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Cross-Linked Hydroxy-Al-Montmorillonite as a Stationary Phase in Liquid Chromatography

Published online by Cambridge University Press:  02 April 2024

Faina Tsvetkov ,
U. Mingelgrin  and
N. Lahav
Faina Tsvetkov
Affiliation:
Institute of Soils and Water, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan 50-250, Israel
U. Mingelgrin
Affiliation:
Institute of Soils and Water, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan 50-250, Israel
N. Lahav
Affiliation:
The Seagram Center for Soil and Water Sciences, The Faculty of Agriculture, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76-100, Israel
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Abstract

Quasi-spherical particles (7-µm mean diameter) were prepared from cross-linked hydroxy-Al-montmorillonite (basal spacing =15.3 and 18.6 Å) by spray-drying. These particles (SP-CLM) were used as a packing material for columns in high-pressure liquid chromatography (HPLC). Aromatic phosphate esters, chlorosubstituted phenyl-ureas, monosubstituted benzenes, and the o-, m-, and p-isomers of disubstituted benzenes were separated on the columns, using isopropanol and hexane. Alkyl and alkoxy groups in the solute molecules sterically hindered the interaction of these molecules with the solid phase. The retention of the isomers of disubstituted benzenes that possess one electronegative (or dipolar) and one nonpolar substituent increased in the order: o- < m- < p-. In contrast, for all investigated disubstituted benzenes containing two electronegative (or dipolar) substituents, the o-isomer was retained on the 18.6-Å SP-CLM more strongly than the m- and p-isomers. The chromatographic data suggest that the strength of the interaction of phenolic groups with the solid phase increased with the acidity of these groups. The o-isomers of phenols that contained an additional electronegative (or dipolar) substituent displayed an exceptionally strong adsorption. The capacity of the o-isomers of disubstituted benzenes containing two electronegative (or dipolar) substituents to form chelates with Al probably was responsible for the strong retention of such o-isomers compared with the retention of the corresponding m- and p-isomers.

Cross-linked hydroxy-Al-montmorillonite acted as a strong and selective adsorbent for many families of organic compounds. It was used successfully as the solid phase in HPLC separations with eluents ranging from nonpolar (e.g., hexane) to highly polar (e.g., water).

Keywords

AdsorptionChelationCross-linked smectiteDisubstituted benzeneHigh-pressure liquid chromatographyHydroxy-AlMonosubstituted benzenePhenyl-ureaPhosphate esters
Type
Research Article
Information
Clays and Clay Minerals , Volume 38 , Issue 4 , August 1990 , pp. 380 - 390
Copyright
Copyright © 1990, The Clay Minerals Society

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