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Circulating antibodies against faecal bacteria assessed by immunomorphometry: combining quantitative immunofluorescence and image analysis

Published online by Cambridge University Press:  15 May 2009

H. Z. Apperloo-Renkema ,
M. H. F. Wilkinson  and
D. van der Waaij
H. Z. Apperloo-Renkema
Affiliation:
Laboratory for Medical Microbiology, State University Groningen, Oostersingel 59, 9713 EZ Grongingen, The Netherlands
M. H. F. Wilkinson
Affiliation:
Laboratory for Medical Microbiology, State University Groningen, Oostersingel 59, 9713 EZ Grongingen, The Netherlands
D. van der Waaij
Affiliation:
Laboratory for Medical Microbiology, State University Groningen, Oostersingel 59, 9713 EZ Grongingen, The Netherlands
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Summary

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A new technique to study the prevalence of circulating antibodies directed against different morphological groups (‘morphotypes’) of bacteria in fresh faeces is presented. The technique combines quantitative indirect immunofluorescence with digital image analysis. Plasma antibody titres and patterns of IgA, IgG and IgM isotype against morphotypes of faecal bacteria were determined in ten healthy individuals.

Type
Research Article
Information
Epidemiology & Infection , Volume 109 , Issue 3 , December 1992 , pp. 497 - 506
Copyright
Copyright © Cambridge University Press 1992

References

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