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Characterization of multidrug-resistance phenotypes and genotypes of Escherichia coli strains isolated from swine from an abattoir in Osaka, Japan

Published online by Cambridge University Press:  30 November 2004

YUKO KUMAI ,
YASUHIKO SUZUKI ,
YOSHINORI TANAKA ,
KENSUKE SHIMA ,
RUPAK K. BHADRA ,
SHINJI YAMASAKI ,
KOICHI KURODA  and
GINJI ENDO
YUKO KUMAI
Affiliation:
Department of Preventive Medicine and Environmental Health, Osaka City University Medical School, Osaka, Japan
YASUHIKO SUZUKI
Affiliation:
Department of Microbiology and Pathology, Faculty of Medicine, Tottori University, Tottori, Japan
YOSHINORI TANAKA
Affiliation:
Department of Microbiology and Pathology, Faculty of Medicine, Tottori University, Tottori, Japan
KENSUKE SHIMA
Affiliation:
Department of Veterinary Science, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Osaka, Japan
RUPAK K. BHADRA
Affiliation:
Department of Veterinary Science, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Osaka, Japan Infectious Diseases Group, Indian Institute of Chemical Biology, Kolkata, India
SHINJI YAMASAKI
Affiliation:
Department of Veterinary Science, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Osaka, Japan
KOICHI KURODA
Affiliation:
Department of Preventive Medicine and Environmental Health, Osaka City University Medical School, Osaka, Japan
GINJI ENDO
Affiliation:
Department of Preventive Medicine and Environmental Health, Osaka City University Medical School, Osaka, Japan
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Abstract

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A total of 455 highly tetracycline-resistant Escherichia coli strains were isolated from 84 healthy swine from abattoirs and it was found that 56·9, 43·1, 22·2, 15·4, 2·6 and 1·5% of strains were resistant to chloramphenicol, ampicillin, kanamycin, trimethoprim–sulphamethoxazole, ofloxacin and gentamicin respectively. Interestingly, E. coli strains isolated from certain finisher hog groups exhibited resistance against 2–7 antimicrobials, but strains isolated from multiparous sow groups in each herd were resistant to only 2–4 antimicrobial agents. When randomly selected 108 tetracycline-resistant isolates were tested for the presence of resistance genes, the following genes tet(A) (n=6), tet(B) (n=95), tet(D) (n=1) or both tet(A) and tet(B) (n=6) were found to be distributed among them. Furthermore, 52 isolates carried the integrase 1 gene and 24 strains gave five different PCR amplicon profiles using primers from the variable region of integron. Extensive nucleotide sequence analyses of these amplicons revealed the presence of dhfrI, dhfrXII, dfr17, aadA, aadA2, aadA5, aadA21, aacA4 and catB3 genes which code for different antibacterial resistance proteins.

Type
Research Article
Information
Epidemiology & Infection , Volume 133 , Issue 1 , February 2005 , pp. 59 - 70
Copyright
2004 Cambridge University Press