western General Hospital, Edinburgh EH4 2XU
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Good antibody responses usually follow infection with Campylobacter jejuni. A comparison of agglutination, complement fixation and immunofluorescence tests was done on 55 sera from 40 sporadic patients with diarrhoeal disease and positive cultures for C. jejuni. Results showed 82 % positive with immunofluorescence, 62 % by complement fixation but only 38 % by agglutination, using two reference strains COP and MEL as antigens. Overall 90 % of the 40 patients were positive by one or more serological tests. Paired sera from 15 patients showed a fourfold or greater rise in only five, confirming previous observations that antibody formation occurs early in the course of infection. Results also suggest that different test systems may be detecting antibodies of different specificities. Results confirm the value of serological tests, but further information on serotypes is required for selection of suitable reference strains.

Type
Research Article
Information
Journal of Hygiene , Volume 88 , Issue 2 , April 1982 , pp. 165 - 171
Copyright
Copyright © Cambridge University Press 1982

References

REFERENCES

Abbott, J. D., Dale, B., Eldridge, J., Jones, D. M. & Sutcliffe, E. M. (1980). Serotyping of Campylobacter jejuni/coli. Journal of Clincal Pathology 33, 762–766.CrossRefGoogle ScholarPubMed
Jones, D. M. (1981). Personal communication.Google Scholar
Jones, D. M., Eldridge, J. & Dale, B. (1980). Serological response to Campylobacter jejuni/coli infection. Journal of Clinical Pathology 33, 767–769.CrossRefGoogle ScholarPubMed
Piemant, Y. & Abanamy, A. (1980). Campylobacter jejuni agent de diarrhées: expérience d'un an de recherches systématiques. Médicine et Maladies Infectieuses 10, 994–1300.Google Scholar
Skirrow, M. B. (1977). Campylobacter enteritis: A ‘New’ disease. British Medical Journal ii, 9–11.CrossRefGoogle Scholar
Watson, K. C., Kerr, E. J. C. & Jack, I. (1981). Comparison of agglutination and complement fixation tests in diagnosis of human Campylobacter infections. Journal of Infection. (In the Press.)Google Scholar
Watson, K. C., Kerr, E. J. C. & McFadzean, S. (1979). Serology of human Campylobacter infections. Journal of Infection 1, 151–158.CrossRefGoogle Scholar