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Antigens recognized by the human immune response to severe leptospirosis in Barbados

Published online by Cambridge University Press:  15 May 2009

A. J. Chapman ,
C. O. R. Everard ,
S. Faine  and
B. Adler
A. J. Chapman
Affiliation:
Department of Microbiology, Monash University, Clayton, 3168, Victoria, Australia
C. O. R. Everard
Affiliation:
MRC/Barbados Govt, Leptospira Laboratory, Enmore 2, Lower Collymore Rock, St Michael, Barbados
S. Faine
Affiliation:
Department of Microbiology, Monash University, Clayton, 3168, Victoria, Australia
B. Adler*
Affiliation:
Department of Microbiology, Monash University, Clayton, 3168, Victoria, Australia
*
*Dr B. Adler, Department of Microbiology, Monash University, Clayton, 3168, Victoria, Australia
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Serum samples obtained from patients hospitalized in Barbados with severe leptospirosis were tested by the microscopic agglutination test (MAT), enzyme immunoassay (EIA) and immunoblotting with leptospires that had been isolated from these patients. While serum samples taken a few days after onset of symptoms often showed no apparent correlation between MAT and EIA, later sequential serum samples produced similar profiles in both tests during the course of infection. Immunoblotting sonicate from Leptospira interrogans serovars arborea, copenhageni and bim with patients' sera, revealed reactions with a number of bands that corresponded with outer envelope components. These components included lipopolysaccharide (LPS), flagella and other outer membrane proteins, in addition to a low-molecular-weight (MW) carbohydrate cross-reactive with members of the Leptospiraceae. IgM antibodies elicited in the first to second week after infection reacted mainly with LPS and the low-MW cross-reactive carbohydrate. Comparative analysis of isolates of the same serovar by sodium dodecyl sulphate polyacrylamide gel electrophoresis and immunoblotting showed that while two serovar arborea isolates were identical, serovar bim isolates differed significantly from each other. This difference was also observed in comparative MAT testing.

Type
Research Article
Information
Epidemiology & Infection , Volume 107 , Issue 1 , August 1991 , pp. 143 - 155
Copyright
Copyright © Cambridge University Press 1991

References

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