Hostname: page-component-8448b6f56d-cfpbc Total loading time: 0 Render date: 2024-04-18T05:55:52.663Z Has data issue: false hasContentIssue false
  • English
  • Français

Clonal diffusion of EPEC-like Escherichia coli from rabbits as detected by ribotyping and random amplified polymorphic DNA assays

Published online by Cambridge University Press:  15 May 2009

S. Leroy-Setrin ,
M. C. Lesage ,
E. Chaslus-Dancla  and
J. P. Lafont
S. Leroy-Setrin
Affiliation:
Station de Pathologie Aviaire et de Parasitologie, Institut National de la Recherche Aqronomique, Centre de Tours, 37380 Nouzilly, France
M. C. Lesage
Affiliation:
Station de Pathologie Aviaire et de Parasitologie, Institut National de la Recherche Aqronomique, Centre de Tours, 37380 Nouzilly, France
E. Chaslus-Dancla*
Affiliation:
Station de Pathologie Aviaire et de Parasitologie, Institut National de la Recherche Aqronomique, Centre de Tours, 37380 Nouzilly, France
J. P. Lafont
Affiliation:
Station de Pathologie Aviaire et de Parasitologie, Institut National de la Recherche Aqronomique, Centre de Tours, 37380 Nouzilly, France
*
* Author for correspondence.
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The genetic diversity and clonal relationships among 77 Escherichia coli strains isolated in France from diarrhoeic rabbits and that belonged to seven O serogroups including the predominant O103 serogroup, were estimated by ribotyping and random amplified polymorphic DNA (RAPD) assays. Fifteen ribotypes were defined. Most of the highly pathogenic O103 strains could be assigned to two major groups. Non-pathogenic strains were clearly distinguished. RAPD assays generally matched ribotyping, or gave more precision for subdividing strains from the two main O103 groups. The results on strains isolated from different areas and over a 9-year period showed the relevance of the association of these two methods for the survey of the spread of strains in breeding flocks and illustrated clonal diffusion in rabbit production structures.

Type
Special Article
Information
Epidemiology & Infection , Volume 114 , Issue 1 , February 1995 , pp. 113 - 121
Copyright
Copyright © Cambridge University Press 1995

References

REFERENCES

1.Camguilhem, R, Milon, A. Biotypes and O serogroups of Escherichia coli involved in intestinal infections of weaned rabbits: clues to diagnosis of pathogenic strains. J Clin Microbiol 1989; 27: 743–7.CrossRefGoogle Scholar
2.Peeters, JE, Geeroms, R, Orskov, F. Biotype, serotype, and pathogenicity of attaching and effacing enteropathogenic Escherichia coli strains isolated from diarrheic commercial rabbits. Infect Immun 1988; 56: 1442–8.CrossRefGoogle ScholarPubMed
3.Cantey, JR, Blake, RK. Diarrhea due to Escherichia coli in the rabbit: a novel mechanism. J Infect Dis 1977; 135: 454–62.Google Scholar
4.Jerse, AE, Yu, J, Tall, BD, Kaper, JB. A genetic locus of enteropathogenic Escherichia coli necessary for the production of attaching and effacing lesions on tissue culture cells. Proc Natl Acad Sci USA 1990; 87: 7839–43.CrossRefGoogle ScholarPubMed
5.Pohl, PH, Peeters, JE, Jacquemin, ER, Lintermans, PF, Mainil, JG. Identification of eae sequences in enteropathogenic Escherichia coli strains from rabbits. Infect Immun 1993; 61: 2203–6.Google Scholar
6.Leroy, SM, Lesage, MC, Chaslus-Dancla, E, Lafont, JP. Presence of eaeA sequences in pathogenic and non-pathogenic Escherichia coli strains isolated from weaned rabbits. J Med Microbiol 1994; 40: 90–4.CrossRefGoogle ScholarPubMed
7.Donnenberg, MS, Kaper, JB. Enteropathogenic Escherichia coli. Infect Immun 1992; 60: 3953–61.CrossRefGoogle ScholarPubMed
8.Grimont, F, Grimont, PDA. Ribosomal ribonucleic acid gene restriction patterns as potential taxonomic tools. Ann Inst Pasteur Microbiol 1986; 137B: 165–75.Google Scholar
9.Welsh, J, McClealland, M. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 1990; 18: 7213–18.Google Scholar
10.Williams, JGK, Kubelik, AR, Livak, KJ, Rafalski, JA, Tingey, SV. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 1990; 18: 6531–5.CrossRefGoogle ScholarPubMed
11.Milon, A, Esslinger, J, Camguilhem, R. Adhesion of Escherichia eoli strains isolated from diarrheic weaned rabbits to intestinal villi and Hela cells. Infect Immun 1990; 58: 2690–5.CrossRefGoogle Scholar
12.Mariani-Kurkdjian, P, Denamur, E, Milon, A et al. , Identification of a clone of Escherichia coli O103:H2 as a potential agent of hemolytic-uremic syndrome in France. J Clin Microbiol 1993; 31: 296–301.CrossRefGoogle Scholar
13.Wilson, K. Preparation of genomic DNA from bacteria. In: Ausubel, FM, Brent, R, Kingston, RE, Moore, DD, Smith, JA, Seidman, JG, Struhl, K, eds. Current protocols in molecular biology, vol. 1. New York: Wiley, 1987.Google Scholar
14.Sambrook, J, Fritsch, EF, Maniatis, T. Molecular cloning – a laboratory manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press, 1989.Google Scholar
15.Picard-Pasquier, N, Ouagued, M, Picard, B, Goullet, P, Krishnamoorthy, R. A simple, sensitive method of analysing bacterial ribosomal DNA polymorphism. Electrophoresis 1989; 10: 186–9.CrossRefGoogle Scholar
16.Meunier, JR, Grimont, PAD. Factors affecting reproducibility of random amplified polymorphic DNA fingerprinting. Res Microbiol 1993; 144: 373–9.CrossRefGoogle ScholarPubMed