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Construction and evaluation of near-isogenic lines for major QTLs of basal root thickness and 1000-grain-weight in lowland and upland rice

Published online by Cambridge University Press:  13 February 2008

Liu Li-Feng ,
Zhang Hong-Liang ,
Mu Ping ,
Qu Yan-Ying  and
Li Zi-Chao
Liu Li-Feng
Affiliation:
Key Laboratory of Crop Genomics and Genetic Improvement of Ministry of Agriculture, Key Laboratory of Crop Heterosis and Utilization of Ministry of Education and Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100094, China College of Agronomy, Agricultural University of Hebei, Baoding 071001, China
Zhang Hong-Liang
Affiliation:
Key Laboratory of Crop Genomics and Genetic Improvement of Ministry of Agriculture, Key Laboratory of Crop Heterosis and Utilization of Ministry of Education and Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100094, China
Mu Ping
Affiliation:
Key Laboratory of Crop Genomics and Genetic Improvement of Ministry of Agriculture, Key Laboratory of Crop Heterosis and Utilization of Ministry of Education and Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100094, China
Qu Yan-Ying
Affiliation:
Key Laboratory of Crop Genomics and Genetic Improvement of Ministry of Agriculture, Key Laboratory of Crop Heterosis and Utilization of Ministry of Education and Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100094, China
Li Zi-Chao*
Affiliation:
Key Laboratory of Crop Genomics and Genetic Improvement of Ministry of Agriculture, Key Laboratory of Crop Heterosis and Utilization of Ministry of Education and Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100094, China
*
*Corresponding author. E-mail: lizichao@cau.edu.cn
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Abstract

The development of near-isogenic lines (NILs) is an important fundamental step in the cloning of quantitative trait loci (QTL) and molecular marker assisted breeding. In the present study, NILs for two major QTLs of basal root thickness (BRT) and 1000-grain-weight (TGW) were obtained by molecular marker-assisted selection (MAS) through foreground selection for target QTL and background selection in three backcross generations (BC1F1, BC2F1 and BC3F1). After phenotypic evaluation in the BC3F2 generation, nine BRT QTL-NILs with BRT ranges of 1.07–1.16 mm (6.11–15.18% greater than the recurrent parent) and an average recovery ratio of genetic background (RRGB) of 97.22%, and 11 TGW QTL-NILs with ranges of 21.25–26.25 g (7.05–32.16% greater than the recurrent parent) and 95.97% of RRGB, were selected.

Keywords

lowland riceupland ricebasal root thickness1000-grain-weightmolecular marker-assisted selectionQTL-near isogenic lines
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 4 , Issue 3 , December 2007 , pp. 199 - 205
Copyright
Copyright © China Agricultural University and Cambridge University Press 2007

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Footnotes

First published in Journal of Agricultural Biotechnology 2007, 15(3): 469–476

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