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Chemical disinfection of human rotavirus-contaminated inanimate surfaces

Published online by Cambridge University Press:  19 October 2009

Nellie Lloyd-Evans ,
V. Susan Springthorpe  and
Syed A. Sattar
Nellie Lloyd-Evans
Affiliation:
Department of Microbiology and Immunology, School of Medicine, University of Ottawa, Ottawa, Ontario, CanadaK1H 8M5
V. Susan Springthorpe
Affiliation:
Department of Microbiology and Immunology, School of Medicine, University of Ottawa, Ottawa, Ontario, CanadaK1H 8M5
Syed A. Sattar*
Affiliation:
Department of Microbiology and Immunology, School of Medicine, University of Ottawa, Ottawa, Ontario, CanadaK1H 8M5
*
*Author for correspondence.
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Fomites may play a role in the transmission of rotavirus infections, and in view of this, 27 disinfectants were evaluated for their ability to inactivate human rotavirus (HRV) on contaminated non-porous inanimate surfaces. Disks of stainless steel, glass and two types of plastics were contaminated with about 107 plaque-forming units of HRV suspended in faecal matter. The inoculum was allowed todry and an equal volume of the product under test was applied to the contaminated surface. After contact for 1 min, the action of the disinfectant was stopped by dilution. Surviving infectious virus on the disks was determined by plaque assay in MA-104 cells. A product was considered to be effective if itcould reduce the virus titre by at least 3 log10. Only 33·3% (9/27) of the formulations tested proved to be effective. Further testing of the effective products, which included antiseptics, instrument soaks and hard-surface disinfectants, showed that all of them could, in fact, reduce the virus titre on contaminated surfaces by at least 6 log10. These findings show the relative resistance of HRV to a widerange of chemical disinfectants in common use, and also emphasize the need for a more thorough evaluation of the virucidal potential of formulations regularly employed in attempts to prevent and control outbreaks of rotaviral diarrhoea.

Type
Research Article
Information
Journal of Hygiene , Volume 97 , Issue 1 , August 1986 , pp. 163 - 173
Copyright
Copyright © Cambridge University Press 1986

References

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