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Binding of DNA to Natural Sepiolite: Applications in Biotechnology and Perspectives

Published online by Cambridge University Press:  01 January 2024

Sandrine Ragu ,
Olivier Piétrement  and
Bernard S. Lopez Open the ORCID record for Bernard S. Lopez [Opens in a new window]
Sandrine Ragu
Affiliation:
Université de Paris, INSERM U1016, UMR 8104 CNRS, Institut Cochin, Equipe Labellisée Ligue Contre le Cancer, Paris, France
Olivier Piétrement
Affiliation:
Laboratoire Interdisciplinaire Carnot de Bourgogne, CNRS UMR 6303, Université de Bourgogne, 9 Avenue Alain Savary, 21078 Dijon Cedex, France
Bernard S. Lopez*
Affiliation:
Université de Paris, INSERM U1016, UMR 8104 CNRS, Institut Cochin, Equipe Labellisée Ligue Contre le Cancer, Paris, France
*
*E-mail address of corresponding author: bernard.lopez@inserm.fr
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Abstract

DNA manipulation is crucial for many biotechnological prospects and for medical applications such as gene therapy. This requires the amplification and extraction of DNA from bacteria and the transfer of these DNA molecules into cells, including bacterial and mammalian cells. The capacity of the natural magnesium silicate clay mineral sepiolite to bind to DNA makes it a potentially useful tool for biotechnological/medical strategies. In addition, sepiolite is inexpensive and classified as non-toxic and non-carcinogenic. This review will first describe the physicochemical interactions between sepiolite and DNA. Then, the leverage of sepiolite/DNA interactions for DNA extraction from bacteria, to optimize DNA transfer into bacteria and DNA transfection into mammalian cells, are presented. Finally, the putative toxicity of sepiolite and its advantages and perspectives for future prospects, such as the improvement of immunotherapy, are also discussed.

Keywords

Bacterial transformationDNA interactionDNA plasmid extractionDNA transfection in mammalian cellsSepiolite
Type
Article
Information
Clays and Clay Minerals , Volume 69 , Issue 5: Clay Minerals in Health Applications , October 2021 , pp. 633 - 640
Copyright
Copyright © The Clay Minerals Society 2021

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Footnotes

Sandrine Ragu and Olivier Piétrement contributed equally to the writing of this paper

This paper belongs to a special issue on ‘Clay Minerals in Health Applications’

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