Hostname: page-component-848d4c4894-wg55d Total loading time: 0 Render date: 2024-05-01T06:45:28.962Z Has data issue: false hasContentIssue false
  • English
  • Français

A μ-capture ELISA for detecting Mycoplasma pneumoniae IgM: comparison with indirect immunofluorescence and indirect ELISA

Published online by Cambridge University Press:  19 October 2009

T. G. Wreghitt  and
Margaret Sillis
T. G. Wreghitt
Affiliation:
Clinical Microbiology and Public Health Laboratory Level 6, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2QW
Margaret Sillis
Affiliation:
PHLS Mycoplasma Reference Laboratory, Bowthorpe Road, Norwich NR2 3TX
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

A μ-capture ELISA was developed for detecting Mycoplasma pneumoniae specific IgM, and compared with an indirect immunofluorescent antibody (IFA) technique and an indirect ELISA. μ-capture ELISA and IFA compared well and were found to be the most sensitive assays. The IFA test can be completed in 2 h whilst the results of the μ-capture ELISA can be available in 24 h. Both tests are amenable to routine diagnostic use and have similar sensitivity. Indirect ELISA was found to be less sensitive and less specific, giving high assay values with several sera having undetectable M. pneumoniae CF antibody or CF antibody in low titre. Serum samples obtained from 11 patients at various times after M. pneumoniae infection showed maximum antibody levels within the first month by all assays, with a gradual fall in amount of IgM with time when assayed by μ-capture ELISA, a more gradual decline by IFA and hardly any decline with indirect ELISA. It was concluded that the indirect ELISA is unsuitable for the investigation of possible M. pneumoniae infection because the sustained high assay values with serum samples taken many months after infection, make interpretation of the test results very difficult.

Type
Research Article
Information
Journal of Hygiene , Volume 94 , Issue 2 , April 1985 , pp. 217 - 227
Copyright
Copyright © Cambridge University Press 1985

References

REFERENCES

Biberfield, G. & Sterner, G. (1971). Antibodies in bronchial secretions following natural infection with Mycoplasma pneumoniae. Acta Pathologica Microbiologica Scandinavica, B 79, 599–605.Google Scholar
Brunner, H., Schaeg, W., Bruck, U., Schummer, U., Sziegoleit, D. & Schiefer, H. G. (1978). Determination of IgG, IgM and IgA antibodies to Mycoplasma pneumoniae by an indirect Staphylococcal Radio-immunoassay. Medical Microbiology and Immunology 165, 29–41.Google Scholar
Chamberlain, P. & Saeed, A. A. (1983). A study of the specific IgM antibody response in Mycoplasma pneumoniae infection in Man. Journal of Hygiene 90, 207–211.Google Scholar
Duermeyer, W., Wielaard, F. & van der Veen, J. (1979). A new principle for the detection of specific IgM antibodies appled to an ELISA for Hepatitis A. Journal of Medical Virology 4, 25–32.Google Scholar
Dussaix, E., Slim, A. & Tournier, P. (1983). Comparison of enzyme-linked immuno-sorbent assay (ELISA) and complement fixation test for detection of Mycoplasma pneumoniae antibodies. Journal of Clinical Pathology 36, 228–232.Google Scholar
Finland, M., Peterson, O. L., Allen, H. E., Samper, B. A. & Barnes, M. W. (1945). Cold agglutinins: cold isohaemagglutinins in primary atypical pneumonia of unknown etiology with a note on the occurrence of haemolytic anaemia in three cases. Journal of Clinical Investigation 24, 458–461.CrossRefGoogle Scholar
Kurtz, J. B. & Malic, A. (1981). Rubella-specific IgM detected by an antibody capture assay/ELISA technique. Journal of Clinical Pathology 34, 1392–1395.Google Scholar
Leach, R. H. (1973). Further studies on classification of bovine strains of Mycoplasmatales with proposals for new species, Acholeplasma modicum and Mycoplasma alkalescens. Journal of General Microbiology 75, 135–153.Google Scholar
Lemcke, R. M., Marmion, B. P. & Plackett, P. (1967). Immunochemical analysis of Mycoplasma pneumoniae. Annals of the New York Academy of Sciences 143, 691–702.Google Scholar
Mardh, P. A., Ursing, B. & Lund, K. (1975). Persistent cerebellar symptoms after infection with Mycoplasma pneumoniae. Scandinavian Journal of Infectious Disease 7, 157–160.Google ScholarPubMed
Morgan-Capner, P., Tedder, R. S. & Mace, J. E. (1983). Rubella-specific IgM reactivity in sera from cases of infectious mononucleosis, Journal of Hygiene 90, 407–413.Google Scholar
Mortimer, P. P., Tedder, R. S., Hambling, M. H., Shafi, M. S., Burkhardt, F. & Schilt, U. (1981). Antibody capture radioimmunoassay for anti-rubella IgM. Journal of Hygiene 86, 139–153.CrossRefGoogle ScholarPubMed
Price, P. C. (1980). Direct radioimmunoassay for the detection of IgM antibodies against Mycoplasma pneumoniae. Journal of Immunological Methods 32, 261–273.Google Scholar
Raisanen, S. M., Suni, J. I. & Leinikki, P. O. (1980). Serological diagnosis of Mycoplasma pneumoniae infection by enzyme immunoassay. Journal of Clinical Pathology 33, 836–840.CrossRefGoogle ScholarPubMed
Rifkind, D., Chanock, R. M., Kravetz, H.Johnson, K. & Knight, V. (1962). Ear involvement (myringitis) and primary atypical pneumonia following inoculation of volunteers with Eaton agent. American Review of Respiratory Diseases 85, 479–489.Google Scholar
Sillis, M. & Andrews, B. (1978). A simple test for M. pneumoniae IgM. Zentralblatt Sur Bakteriologie, Parasitemkunde, Infektionskramkheiten unt Hygiene Erste Abteilung Originale. Reihe A Medizinische Mikrobiologie unt Parasitologie 241, 239–240.Google Scholar
Skaug, K., Eng., J., Orstavik, I. & Haug, K.W (1976). The diagnostic value of determination of IgM antibodies against Mycoplasma pneumoniae by the indirect immunofluorescent antibody test. Acta Pathalogica et Microbiologica Scandinavica, B 84, 170–176.Google Scholar
Smith, A. M. & Tedder, R. S. (1981). Development of an enzyme-linked immunosorbent assay (ELISA) for Hepatitis Be antigen and antibody. Journal of Virological Methods 3, 1–11.Google Scholar
Swift, P. G. F. & Stevens, D. W. (1978). Mucocutaneous reactions during Mycoplasma pneumoniae infection. Lancet i, 1104–1105.Google Scholar
van Griethuysen, A. J. A., de Graaf, R.van Druten, J. A. M.Heesen, F. W. Avan der Logt, J. T. M. & van Loon, A. M. (1984). Use of the enzyme-linked immunosorbent assay for the early diagnosis of Mycoplasma pneumoniae infection. European Journal of Clinical Microbiology 3, 116–121.CrossRefGoogle ScholarPubMed
Wreghitt, T. G. & Nagington, J. (1983). Variability in EIA plates used in ELISA tests for the detection of antibodies to Legionella pneumophila. Journal of Clinical Pathology 36, 238–239.Google Scholar
Wreghitt, T. G., Tedder, R. S., Nagington, J. & Ferns, R. B. (1984). Antibody assays for varicella-zoster virus: comparison of competitive enzyme-linked immunosorbent assay (ELISA), competitive radioimmunoassay (RIA), complement fixation and indirect immunofluorescence assays. Journal of Medical Virology 13, 361–370.Google Scholar