Psychological construction has always been a key challenge in guiding innovation and entrepreneurship among college students. The reform of practical courses for innovation and entrepreneurship education for college students aims to improve their recognition and satisfaction with innovation and entrepreneurship courses, thereby alleviating the impact of anxiety.

Taking senior college students as the research object, this study aims to involve them in the reform of practical courses for innovation and entrepreneurship education for college students. A symptom self-evaluation scale was used to conduct a follow-up survey to explore the impact of the reform of practical courses for innovation and entrepreneurship education for college students on their anxiety psychology.

The reform of the innovation and entrepreneurship education practice course for college students has a positive impact on their anxiety psychology in all aspects, and there is a positive correlation between this course reform and alleviating their mental health. The anxiety rate of students participating in the reform of innovation and entrepreneurship education practical courses for college students is only 6.3%, and the distribution trend of these students’ innovation ability scores is 5.79 ± 1.78. The anxiety level of students who did not participate was 55.4%.

Through the reform of practical courses for innovation and entrepreneurship education for college students, we guide them to actively start businesses, regulate tense emotions, alleviate psychological pressure, reduce anxiety factors, and alleviate anxiety symptoms, thereby achieving psychological balance and forming a stable psychological state.