Hostname: page-component-7c8c6479df-7qhmt Total loading time: 0 Render date: 2024-03-29T10:06:45.422Z Has data issue: false hasContentIssue false

Characterization of genotypes and genetic relationships of cili (Rosa roxburghii) and its relatives using RAPD markers

Published online by Cambridge University Press:  12 February 2007

Wen Xiao-Peng
Affiliation:
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China Guizhou Key Laboratory of Agricultural Bioengineering, Guizhou University, Guiyang 550025, China
Deng Xiu-Xin*
Affiliation:
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China
*
*Corresponding author: Email: dxxwwlj@public.wh.hb.cn

Abstract

Cili (Rosa roxburghii Tratt), characterized by containing the highest vitamin C content among fruits and showing attractive senescence-retarding and cancer-preventing effects, has gained widespread interest. RAPD markers were applied to identify the seven genotypes of cili and to evaluate the genetic relationships within cili, as well as among its relatives. Sixteen arbitrary primers screened from 154 were adopted to analyse polymorphism in RAPD profiles for the 15 samples. A total of 137 RAPD bands ranging in size from 480 bp to 3.3 kb were obtained, among which 95 were polymorphic, covering 69.3% of the total bands obtained; and an average of 8.6 bands/primer was scored. The genotypes of cili and seedless cili could be identified efficiently by 14 genotype-specific bands, which were obtained from the polymorphic primers OPB-11, OPAF-16 and OPW-02. Additionally, using the unweighted pair group method with arithmetic mean, a dendrogram showing genetic relationships among the 15 samples was constructed based on cluster analysis of genetic distance. The possible origin of seedless cili and multiple-corolla cili is also discussed.

Type
Research Article
Copyright
Copyright © China Agricultural University and Cambridge University Press 2004

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bachmann, K (1992) Phenotypic similarity and genetic relationship among population of Microseris bigelovii (Asteraceae: Lactucese). Botanica Acta 105: 337342.CrossRefGoogle Scholar
Belaj, A, Satovic, Z and Rallo, L et al. . (2002) Genetic diversity and relationships in olive (Olea europaea L.) germplasm collections as determined by randomly amplified polymorphic DNA. Theoretical and Applied Genetics 105: 638644.Google Scholar
Emmarold, EM, Sinclair, HM and Mark, B (2001) Use of random amplified polymorphic DNA (RAPD) markers to reveal genetic diversity within and between populations of cashew ( Anacardium occidendale L.). Journal of Horticultural Science & Biotechnology 76: 375383.Google Scholar
Fagan, G (1988) Roses at the Cape Of Good Hope. Cape Town: Breestraat-Publikasies, 1988, pp. 210211Google Scholar
Fan, WG, Xia, GL and Luo, YC (1997) Utilization of Rosa roxburghii resources and its development strategy in Guizhou Province. Southwest China Journal of Agricultural Science 10: 109115. (in Chinese).Google Scholar
Federici, CT, Fang, DQ and Scora, RW (1998) Phylogenetic relationships within the genus Citrus(Rutaceae) and related genera as revealed by RFLP and RAPD analysis. Theoretical and Applied Genetics 96: 812822.CrossRefGoogle Scholar
Ji, XB and Li, SJ (1998) A morphology and anatomy study on four species of cili in Guizhou. Journal of Mountain Agriculture and Biology 1: 2833. (in Chinese with English abstract).Google Scholar
Luo SL, He, PC Zhou, P et al. . (2001) Construction of molecular linkage map in grape using RAPD markers and F1 population of interspecific cross. Acta Horticulturae Sinica 28: 6870. (in Chinese with English abstract).Google Scholar
Ma, YX, Zhu, Y and Wang, CF et al. . (1997) The aging retarding effect of ‘Long-Life CiLi’. Mechanisms of Ageing and Development 96: 171189.Google ScholarPubMed
Maria, TD and Maria, AP (2000) Identification of Feijoa sellowiana Berg samples by RAPD markers. Scientia Horticulturae 86: 279290.Google Scholar
Nei, M and Li, WH (1979) Mathematical modal for studying genetic variation in terms of restriction endonucleases. Proceedings of the National Academy of Sciences of the USA 76: 52695273.Google Scholar
Papa, R, Attene, G and Barcaccia, G (1998) Genetic diversity in landrace population of Hordeum vulgare L. from Sardinia, Italy, as revealed by RAPDs, isozymes and morphological traits. Plant Breeding 117: 523530.Google Scholar
Persson, HA, Runpunen, K and Mollerstedt, LK (2000) Identification of culinary rhubarb (Rheum spp.) cultivars using morphological characterization and RAPD markers. Journal of Horticultural Science & Biotechnology 75: 684689.CrossRefGoogle Scholar
Shi, JK, Xiang, XX and Gao, XF et al. . (1991) Progress in the study of cili in China. Journal of Guizhou Agricultural College 10: 8894. (in Chinese).Google Scholar
Vilanova, S, Badenes, ML, Martiner-Calvo, J et al. . (2001) Analysis of loquat (Eriobotrya japonica, Lindl.) by RAPD molecular markers. Euphytica 121: 2529.Google Scholar
Wang, Y, Chen, J and Lu, J (1999) Randomly amplified polymorphic DNA analysis of Vitis species and Florida bunch grapes. Scientia Horticulturae 82: 8594.CrossRefGoogle Scholar
Wen, XP and Deng, XX (2002) The extraction of genomic DNA from five species of Rosa. Seed 126: 1821. (in Chinese).Google Scholar
Wiggers, R J, West, JG and Taylor, J (1997) Conidial germination and infection by Diplocarpon rosae on susceptible and resistant rose species. Mycologia 9: 103108.CrossRefGoogle Scholar
Williams, JGK, Kubelik, AR, Livak, KJ et al. . (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18: 65316535.Google Scholar
Xiang, XH, Liu, JP, Fan, WG (1987) The germplasm and its utilization of Rosa roxburghii Tratt in Guizhou. Water Soil Protection Sinica 7: 3435. (in Chinese with English abstract).Google Scholar
Yu, T (1985) Flora Reipublicae Popularis Sinicae Tomus 37. Beijing: Science Press, pp. 452453 (in Chinese).Google Scholar