Hostname: page-component-848d4c4894-jbqgn Total loading time: 0 Render date: 2024-06-30T18:48:32.540Z Has data issue: false hasContentIssue false
  • English
  • Français

Construction and evaluation of near-isogenic lines for major QTLs of basal root thickness and 1000-grain-weight in lowland and upland rice

Published online by Cambridge University Press:  13 February 2008

Liu Li-Feng ,
Zhang Hong-Liang ,
Mu Ping ,
Qu Yan-Ying  and
Li Zi-Chao
Liu Li-Feng
Affiliation:
Key Laboratory of Crop Genomics and Genetic Improvement of Ministry of Agriculture, Key Laboratory of Crop Heterosis and Utilization of Ministry of Education and Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100094, China College of Agronomy, Agricultural University of Hebei, Baoding 071001, China
Zhang Hong-Liang
Affiliation:
Key Laboratory of Crop Genomics and Genetic Improvement of Ministry of Agriculture, Key Laboratory of Crop Heterosis and Utilization of Ministry of Education and Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100094, China
Mu Ping
Affiliation:
Key Laboratory of Crop Genomics and Genetic Improvement of Ministry of Agriculture, Key Laboratory of Crop Heterosis and Utilization of Ministry of Education and Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100094, China
Qu Yan-Ying
Affiliation:
Key Laboratory of Crop Genomics and Genetic Improvement of Ministry of Agriculture, Key Laboratory of Crop Heterosis and Utilization of Ministry of Education and Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100094, China
Li Zi-Chao*
Affiliation:
Key Laboratory of Crop Genomics and Genetic Improvement of Ministry of Agriculture, Key Laboratory of Crop Heterosis and Utilization of Ministry of Education and Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100094, China
*
*Corresponding author. E-mail: lizichao@cau.edu.cn
Get access Rights & Permissions [Opens in a new window]

Abstract

The development of near-isogenic lines (NILs) is an important fundamental step in the cloning of quantitative trait loci (QTL) and molecular marker assisted breeding. In the present study, NILs for two major QTLs of basal root thickness (BRT) and 1000-grain-weight (TGW) were obtained by molecular marker-assisted selection (MAS) through foreground selection for target QTL and background selection in three backcross generations (BC1F1, BC2F1 and BC3F1). After phenotypic evaluation in the BC3F2 generation, nine BRT QTL-NILs with BRT ranges of 1.07–1.16 mm (6.11–15.18% greater than the recurrent parent) and an average recovery ratio of genetic background (RRGB) of 97.22%, and 11 TGW QTL-NILs with ranges of 21.25–26.25 g (7.05–32.16% greater than the recurrent parent) and 95.97% of RRGB, were selected.

Keywords

lowland riceupland ricebasal root thickness1000-grain-weightmolecular marker-assisted selectionQTL-near isogenic lines
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 4 , Issue 3 , December 2007 , pp. 199 - 205
Copyright
Copyright © China Agricultural University and Cambridge University Press 2007

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

First published in Journal of Agricultural Biotechnology 2007, 15(3): 469–476

References

Boerma, HR (2002) Application of DNA markers in soybean breeding. In: Gantt, S, Samac, D, Vance, C, Vanden Bosch, K and Young, N (editors) Proceedings of the First International Conference on Legume Genomics and Genetics: Translation to Crop Improvement. Minneapolis-St. Paul, Minnesota: University of Minnesota, pp. 55–58.Google Scholar
Brouwer, DJ and St. Clair, DA (2004) Fine mapping of three quantitative trait loci for late blight resistance in tomato using near isogenic lines and sub-NILs. Theoretical and Applied Genetics 108: 628–638.Google Scholar
Duan, YH, Wang, WX and Chang, RZ (2003) Efficient selection of genetic background using SSR markers in soybean. Journal of Plant Genetic Resources 4(1): 36–42.Google Scholar
Frary, A, Nesbitt, TC, Frary, A, et al. (2000) fw2.2: A quantitative trait locus key to the evolution of tomato fruit size. Science 289: 85–88.Google Scholar
Hospital, FC and Chevalet, PM (1992) Using markers in gene introgression breeding programes. Genetics 132: 1199–1210.Google Scholar
Li, JM, Thomson, M and McCouch, SR (2004) Fine mapping of a grain-weight quantitative trait locus in the pericentromeric region of rice chromosome 3. Genetics 168(4): 2187–2195.Google Scholar
Mackill, DJ and Bonman, JM (1992) Inheritance of blast resistance in near isogenic lines of rice. Phytopathology 82: 746–749.Google Scholar
McCouch, SR, Kochert, G, Yu, ZH, et al. (1988) Molecular mapping of rice chromosomes. Theoretical and Applied Genetics 76: 815–829.Google Scholar
McCouch, SR, Temnykh, S, Lukashova, A, Coburn, J, DeClerck, G, Cartinhour, S, Harrington, S and Thomson, M (2001) Microsatellite markers in rice: abundance, diversity, and applications. In: Khush, GS, Brar, DS and Hardy, B (editors) Rice Genetics IV. Proceedings of the 4th International Rice Genetics Symposium. New Delhi: Science Publ./Los Baños, Philippines: International Rice Research Institute, pp. 117–135.Google Scholar
Mu, P (2004) Correlation analysis and comparison of QTLs associated with drought resistance traits using DH and RIL populations derived from japonica upland and lowland rice cross. PhD Dissertation, China Agricultural University, Beijing, pp. 33–78.Google Scholar
Saito, K, Miura, K, Nagano, K, Hayano-Saito, Y, Araki, H and Kato, A (2001) Identification of two closely linked quantitative trait loci for cold tolerance on chromosome 4 of rice and their association with anther length. Theoretical and Applied Genetics 103: 862–868.Google Scholar
Saito, K, Hayano-Saito, Y, Miura, K, Maruyama-Funatsuki, W, Sato, Y and Kato, A (2004) Physical mapping and putative candidate gene identification of a quantitative trait locus Ctb1 for cold tolerance at the booting stage of rice. Theoretical and Applied Genetics 109: 515–522.Google Scholar
Shen, L, Courtois, B, McNally, KL, Robin, S and Li, Z (2001) Evaluation of near-isogenic lines of rice introgressed with QTLs for root depth through marked-aided selection. Theoretical and Applied Genetics 103: 75–83.Google Scholar
Tan, ZB, Zhang, Q, Zhu, LH and Wang, CL (1998) RFLP mapping of a rice bacterial blight resistance gene Xa-1. Hereditas 20(6): 30–33.Google Scholar
Tanksley, SD (1983) Molecular markers in plant breeding. Plant Molecular Biology Reporter 1: 3–8.Google Scholar
Tanksley, SD and Nelson, JC (1996) Advanced backcross QTL analysis: a method for the simultaneous discovery and transfer of valuable QTLs from unadapted germplasm into elite breeding lines. Theoretical and Applied Genetics 92: 191–203.Google Scholar
Temnykh, S, Park, WD and Ayres, N (2000) Mapping and genome organization of microsatellite sequences in rice (Oryza sativa L.). Theoretical and Applied Genetics 100: 697–712.Google Scholar
Wang, WM, Zhou, YL, Jiang, GH (2000) Fing mapping of the rice bacterial blight resistance gene Xa-4 and its co-segregation marker. Chinese Science Bulletin 45(19): 1779–1783.CrossRefGoogle Scholar
Xia, JH and Zheng, YL (2002) Molecular marker-assisted backcross breeding of maize Rf3 NIL and its efficient analysis. Acta Agronomica Sinica 28(3): 339–344.Google Scholar
Xu, SC, Wu, LR and Wan, AM (2004) Establishment of near isogenic lines resistant to wheat stripe rust using TaiChung29 as recurrent parent. Plant Protection 30(2): 19–22.Google Scholar
Yamamoto, T, Kuboki, Y, Lin, SY, Sasaki, T and Yano, M (1998) Fine mapping of quantitative trait loci Hd-1, Hd-2 and Hd-3, controlling heading date of rice, as single mendelian factors. Theoretical and Applied Genetics 97: 37–44.Google Scholar
Zhang, Q, Wang, CL, Zhao, KJ, et al. (2002) Development of near-isogenic line CBB23 with a new resistance gene to bacterial blight in rice and its application. Chinese Journal of Rice Science 16(3): 206–210.Google Scholar
Zhuge, Q, Zhang, B, Huang, MR and Wang, MX (2003) Progress in the selection of plant near isogenic lines and its application in the genetic improvement of forest trees. Forest Research 16(6): 754–759.Google Scholar