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An evaluation of strontium chloride, Rappaport and strontium selenite enrichment for the isolation of salmonellas from man, animals, meat products and abattoir effluents

Published online by Cambridge University Press:  15 May 2009

J. B. Iveson  and
E. M. Mackay-Scollay
J. B. Iveson
Affiliation:
Salmonella Diagnostic and Reference Laboratory, State Health Laboratory Service, Box F312, Perth, Western Australia
E. M. Mackay-Scollay
Affiliation:
Salmonella Diagnostic and Reference Laboratory, State Health Laboratory Service, Box F312, Perth, Western Australia
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Strontium chloride enrichment broth was found to be comparable to Rappaport broth for the recovery of a wide range of Salmonella serotypes from man, animals, meat products and effluents. With the exception of cloacal samples from reptiles, both procedures were superior to selenite F.

The performance of strontium chloride M and selenite F enrichment was improved when effluent samples were incubated at 43° C.

Strontium chloride M and Rappaport enrichment were superior to selenite F for the isolation of Arizona species from reptiles.

Strontium chloride B, strontium selenite and Rappaport broths were found suitable for the isolation of multiple Salmonella serotypes from sea water contaminated with abattoir effluents. The strontium chloride B and strontium selenite enrichment media were superior to Rappaport broth when samples were incubated at 43° C.

Modified bismuth sulphite agar was found superior to Salmonella-Shigella agar as a solid subculture medium.

The investigation of a food poisoning outbreak due to Salmonella typhimurium phage type 21 is reported.

The significance of the choice of sampling and isolation techniques in salmon-ellosis in man and animals is discussed.

Type
Research Article
Information
Journal of Hygiene , Volume 70 , Issue 2 , June 1972 , pp. 367 - 384
Copyright
Copyright © Cambridge University Press 1972

References

REFERENCES

Anderson, K. & Kennedy, H. (1965). Comparison of selective media for the isolation of salmonellae. Journal of Clinical Pathology 18, 747.Google Scholar
Atkinson, N. (1964). Salmonellosis in Australia. Reprinted from The World Problem of Salmonellosis. Ed. van Oye, E., Monographiae Biologica, Vol. xiii, p. 539. Dr W. Junk, The Hague.Google Scholar
Beh, K. J. H. (1971). The incidence of enteropathogenic Escherichia coli serotypes and Salmonella spp. in pigs in New South Wales. Australian Veterinary Journal 47, 379.Google Scholar
Chau, P. Y. & Huang, C. T. (1971). Carriage rate of Salmonella serotypes in hospital patients and comparison of enrichment media for their isolation. Tropical Medicine 13, 94.Google Scholar
Collard, P. & Unwin, M. (1958). A trial of Rappaport's medium. Journal of Clinical Pathology 11, 426.Google Scholar
Cruickshank, J. C. & Smith, H. Williams (1949). Isolation of Salmonellae from dogs, cats and pigeons. British Medical Journal ii, 1254.Google Scholar
Edel, W. & Kampelmacher, E. H. (1968). Comparative studies on Salmonella isolation in eight European laboratories. Bulletin of the World Health Organization 39, 487.Google Scholar
Edel, W. & Kampelmacher, E. H. (1969). Salmonella isolation in nine European laboratories using a standardised technique. Bulletin of the World Health Organization 41, 297.Google Scholar
Eisenburg, P. (1918). Untersuchungen über spezifische Desinfektionsvorgange. II. Ueber die Wirkung von Salzen und lonen auf Bakterien. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene (Abt. I, Orig.) 82, 69.Google Scholar
Galton, M. M., Scatterday, J. E. & Hardy, A. V. (1952). Salmonellosis in dogs: I. Bacteriological, epidemiological and clinical considerations. Journal of Infectious Diseases 91, 1.Google Scholar
Galton, M. M., Smith, W. V., McElrath, H. B. & Hardy, A. V. (1954). Salmonella in swine, cattle and the environment of abattoirs. Journal of Infectious Diseases 95, 236.Google Scholar
Gray, J. D. A. (1931). New lithium selective and enrichment methods for the isolation of Salmonella organisms. Journal of Pathology and Bacteriology 34, 335.Google Scholar
Harvey, R. W. S. & Phillips, W. P. (1961). An environmental survey of bakehouses and abattoirs, for salmonellae. Journal of Hygiene 59, 93.Google Scholar
Harvey, R. W. S. & Price, T. H. (1967). The examination of samples infected with multiple salmonella serotypes. Journal of Hygiene 65, 423.Google Scholar
Harvey, R. W. S. & Price, T. H. (1968). Elevated temperature incubation of enrichment media for the isolation of salmonellas from heavily contaminated materials. Journal of Hygiene 66, 377.Google Scholar
Harvey, R. W. S. & Price, T. H. (1970). Sewer and drain swabbing as a means of investigating Salmonellosis. Journal of Hygiene 68, 611.Google Scholar
Harvey, R. W. S. & Thomson, S. (1953). Optimum temperature of incubation for isolation of salmonellae. Monthly Bulletin of the Ministry of Health and the Public Health Laboratory Service 12, 149.Google Scholar
Hobbs, B. C. (1961). Public health significance of Salmonella carriers in livestock and birds. Journal of Applied Bacteriology 24, 340.Google Scholar
Hobbs, B. C. & Allison, V. D. (1945). Studies on the isolation of Bact. typhosum and Bact. paratyphosum B. Monthly Bulletin of the Ministry of Health and the Emergency Public Health Laboratory Service 4, 63.Google Scholar
Hooper, W. L. & Jenkins, H. R. (1965). An evaluation of Rappaport's magnesium chloride/malachite green medium in the routine examination of faeces. Journal of Hygiene 63, 491.Google Scholar
Hotchkiss, M. (1923). Studies on salt action. VI. The stimulatory and inhibitory effect of certain cations upon bacterial growth. Journal of Bacteriology 81, 141.Google Scholar
Iveson, J. B. (1971). Strontium chloride B and E.E. enrichment broth media for the isolation of Edwardsiella, Salmonella and Arizona species from tiger snakes. Journal of Hygiene 69, 323.Google Scholar
Iveson, J. B., Kovacs, N. & Laurie, W. (1964). An improved method for the isolation of salmonellae from dessicated coconut. Journal of Clinical Pathology 17, 75.Google Scholar
Iveson, J. B. & Kovacs, N. (1967). A comparative trial of Rappaport enrichment medium for the isolation of Salmonellae from faeces. Journal of Clinical Pathology 20, 290.Google Scholar
Iveson, J. B. & Mackay-Scollay, E. M. (1969). Strontium chloride and strontium selenite enrichment broth media in the isolation of Salmonella. Journal of Hygiene 67, 457.Google Scholar
Iveson, J. B., Mackay-Scollay, E. M. & Bamford, V. W. (1969). Salmonella and Arizona in reptiles and man in Western Australia. Journal of Hygiene 67, 135.Google Scholar
Kauffmann, F. (1930). Ein kombiniertes Anreicherungsverfahren für Typhus-und Para-typhusbazillen. Zentralblatt für Bakteriologie, Parisitenkunde und Infektionslcrankheiten (Abt. I, Orig.) 119, 148.Google Scholar
Kauffmann, F. (1935). Weitere Erfahrungen mit dem kombinierten Anreicherungsverfahren für Salmonellabacillen. Zeitschrifl für Hygiene und Infektionskrankheiten 117, 26.Google Scholar
Lee, P. E. & Mackerras, I. M. (1955). Salmonella infections of Australian native animals. Australian Journal of Experimental Biology and Medical Science 33, 117.Google Scholar
Leifson, E. (1936). New selenite enrichment media for the isolation of typhoid and paratyphoid (Salmonella) bacilli. American Journal of Hygiene 24, 423.Google Scholar
McDonagh, V. P. & Smith, H. G. (1958). The significance of the abattoir in Salmonella infection in Bradford. Journal of Hygiene 56, 271.Google Scholar
Muller, L. (1923). Un nouveau mileu d'enrichissement pour la recherce du bacilli typhique et des paratyphiques. Comptes rendus des séances de la Société de biologic et de ses filiales (Paris) 89, 434.Google Scholar
Rappaport, F., Konforti, N. & Navon, B. (1956). A new enrichment medium for certain salmonellae. Journal of Clinical Pathology 9, 261.Google Scholar
Riley, M. G. I. (1970). The incidence of Salmonella in normal slaughtered pigs. Australian Veterinary Journal 46, 40.Google Scholar
Rubbo, S. D. (1948). Cross-infection in hospital due to Salmonella derby. Journal of Hygiene 46, 158.Google Scholar
Sachs, A. (1939). Difficulties associated with the bacteriological diagnosis of bacillary dysentery. Journal of the Royal Army Medical Corps 73, 235.Google Scholar
Schothorst, Van M. & Kampelmacher, E. H. (1967). Salmonella in meat imported from South Amercian countries. Journal of Hygiene 65, 321.Google Scholar
Smith, H. Williams & Buxton, A. (1951). Isolation of salmonellae from faeces of domestic animals. British Medical Journal i, 1478.Google Scholar
Vassiliadis, P. (1968). Shigella spp., Salmonella cholerae-suis and Arizona in Rappaport's medium. Journal of Applied Bacteriology 31, 367.Google Scholar
Vassiliadis, P., Trichopoulos, D., Papadakis, J. & Politi, G. (1970). Salmonella isolations in abattoirs in Greece. Journal of Hygiene 68, 601.Google Scholar
Winkle, S. & Rohde, R. (1958). The diagnostic and epidemiological significance of mixed infections due to several salmonella serotypes. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene (Abt. I, Orig.) 173, 153.Google Scholar