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Apramycin-resistant Escherichia coli isolated from pigs and a stockman

Published online by Cambridge University Press:  15 May 2009

J. E. B. Hunter ,
M. Bennett ,
C. A. Hart ,
J. C. Shelley  and
J. R. Walton
J. E. B. Hunter
Affiliation:
Departments of Veterinary Clinical Science, University of Liverpool, PO Box 147, Liverpool L69 3BX Medical Microbiology2, University of Liverpool, PO Box 147, Liverpool L69 3BX
M. Bennett
Affiliation:
Departments of Veterinary Clinical Science, University of Liverpool, PO Box 147, Liverpool L69 3BX
C. A. Hart
Affiliation:
Medical Microbiology2, University of Liverpool, PO Box 147, Liverpool L69 3BX
J. C. Shelley
Affiliation:
Departments of Veterinary Clinical Science, University of Liverpool, PO Box 147, Liverpool L69 3BX
J. R. Walton
Affiliation:
Departments of Veterinary Clinical Science, University of Liverpool, PO Box 147, Liverpool L69 3BX
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Escherichia coli serotype O147:K89:K88a,c was found to be associated with outbreaks of diarrhoea in preweaner pigs of up to 4 weeks of age on a pig unit. Resistance to apramycin, gentamicin, netilmicin, tobramycin and other antibiotics was associated with conjugative plasmids of approximately 62 kb. The presence of a gene which encoded for the aminoglycoside acetyltransferase enzyme AAC(3)IV was confirmed by DNA hybridization.

Samples collected during the following 12 months revealed widespread dissemination of these resistance plasmids in non-serotypable, non-haemolytic E. coli throughout the farm. Apramycin-resistant E. coli were also isolated from a stockman and it appeared from plasmid profile analysis and antibiotic sensitivity testing that the human isolates carried the same plasmid as that carried by the porcine E. coli. Klebsiella pneumoniae, with a slightly smaller conjugative plasmid and similar resistance pattern, was isolated from the stockman's wife.

Type
Research Article
Information
Epidemiology & Infection , Volume 112 , Issue 3 , June 1994 , pp. 473 - 480
Copyright
Copyright © Cambridge University Press 1994

References

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