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Comparison of methylation level of genomes among different animal species and various tissues

Published online by Cambridge University Press:  15 June 2007

Tang Shao-Qing ,
Zhang Yuan ,
Xu Qing ,
Sun Dong-Xiao  and
Yu Ying
Tang Shao-Qing
Affiliation:
Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing 100094, China
Zhang Yuan*
Affiliation:
Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing 100094, China
Xu Qing
Affiliation:
Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing 100094, China
Sun Dong-Xiao
Affiliation:
Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing 100094, China
Yu Ying
Affiliation:
Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing 100094, China
*
*Corresponding author. E-mail: changy@cau.edu.cn
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Abstract

The methylation levels of genomes were compared in swine, cattle, sheep, rat, chicken and duck, using the methylation-sensitive amplification polymorphism technique (MSAP). The results showed that the methylation levels in genomes of the species investigated were mostly about 40–50% (except cattle); the methylation level varied in different species; the methylation pattern in various tissues of each species was specific; for the same species, the methylation level of the tissue genome was mostly higher than that of the blood genome; the difference of methylation level between birds and mammals was not significant, however mammals appeared to have a lower hemi-methylation frequency and higher full methylation frequency than birds.

Keywords

methylation leveldifferent animalsvarious tissuesmethylation-sensitive amplification polymorphism (MSAP)
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 4 , Issue 1 , April 2007 , pp. 75 - 79
Copyright
Copyright © China Agricultural University and Cambridge University Press 2007

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Footnotes

First published in Journal of Agricultural Biotechnology 2006, 14(4): 507–510

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