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A blastogenic test for foot-and-mouth disease

Published online by Cambridge University Press:  15 May 2009

R. C. Wardley ,
W. G. Chapman  and
A. J. M. Garland
R. C. Wardley
Affiliation:
Animal Virus Research Institute, Pirbright, Surrey
W. G. Chapman
Affiliation:
Animal Virus Research Institute, Pirbright, Surrey
A. J. M. Garland
Affiliation:
Animal Virus Research Institute, Pirbright, Surrey
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A blastogenic test to detect peripheral blood leukocytes specifically sensitized to foot-and-mouth disease virus antigen is described. The test is carried out in microtitre plates and optimum conditions were found by titration. These employed 7.5 × 105 cells/well and 20 complement fixing units of antigen. Peak [3H]thymidine incorporation was found to take place at 2–3 days.

Type
Research Article
Information
Journal of Hygiene , Volume 83 , Issue 3 , December 1979 , pp. 507 - 512
Copyright
Copyright © Cambridge University Press 1979

References

Allison, A. C. (1972). Immunity against viruses. In The Scientific Basis of Medicine Annual Reviews (ed. Gilliland, I. and Francis, J.), pp. 49–73. Athlone Press, University of London.Google Scholar
Allison, A. C. (1974). Antibodies and cells in immunity. Transplantation Reviews 19, 3–55.Google Scholar
Babiuk, L. A., Wardley, R. C. & Rouse, B. T. (1975). Defense mechanisms against bovine herpesvirus: relationship of virus-host cell events to susceptibility to antibody-complement cell lysis. Infection and Immunity 12, 958–63.Google Scholar
Black, L. (1977). Allergy in cattle after foot and mouth disease vaccination. Veterinary Record 100, 195–8.Google Scholar
Brown, F. & Cartwright, B. (1963). Purification of radioactive foot-and-mouth disease virus. Nature, London 199, 1168.Google Scholar
Brown, F. & Crick, J. (1959). Application of agar gel diffusion analysis to a study of the antigenic structure of inactivated vaccines prepared from the virus of foot-and-mouth disease. Journal of Immunology 82, 444–7.Google Scholar
Martin, W. B. & Chapman, W. G. (1961). The tissue culture colour test for assaying the virus and neutralising antibody of foot-and-mouth disease and its application to the measurement of immunity in cattle. Research in Veterinary Science 2, 53–61.Google Scholar
Rouse, B. T. & Babiuk, L. A. (1975). Host defense mechanisms against infectious bovine rhinotracheitis virus. II. Inhibition of plaque formation by immune peripheral blood lymphocytes. Cellular Immunology 17, 43–56.Google Scholar
Van Bekkum, J. G. (1969). European Commission for the Control of Foot-and-Mouth Disease. Meeting of the Research Group of the Standing Technical Committee, Brescia, Italy. FAO, Rome.Google Scholar
Wardley, R. C. (1977). An improved E rosetting technique for cattle. British Veterinary Journal 133, 432–4.Google Scholar