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Preparation of Carboxymethyl Chitosan Microspheres and Their Application in Hemostasis

Published online by Cambridge University Press:  04 December 2015

Lu Liu ,
Qi Lv ,
Qingyun Zhang ,
Hui Zhu ,
Wei Liu ,
Guiru Deng ,
Yuqiang Wu ,
Chaofeng Shi ,
Hui Li  and
Lingzhi Li
Lu Liu*
Affiliation:
Logistical College of Chinese People’s Armed Police Forces, Tianjin, China
Qi Lv
Affiliation:
Institute of Disaster Medicine and Public Health, Affiliated Hospital of Chinese People’s Armed Police Force, Tianjin, China.
Qingyun Zhang
Affiliation:
Logistical College of Chinese People’s Armed Police Forces, Tianjin, China
Hui Zhu
Affiliation:
Logistical College of Chinese People’s Armed Police Forces, Tianjin, China
Wei Liu
Affiliation:
Logistical College of Chinese People’s Armed Police Forces, Tianjin, China
Guiru Deng
Affiliation:
Logistical College of Chinese People’s Armed Police Forces, Tianjin, China
Yuqiang Wu
Affiliation:
Logistical College of Chinese People’s Armed Police Forces, Tianjin, China
Chaofeng Shi
Affiliation:
Logistical College of Chinese People’s Armed Police Forces, Tianjin, China
Hui Li
Affiliation:
Logistical College of Chinese People’s Armed Police Forces, Tianjin, China
Lingzhi Li
Affiliation:
Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazard, Tianjin, China Institute of Disaster Medicine and Public Health, Affiliated Hospital of Chinese People’s Armed Police Force, Tianjin, China.
*
Correspondence and reprint requests to Dr Li Lingzhi, HuiZhiHuan Road 1, DongLi District, Tianjin, 300309, China (e-mail: 13682196000@163.com).
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Abstract

Objective

Chitosan (CS) is currently used as a hemostatic agent in emergencies and in military settings. However, its application is limited owing to its poor hydrophilia at neutral pH. Carboxymethyl chitosan (CMCS) is an important, water-soluble derivative of CS. In this study, we prepared CS and CMCS microspheres (CSMs and CMCSMs, respectively) and evaluated their hemostatic effect.

Methods

To prepare the microspheres of various sizes, we used the emulsion cross-linking technique. CMCSMs were also loaded with etamsylate (DIC). Clotting time in vitro and in a hepatic injury model was examined to evaluate the hemostatic effect.

Results

CMCSMs swelled more and clotted faster than did CSMs. CMCSMs loaded with DIC had no effect on hemostasis.

Conclusions

Both increasing material hydrophilicity and expanding the contact area promoted clotting, whereas chemical cross-linking hampered it because of decreased swelling. CMCSMs are promising candidates for the production of effective hemostatic agents. (Disaster Med Public Health Preparedness. 2017;11:660–667)

Keywords

emergency medicinemilitary medicineprotective deviceshemostatic agent in vitromicrosphere
Type
Original Research
Information
Disaster Medicine and Public Health Preparedness , Volume 11 , Issue 6 , December 2017 , pp. 660 - 667
Copyright
Copyright © Society for Disaster Medicine and Public Health, Inc. 2015 

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References

1. Khor, E, Lim, LY. Implantable applications of chitin and chitosan. Biomaterials 2003;24(13):2339-2349.Google ScholarPubMed
2. Millner, RW, Lockhart, AS, Bird, H, et al. A new hemostatic agent: initial life-saving experience with Celox (chitosan) in cardiothoracic surgery. Ann Thorac Surg. 2009;87(2):13-14.Google ScholarPubMed
3. Martin, L, Wilson, CG, Koosha, F, et al. The release of model macromolecules may be controlled by the hydrophobicity of palmitoyl glycol chitosan hydrogels. J Control Release. 2002;80(1):87-100.CrossRefGoogle ScholarPubMed
4. Chen, XG, Park, HJ. Chemical characteristics of O-carboxymethyl chitosans related to the preparation conditions. Carbohydr Polym. 2003;53(4):355-359.CrossRefGoogle Scholar
5. Chen, R-N, Wang, GM, Chen, CH, et al. Development of N, O-(carboxymethyl) chitosan/collagen matrixes as a wound dressing. Biomacromolecules. 2006;7(4):1058-1064.CrossRefGoogle Scholar
6. Liu, C, Fan, W, Chen, X, et al. Self-assembled nanoparticles based on linoleic-acid modified carboxymethyl-chitosan as carrier of adriamycin (ADR). Curr Appl Phys. 2007;7:125-129.CrossRefGoogle Scholar
7. Li, Y, Zhang, S, Meng, X, et al. The preparation and characterization of a novel amphiphilic oleoyl-carboxymethyl chitosan self-assembled nanoparticles. Carbohydr Polym. 2011;83(1):130-136.CrossRefGoogle Scholar
8. Wu, W, Peng, C, Han, B. Hemostatic effect of o-carboxymethyl chitosan powder on the trauma surface in rats. Journal of Surgery Concepts and Practice. 2010;15(3):240-244.Google Scholar
9. Zhao, YW, Liu, L, Han, X, et al. Preparation of N, O-carboxymethyl chitosan with different substitutional degree and its application for hemostasis. Adv Mat Res. 2013;798–799:1061-1066.Google Scholar
10. Humphreys, MR, Castle, EP, Andrews, PE, et al. Microporous polysaccharide hemospheres for management of laparoscopic trocar injury to the spleen. Am J Surg. 2008;195(1):99-103.CrossRefGoogle ScholarPubMed
11. Li, X, Chen, S, Li, X, et al. An experimental study on screening metal ion of zeolite hemostat. Science Technology and Engineering. 2010;18(2):048.Google Scholar
12. Liu, C, Bian, L, Chen, C. Study on preparation of high water-absorbing porous corn starch through sequential hydrolysis method of hydrochloric acid and composite enzyme. Chemistry & Bioengineering. 2010;27(2):023.Google Scholar
13. Motamedi, M, Sagafinia, M. QuickClot combat gauze use for hemorrhage control in military trauma. Prehosp Disaster Med. 2011;26(3):237.CrossRefGoogle ScholarPubMed
14. Liqun, W, Lei, X, Shuwei, W, Jiacan, S. Mesoporous silicon dioxide microsphere/starch composite hemostatic dressing. Journal of Clinical Rehabilitative Tissue Engineering Research. 2000;15(29):5379-5383.Google Scholar
15. Yushan, S, Xin, L, Yue, W, et al. Research on haemostatic function of biological medical textiles. Technical Textiles 2000;18(123):008.Google Scholar
16. Matsuoka, T, Hildreth, J, Wisner, DH. Liver injury as a model of uncontrolled hemorrhagic shock: resuscitation with different hypertonic regimens. J Trauma Acute Care Surg. 1995;39(4):674-680.CrossRefGoogle Scholar
17. Ong, SY, Wu, J, Moochhala, SM, et al. Development of a chitosan-based wound dressing with improved hemostatic and antimicrobial properties. Biomaterials. 2008;29(32):4323-4332.CrossRefGoogle ScholarPubMed
18. Luo, Y, Teng, Z, Wang, Q. Development of zein nanoparticles coated with carboxymethyl chitosan for encapsulation and controlled release of vitamin D3. J Agric Food Chem. 2002;60(3):836-843.CrossRefGoogle Scholar
19. Kheirabadi, BS, FieldRidley, A, Pearson, R, et al. Comparative study of the efficacy of the common topical hemostatic agents with fibrin sealant in a rabbit aortic anastomosis model. J Surg Res. 2002;106(1):99-107.CrossRefGoogle Scholar
20. Murat, FJL, Le, CQ, Ereth, MH, et al. Evaluation of microporous polysaccharide hemospheres for parenchymal hemostasis during laparoscopic partial nephrectomy in the porcine model. JSLS. 2006;10(3):302-306.Google ScholarPubMed
21. Arnaud, F, Tomori, T, Carr, W, et al. Exothermic reaction in zeolite hemostatic dressings: QuikClot ACS and ACS+. Ann Biomed Eng. 2008;36(10):1708-1713.Google ScholarPubMed
22. Hu, H, Ying, L, Depo, Y. Comparative study of effects of xuejie superfine powders with different diameters and its drop on stopping bleeding. Zhongguo Zhong Yao Za Zhi. 2004;14(6):3.Google Scholar
23. Pusateri, AE, McCarthy, SJ, Gregory, KW, et al. Effect of a chitosan-based hemostatic dressing on blood loss and survival in a model of severe venous hemorrhage and hepatic injury in swine. J Trauma Acute Care Surg. 2003;54(1):177-182.CrossRefGoogle Scholar
24. Fu, D, Han, B, Dong, W, et al. Effects of carboxymethyl chitosan on the blood system of rats. Biochem Biophys Res Commun. 2011;408(1):110-114.CrossRefGoogle ScholarPubMed
25. Mackman, N, Taubman, M. Tissue factor past, present, and future. Arterioscler Thromb Vasc Biol. 2009;29(12):1986-1988.CrossRefGoogle ScholarPubMed
26. Busani, S, Cavazzuti, I, Marietta, M, et al. Strategies to control massive abdominal bleeding. Transplant Proc. 2008;40(4):1212-1215.CrossRefGoogle ScholarPubMed
27. Liu, B, Yan, E, Zhang, X, et al. A general method for the synthesis of monodisperse hollow inorganic organic hybrid microspheres with interior functionalized poly (methacrylic acid) shells. J Colloid Interface Sci. 2012;369(1):144-153.Google ScholarPubMed