Hostname: page-component-848d4c4894-4hhp2 Total loading time: 0 Render date: 2024-04-30T18:39:13.146Z Has data issue: false hasContentIssue false
  • English
  • Français

Fighting Against Plant Saline Stress: Development of a Novel Bioactive Composite Based on Bentonite and L-Proline

Published online by Cambridge University Press:  01 January 2024

Danila Merino Open the ORCID record for Danila Merino [Opens in a new window] ,
María J. Iglesias ,
Andrea Y Mansilla ,
Claudia A. Casalongué  and
Vera A. Alvarez
Danila Merino*
Affiliation:
Facultad de Ingeniería, Grupo de Materiales Compuestos Termoplásticos (CoMP), Instituto de Investigaciones en Ciencia y Tecnología de Materiales (INTEMA), Universidad Nacional de Mar del Plata (UNMdP) y Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Colón 10850, (7600) Mar del Plata, Mar del Plata, Argentina Present Address: Present Address: Smart Materials Group, Itailian Institute of Technology (HT), Vai Morego 30, 16163 Genoa, Italy
María J. Iglesias
Affiliation:
Facultad de Ciencias Exactas y Naturales, Instituto de Investigaciones Biológicas, UE CONICET-UNMDP, Universidad Nacional de Mar del Plata, (7600) Mar del Plata, 3250, Deán Funes, Argentina
Andrea Y Mansilla
Affiliation:
Facultad de Ciencias Exactas y Naturales, Instituto de Investigaciones Biológicas, UE CONICET-UNMDP, Universidad Nacional de Mar del Plata, (7600) Mar del Plata, 3250, Deán Funes, Argentina
Claudia A. Casalongué
Affiliation:
Facultad de Ciencias Exactas y Naturales, Instituto de Investigaciones Biológicas, UE CONICET-UNMDP, Universidad Nacional de Mar del Plata, (7600) Mar del Plata, 3250, Deán Funes, Argentina
Vera A. Alvarez
Affiliation:
Facultad de Ingeniería, Grupo de Materiales Compuestos Termoplásticos (CoMP), Instituto de Investigaciones en Ciencia y Tecnología de Materiales (INTEMA), Universidad Nacional de Mar del Plata (UNMdP) y Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Colón 10850, (7600) Mar del Plata, Mar del Plata, Argentina
*
*E-mail address of corresponding author: danila.merino@fi.mdp.edu.ar danila.merino@iit.it
Get access Rights & Permissions [Opens in a new window]

Abstract

Soil salinity is one of the most critical environmental stresses that affects crop productivity. In a context in which world demand for food is growing continuously, this problem requires urgent attention. Actions that go beyond traditional agricultural practices are needed. The objective of the current study was to develop a bioactive, economic, and sustainable compound that can increase the tolerance of cultivated plants in saline-stress situations by combining the hosting capacity of natural bentonite nanoclay (Bent) with a phytoactive osmoprotective compound, L-Proline (Pro). The Bent-Pro nanocomposite synthesis method, its final chemical structure, and in vitro bioactivity were addressed here. The results indicated that Bent can retain a maximum of 14.4% (w/w) of Pro. The (001) X-ray diffraction (XRD) peak of Bent shifted to smaller angles in the pattern of Bent-Pro, indicating that Pro has a monolayer arrangement between the Bent layers. The results of transmission electron microscopy (TEM) also supported this result. Pro was also retained on the edges or external surfaces of Bent, as indicated by thermogravimetric analysis (TGA) and scanning electron microscopy (SEM). In addition, Pro functional groups identified by Fourier-transform infrared (FTIR) spectroscopy indicated that it was present in its zwitterionic form. The role of Bent-Pro as a protector against plant saline stress was assayed using Arabidopsis thaliana (A. thaliana) as a model, demonstrating that it mitigates the detrimental effects of NaCl-mediated salt stress on seed germination and the leaf chlorophyll level, thus highlighting the relevance of this contribution and the versatility and broad applicability of clays.

Keywords

BentoniteBiomaterialL-prolinePlant acclimationSalt stressZwitterion
Type
Article
Information
Clays and Clay Minerals , Volume 69 , Issue 2 , April 2021 , pp. 232 - 242
Copyright
Copyright © Clay Minerals Society 2021

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Alexandre, M., & Dubois, P. (2000). Polymer-layered silicate nanocomposites: preparation, properties and uses of a new class of materials. Materials Science and Engineering: R: Reports, 28, 1–63.CrossRefGoogle Scholar
Arnon, D. I. (1949). Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiology, 24, 1–15.CrossRefGoogle ScholarPubMed
Ashraf, M., & Foolad, M. R. (2007). Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environmental and Experimental Botany, 59, 206–216.CrossRefGoogle Scholar
Bates, L. S., Waldren, R. P., & Teare, I. D. (1973). Rapid determination of free proline for water-stress studies. Plant and Soil, 39, 205–207.CrossRefGoogle Scholar
Boyes, D. C., Zayed, A. M., Ascenzi, R., McCaskill, A. J., Hoffman, N. E., Davis, K. R., & Görlach, J. (2001). Growth stage–based phenotypic analysis of Arabidopsis. The Plant Cell, 13, 1499LP–1510.Google ScholarPubMed
Bragg, W. H., & Bragg, W. L. (1913). The reflection of X-rays by crystals. Proceedings of the Royal Society of London A: Mathematical, Physical and Engineering Sciences, 88, 428–438.Google Scholar
Celis, R., Hermosín, M. C., Carrizosa, M. J., & Cornejo, J. (2002). Inorganic and organic clays as carriers for controlled release of the herbicide hexazinone. Journal of Agricultural and Food Chemistry, 50, 2324–2330.CrossRefGoogle ScholarPubMed
Chen, H., Chen, Q. S., Huang, B., Wang, S. W., & Wang, L. Y. (2018). High-potential use of l-Cysh modified bentonite for efficient removal of U(VI). from aqueous solution. Journal of Radioanalytical and Nuclear Chemistry, 316, 71–80.CrossRefGoogle Scholar
de Campos, M. K. F., de Carvalho, K., de Souza, F. S., Marur, C. J., Pereira, L. F. P., Filho, J. C. B., & Vieira, L. G. E. (2011). Drought tolerance and antioxidant enzymatic activity in transgenic ‘Swingle’ citrumelo plants overaccumulating proline. Environmental and Experimental Botany, 72, 242–250.CrossRefGoogle Scholar
Deivanai, S., Xavier, R., Vinod, V., Timalata, K., & Lim, O. F. (2011). Role of exogenous proline in ameliorating salt stress at early stage in two rice cultivars. Journal of Stress Physiology & Biochemistry, 7, 157–174.Google Scholar
Devi, T. U., Lawrence, N., Ramesh Babu, R., Selvanayagam, S., Stoeckli-Evans, H., & Ramamurthi, K. (2009). Synthesis, crystal growth and characterization of l -Proline lithium chloride monohydrate: A new semiorganic nonlinear optical material. Crystal Growth & Design, 9, 1370–1374.CrossRefGoogle Scholar
El Moukhtari, A., Cabassa-Hourton, C., Farissi, M., & Savouré, A. (2020). How does proline treatment promote salt stress tolerance during crop plant development? Frontiers in Plant Science, 11, 1127.CrossRefGoogle ScholarPubMed
Food and Agriculture Organization of the United Nations. (2017). The future of food and agriculture: Trends and challenges. http://www.fao.org/3/I8429EN/i8429en.pdfGoogle Scholar
Gamba, M., Flores, F. M., Madejová, J., & Torres Sánchez, R. M. (2015). Comparison of imazalil removal onto montmorillonite and nanomontmorillonite and adsorption surface sites involved: an approach for agricultural wastewater treatment. Industrial & Engineering Chemistry Research, 54, 1529–1538.CrossRefGoogle Scholar
Golldack, D., Li, C., Mohan, H., & Probst, N. (2014). Tolerance to drought and salt stress in plants: unraveling the signaling networks. Frontiers in Plant Science, 5, 151.CrossRefGoogle ScholarPubMed
Grillo, R., Mattos, B. D., Antunes, D. R., Forini, M. M. L., Monikh, F. A., & Rojas, O. J. (2021). Foliage adhesion and interactions with particulate delivery systems for plant nanobionics and intelligent agriculture. Nano Today, 37, 101078.CrossRefGoogle Scholar
Hayat, S., Hayat, Q., Alyemeni, M. N., Wani, A. S., Pichtel, J., & Ahmad, A. (2012). Role of proline under changing environments: a review. Plant Signaling & Behavior, 7, 1456–1466.CrossRefGoogle ScholarPubMed
Iglesias, M. J., Terrile, M. C., Bartoli, C. G., D'Ippólito, S., & Casalongué, C. A. (2010). Auxin signaling participates in the adaptative response against oxidative stress and salinity by interacting with redox metabolism in Arabidopsis. Plant Molecular Biology, 74, 215–222.CrossRefGoogle ScholarPubMed
Iglesias, M. J., Terrile, M. C., Windels, D., Lombardo, M. C., Bartoli, C. G., Vazquez, F., Estelle, M., & Casalongué, C. A. (2014). MiR393 regulation of auxin signaling and redox-related components during acclimation to salinity in Arabidopsis. PLoS ONE, 9, e107678.CrossRefGoogle ScholarPubMed
Jafarbeglou, M., Abdouss, M., Shoushtari, A. M., & Jafarbeglou, M. (2016). Clay nanocomposites as engineered drug delivery systems. RSC Advances, 6, 50002–50016.CrossRefGoogle Scholar
Kaur, G., & Asthir, B. (2015). Proline: a key player in plant abiotic stress tolerance. Biologia Plantarum, 59, 609–619.CrossRefGoogle Scholar
Kishor, P. B. K., Hong, Z., Miao, G. H., Hu, C. A. A., & Verma, D. P. S. (1995). Overexpression of δ-pyrroline-5-carboxylate synthetase increases proline production and confers osmotolerance in transgenic plants. Plant Physiology, 108, 1387LP–1394.CrossRefGoogle ScholarPubMed
Komadel, P., & Madejová, J. (2006). Acid activation of clay minerals. In Handbook of Clay Science (Bergaya, F., Theng, B.K.G., and Lagaly, G., (Ed.), Developments in Clay Science. (Vol. 1, pp. 263–287). Elsevier.CrossRefGoogle Scholar
Lagaly, G. (2001). Pesticide–clay interactions and formulations. Applied Clay Science, 18, 205–209.CrossRefGoogle Scholar
Li, J., Li, Y., & Dong, H. (2008). Controlled Release of Herbicide Acetochlor from Clay/Carboxylmethylcellulose Gel Formulations. Journal of Agricultural and Food Chemistry, 56, 1336–1342.CrossRefGoogle ScholarPubMed
Lu, B.-R. (2016). Challenges of transgenic crop commercialization in China. Nature Plants, 2, 16077.CrossRefGoogle ScholarPubMed
Mallakpour, S., & Dinari, M. (2011). Preparation and characterization of new organoclays using natural amino acids and cloisite Na+. Applied Clay Science, 51, 353–359.CrossRefGoogle Scholar
Mansilla, A. Y., Salcedo, M. F., Colman, S. L., Chevalier, M. T., Lanfranconi, M. R., Alvarez, V. A., & Casalongué, C. A. (2020). Characterization of functionalized bentonite as nanocarrier of salicylic acid with protective action against Pseudomonas syringae in tomato plants. European Journal of Plant Pathology, 158, 211–222.CrossRefGoogle Scholar
McConnell, D. (1950). The crystal chemistry of montmorillonite. American Mineralogist, 35, 166–172.Google Scholar
McKee, T., & McKee, J. (2003). Biochemistry: The Molecular Basis of Life. McGraw-Hill.Google Scholar
Merino, D., & Alvarez, V. A. (2020). Thermal degradation of poly (ε-caprolactone). nanocomposites with soy lecithinmodified bentonite fillers. Thermochimica Acta, 689, 178638.CrossRefGoogle Scholar
Merino, D., Ollier, R., Lanfranconi, M., & Alvarez, V. (2016). Preparation and characterization of soy lecithin-modified bentonites. Applied Clay Science, 127, 17–22.CrossRefGoogle Scholar
Merino, D., Mansilla, A. Y., Casalongué, C. A., & Alvarez, V. A. (2018). Preparation, characterization, and in vitro testing of nanoclay antimicrobial activities and elicitor capacity. Journal of Agricultural and Food Chemistry, 66(12), 3101–3109.CrossRefGoogle ScholarPubMed
Merino, D., Tomadoni, B., Salcedo, M. F., Mansilla, A. Y., Casalongué, C. A., & Alvarez, V. A. (2020). Nanoclay as carriers of bioactive molecules applied to agriculture. Pp. 1–22 in: Handbook of Nanomaterials and Nanocomposites for Energy and Environmental Applications (O.V. Kharissova, L.M.T. Martínez, and B.I. Kharisov). Springer, Berlin.Google Scholar
Nanjo, T., Fujita, M., Seki, M., Kato, T., Tabata, S., & Shinozaki, K. (2003). Toxicity of free proline revealed in an arabidopsis T-DNA-tagged mutant deficient in proline dehydrogenase. Plant & Cell Physiology, 44, 541–548.CrossRefGoogle Scholar
Negrão, S., Schmöckel, S. M., & Tester, M. (2016). Evaluating physiological responses of plants to salinity stress. Annals of Botany, 119, 1–11.CrossRefGoogle ScholarPubMed
Ni, B., Liu, M., Lü, S., Xie, L., & Wang, Y. (2011). Environmentally Friendly Slow-Release Nitrogen Fertilizer. Journal of Agricultural and Food Chemistry, 59, 10169–10175.CrossRefGoogle ScholarPubMed
Parbhakar, A., Cuadros, J., Sephton, M. A., Dubbin, W., Coles, B. J., & Weiss, D. (2007). Adsorption of l-lysine on montmorillonite. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 307, 142–149.CrossRefGoogle Scholar
Patel, H. A., Somani, R. S., Bajaj, H. C., & Jasra, R. V. (2006). Nanoclays for polymer nanocomposites, paints, inks, greases and cosmetics formulations, drug delivery vehicle and waste water treatment. Bulletin of Materials Science, 29, 133–145.CrossRefGoogle Scholar
Pédro, G. (1965). La constitution cristallochimique des phyllosilicates. Sa signification et ses conséquences. Bulletin du Groupe français des argiles, 16, 37–47.CrossRefGoogle Scholar
Per, T. S., Khan, N. A., Reddy, P. S., Masood, A., Hasanuzzaman, M., Khan, M. I. R., & Anjum, N. A. (2017). Approaches in modulating proline metabolism in plants for salt and drought stress tolerance: Phytohormones, mineral nutrients and transgenics. Plant Physiology and Biochemistry, 115, 126–140.CrossRefGoogle ScholarPubMed
Petra, L., Billik, P., & Komadel, P. (2015). Preparation and characterization of hybrid materials consisting of highenergy ground montmorillonite and α-amino acids. Applied Clay Science, 115, 174–178.CrossRefGoogle Scholar
Ruiz-Hitzky, E., Aranda, P., Darder, M., & Rytwo, G. (2010). Hybrid materials based on clays for environmental and biomedical applications. Journal of Materials Chemistry, 20, 9306–9321.CrossRefGoogle Scholar
Saha, J., Brauer, E. K., Sengupta, A., Popescu, S. C., Gupta, K., & Gupta, B. (2015). Polyamines as redox homeostasis regulators during salt stress in plants. Frontiers in Environmental Science, 3, 21.CrossRefGoogle Scholar
Sanchez-Martin, M. J., Rodriguez-Cruz, M. S., Andrades, M. S., & Sanchez-Camazano, M. (2006). Efficiency of different clay minerals modified with a cationic surfactant in the adsorption of pesticides: influence of clay type and pesticide hydrophobicity. Applied Clay Science, 31, 216–228.CrossRefGoogle Scholar
Shahid, S. A., Zaman, M., & Heng, L. (2018). Introduction to soil salinity, sodicity and diagnostics techniques. Pp. 1–42 in: Guideline for Salinity Assessment, Mitigation and Adaptation Using Nuclear and Related Techniques (Zaman, M., Shahid, S.A., and Heng, L., editors). Springer International Publishing, Cham.Google Scholar
Shokri, E., Yegani, R., & Akbarzadeh, A. (2017). Novel adsorptive mixed matrix membranes by embedding modified montmorillonite with arginine amino acid into polysulfones for As(V) removal. Applied Clay Science, 144, 141–149.CrossRefGoogle Scholar
Surekha, C., Kumari, K. N., Aruna, L. V., Suneetha, G., Arundhati, A., & Kavi Kishor, P. B. (2014). Expression of the Vigna aconitifolia P5CSF129A gene in transgenic pigeonpea enhances proline accumulation and salt tolerance. Plant Cell. Tissue and Organ Culture (PCTOC), 116, 27–36.CrossRefGoogle Scholar
Surender Reddy, P., Jogeswar, G., Rasineni, G. K., Maheswari, M., Reddy, A. R., Varshney, R. K., & Kavi Kishor, P. B. (2015). Proline over-accumulation alleviates salt stress and protects photosynthetic and antioxidant enzyme activities in transgenic sorghum [Sorghum bicolor (L.). Moench]. Plant Physiology and Biochemistry, 94, 104–113.CrossRefGoogle Scholar
Szabados, L., & Savouré, A. (2010). Proline: a multifunctional amino acid. Trends in Plant Science, 15, 89–97.CrossRefGoogle ScholarPubMed
Wu, C.-J., Gaharwar, A. K., Schexnailder, P. J., & Schmidt, G. (2010). Development of biomedical polymer-silicate nanocomposites: A materials science perspective. Materials, 3, 2986–3005.CrossRefGoogle Scholar
Zhang, J. Z., Creelman, R. A., & Zhu, J.-K. (2004). From laboratory to field. Using information from Arabidopsis to engineer salt, cold, and drought tolerance in crops. Plant Physiology, 135, 615LP–621.CrossRefGoogle ScholarPubMed
Zhu, J.-K. (2002). Salt and drought stress signal transduction in plants. Annual Review of Plant Biology, 53, 247–273.CrossRefGoogle ScholarPubMed
Zhu, J., Zhang, P., Qing, Y., Wen, K., Su, X., Ma, L., Wei, J., Liu, H., He, H., & Xi, Y. (2017). Novel intercalation mechanism of zwitterionic surfactant modified montmorillonites. Applied Clay Science, 141, 265–271.CrossRefGoogle Scholar
Supplementary material: File

Merino et al. supplementary material
Download undefined(File)
File 98.7 KB