Hostname: page-component-7c8c6479df-8mjnm Total loading time: 0 Render date: 2024-03-29T23:00:08.604Z Has data issue: false hasContentIssue false

Karyotyping of Brassica oleracea C genome using Brassica A genomic DNA as blocking agent

Published online by Cambridge University Press:  29 January 2010

Chen Xiao-Dan
Affiliation:
College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China
Zhu Li-Quan*
Affiliation:
College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China
Wang Yong
Affiliation:
College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China
Rong Xiao-Ying
Affiliation:
College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China
Lu Jun
Affiliation:
College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China
Wang Xiao-Jia
Affiliation:
College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China
*
*Corresponding author. E-mail: zhuliquan@swu.edu.cn

Abstract

In exploring an effective and reliable karyotyping method in Brassica crop plants, Brassica oleracea was successfully karyotyped using genomic in situ hybridization (GISH). B. oleracea genomic DNA was labelled as probe using DIG-high prime mix kit, with B. rapa genomic DNA acting as blocking agent. Specific fluorescent signals were detected on each pair of homologous chromosomes, and nine pairs of chromosomes of B. oleracea were clearly identified according to the signal characteristics. A practical and accurate method for conducting karyotyping of small chromosomes has been demonstrated.

Type
Research Papers
Copyright
Copyright © China Agricultural University 2009

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Armstrong, SJ, Fransz, P, Marshall, DF and Jones, GH (1998) Physical mapping of DNA repetitive sequences to mitotic and meiotic chromosomes of Brassica oleracea var. Alboglabra by fluorescence in situ hybridization. Heredity 81: 666673.CrossRefGoogle Scholar
Axelsson, T, Bowman, CM, Sharpe, AG, Lydiate, DJ and Lagercrantz, U (2000) Amphidiploid Brassica juncea contains conserved progenitor genomes. Genome 43: 679688.CrossRefGoogle ScholarPubMed
Chen, LL, Peng, GZ, Zhang, WL and Tian, DC (2006a) The significance of mutations in genomic evolution. Hereditas (Beijing) 28: 631638.Google ScholarPubMed
Chen, HG, Cheng, YG, Liu, C and Wu, JS (2006b) Development and GISH analysis of amphidiploid Raphanobrassica between Raphanus sativus and Brassica alboglabra. Acta Agronomica Sinica 32: 11171120.Google Scholar
Cheng, YG, Hua, YW, Zhang, MH, Chen, HG and Wu, JS (2006) Genomic in situ hybridization in intergeneric hybrids between Raphanus sativus and Brassica oleracea. Hereditas (Beijing) 28: 858864.Google ScholarPubMed
Hasterok, R, Jenkins, G, Langdon, T, Jones, RN and Maluszynska, J (2001) Ribosomal DNA is an effective marker of Brassica chromosomes. Theoretical and Applied Genetics 103: 486490.CrossRefGoogle Scholar
Kamisugi, Y, Nakayama, S, O'Neil, CM, Mathias, RJ, Trick, M and Fukui, K (1998) Visualization of the Brassica self-incompatibility S-locus on identified oilseed rape chromosomes. Plant Molecular Biology 38: 10811087.CrossRefGoogle ScholarPubMed
Lagercrantz, U and Lydiate, DJ (1996) Comparative genome mapping in Brassica. Genetics 144: 19031910.CrossRefGoogle ScholarPubMed
Li, MX (1985) Investigative Technology of Plant Chromosomes. Haerbin: Northeast Forestry University Press.Google Scholar
Li, MX and Chen, RY (1985) A suggestion on the standardization of karyotype analysis in plants. Journal of Wuhan Botanical Research 3: 297302.Google Scholar
Li, ZY, Ceccarelli, M, Minelli, S, Contento, A, Liu, Y and Cionini, PG (2002a) High frequency to generate aneuploid and homozygous plants of Brassicaceae and genomic in situ hybridization (GISH) analysis. Science in China (Series C) 32: 218225.Google Scholar
Li, ZY, Li, MT, Huang, RG, Wu, XM and Song, YC (2002b) Genomic in situ hybridization (GISH) discriminates the A, B and C genomes in Brassica allotetraploid species. Chinese Journal of Oil Crop Sciences 24: 1014.Google Scholar
Li, ZY, Wu, XM, Wang, XQ and Song, YC (2003) Genomic in situ hybridization (GISH) analysis of B. oleracea and 5 related Brassica species. Chinese Journal of Oil Crop Sciences 25: 1620.Google Scholar
Long, M, Betran, E, Thornton, K and Wang, W (2003) The origin of new genes: glimpses from the young and old. Nature Review Genetics 4: 865875.CrossRefGoogle ScholarPubMed
Qi, HY (2006) Analysis of Chromosome and Localization of the S-receptor kinase (SRK) gene sequence in Brassica oleracea by FISH. Chongqing: Southwest University 3036.Google Scholar
Schwarzacher, T, Anamthawat-Jonsson, K, Harrison, GE, et al. (1992) Genomic in situ hybridization to identify alien chromosomes and chromosome segments in wheat. Theoretical and Applied Genetics 84: 778786.CrossRefGoogle ScholarPubMed
Snowdon, RJ, Kobler, W and Kohler, A (1997) Chromosomal localization and characterization of rDNA loci in the Brassica A and C genomes. Genome 40: 582587.CrossRefGoogle ScholarPubMed
Song, KM, Osborn, TC and Williams, PH (1988) Brassica taxonomy based on nuclear restriction fragment length polymorphisms (RFLPs) 1. Genome evolution of diploid and amphidiploid species. Theoretical and Applied Genetics 75: 784794.CrossRefGoogle Scholar
Warwick, SL and Black, LD (1991) Molecular systematics of Brassica and allied genera (subtribe Brassicinae, Brassiceae)-chloroplast genome and cytodeme dongruence. Theoretical and Applied Genetics 82: 8192.CrossRefGoogle Scholar
Wang, TX, Wu, CH, Huang, JY and Wei, WH (2006) Karyotyping of Brassica oleracea L. based on rDNA and Cot-1 DNA fluorescence in situ hybridization. Journal of Wuhan University (Natural Science Edition) 52: 230234.Google Scholar
Zhu, LQ and Wang, XJ (2000) The rapid analysis of self-incompatibility and its F1 lines of Brassica oleracea L. by using the polymorphism of S locus. Acta Botanica Sinica 42: 595599.Google Scholar
Zhu, WS, Xu, J, Hua, YW and Li, ZY (2005) Genomic analysis of intergeneric hybrids between Brassica carinata and Orychophragmus violaceus by using in situ hybridization. Acta Botanica Boreal-Occident Sinica 25: 662667.Google Scholar