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PCR for the detection of the anti-herbicide genes in genetically modified organisms

Published online by Cambridge University Press:  12 February 2007

Xu Wen-Tao ,
Huang Kun-Lun ,
Deng Ai-Ke  and
Luo Yun-Bo
Xu Wen-Tao*
Affiliation:
College of Food Science and Nutrition Engineering, China Agricultural University, Beijing 100083, China
Huang Kun-Lun*
Affiliation:
College of Food Science and Nutrition Engineering, China Agricultural University, Beijing 100083, China
Deng Ai-Ke
Affiliation:
College of Food Science and Nutrition Engineering, China Agricultural University, Beijing 100083, China
Luo Yun-Bo*
Affiliation:
College of Food Science and Nutrition Engineering, China Agricultural University, Beijing 100083, China
*
**These authors contributed equally to this work.
**These authors contributed equally to this work.
*Corresponding author: E-mail: lyb@cau.edu.cn
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Abstract

Genetically modified organisms (GMOs) containing anti-herbicide genes account for more than 75% of all GMO and the proportion is increasing. The polymerase chain reaction (PCR) method has proved to be an invaluable tool for the specific and sensitive detection of genetically modified material in foodstuffs and PCR screening for the presence of transgenic components in food is becoming a routine method in modern food analysis. In this study, the five kinds of the anti-herbicide genes (bar, pat, cp4-epsps1, cp4epsps2 and gox) were examined by PCR in four kinds of genetically modified crops (soy, maize, cotton and rape). Results indicate that the method was sensitive, specific and credible, and that all genetically modified and approved crops containing the anti-herbicide genes can be examined by this method.

Keywords

anti-herbicide genegenetically modified organismpolymerase chain reaction
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 3 , Issue 1 , April 2006 , pp. 53 - 57
Copyright
Copyright © China Agricultural University and Cambridge University Press 2006

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