Hostname: page-component-76fb5796d-dfsvx Total loading time: 0 Render date: 2024-04-25T12:56:37.551Z Has data issue: false hasContentIssue false
  • English
  • Français

Auxin regulation in the interaction between Rice stripe virus and rice

Published online by Cambridge University Press:  24 April 2009

Yang Jin-Guang ,
Wang Wen-Ting ,
Ding Xin-Lun ,
Guo Li-Juan ,
Fang Zhen-Xing ,
Xie Li-Yan ,
Lin Qi-Ying ,
Wu Zu-Jian  and
Xie Lian-Hui
Yang Jin-Guang
Affiliation:
Key Laboratory of Plant Virology of Fujian Province, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, China Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, Shandong 266101, China
Wang Wen-Ting
Affiliation:
Key Laboratory of Plant Virology of Fujian Province, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Ding Xin-Lun
Affiliation:
Key Laboratory of Plant Virology of Fujian Province, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Guo Li-Juan
Affiliation:
Key Laboratory of Plant Virology of Fujian Province, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Fang Zhen-Xing
Affiliation:
Key Laboratory of Plant Virology of Fujian Province, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Xie Li-Yan
Affiliation:
Key Laboratory of Plant Virology of Fujian Province, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Lin Qi-Ying
Affiliation:
Key Laboratory of Plant Virology of Fujian Province, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Wu Zu-Jian*
Affiliation:
Key Laboratory of Plant Virology of Fujian Province, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Xie Lian-Hui*
Affiliation:
Key Laboratory of Plant Virology of Fujian Province, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
*
*Corresponding authors. E-mail: wuzujian@126.com or fjxlh@126.com
*Corresponding authors. E-mail: wuzujian@126.com or fjxlh@126.com
Get access Rights & Permissions [Opens in a new window]

Abstract

The expression of the YUCAA1 gene and the amount of endogenous indole acetic acid (IAA) in rice (Oryza sativa subsp. japonica) plants and rice suspension cells infected by Rice stripe virus (RSV) were investigated by real-time reverse transcriptase–polymerase chain reaction (RT-PCR) and high-performance liquid chromatography, respectively. The results showed that the expression of the YUCAA1 gene and the amount of endogenous IAA increased at various times (16, 32, 48 and 64 h) after infection of rice suspension cells by RSV. In rice plants infected with RSV, the expression of the YUCAA1 gene and the amount of endogenous IAA increased, in comparison with healthy rice plants, at 4–8 days after infection, and decreased at 12 and 16 days. These results indicated that RSV infection could regulate auxin biosynthesis in rice. Additionally, the expression of the RSV gene CP increased 2.9 times in rice plants after they were treated with a KPSC buffer to deplete the endogenous auxins, and decreased 45% after treatment with 30 μmol/l IAA. All of these results suggest that auxin may play a role in RSV replication in rice plants.

Keywords

auxinriceRice stripe virus (RSV)RT-PCR
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 6 , Issue 1 , April 2009 , pp. 27 - 33
Copyright
Copyright © China Agricultural University 2009

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Abel, S and Theologis, A (1996) Early genes and auxin action. Plant Physiology 111(1): 9–17.CrossRefGoogle ScholarPubMed
Bartsch, M, Gobbato, E, Bednarek, P, et al. (2006) Salicylic acid-independent ENHANCED DISEASE SUSCEPTIBILITY 1 signaling in Arabidopsis immunity and cell death is regulated by the monooxygenase FMO1 and the nudix hydrolase NUDT7. Plant Cell 18(4): 1038–1051.CrossRefGoogle Scholar
Hamamatsu, C and Ishihama, A (1994) Ambisense RNA genome of Rice stripe virus. Uirusu 44(1): 19–25.CrossRefGoogle ScholarPubMed
Jain, M, Kaur, N, Tyagi, AK and Khurana, JP (2006a) The auxin-responsive GH3 gene family in rice (Oryza sativa). Functional and Integrative Genomics 6(1): 36–46.CrossRefGoogle ScholarPubMed
Jain, M, Nijhawan, A, Tyagi, AK and Khurana, JP (2006b) Validation of housekeeping genes as internal control for studying gene expression in rice by quantitative real-time PCR. Biochemical and Biophysical Research Communications 345(2): 646–651.CrossRefGoogle ScholarPubMed
Jain, M, Kaur, N, Garg, R, Thakur, JK, Tyagi, AK and Khurana, JP (2006c) Structure and expression analysis of early auxin-responsive Aux/IAA gene family in rice (Oryza sativa). Functional and Integrative Genomics 6(1): 47–59.CrossRefGoogle ScholarPubMed
Jain, M, Tyagi, AK and Khurana, JP (2006d) Genome-wide analysis, evolutionary expansion, and expression of early auxin-responsive SAUR gene family in rice (Oryza sativa). Genomics 88(3): 360–371.CrossRefGoogle ScholarPubMed
Kelen, M, Cubuk Demiralay, E, Sen, S and Ozkan, G (2004) Separation of abscisic acid, indole-3-acetic acid, gibberellic acid in 99 R (Vitis berlandieri×Vitis rupestris) and rose oil (Rosa damascena Mill.) by reversed phase liquid chromatography. Turkish Journal of Chemistry 28: 603–610.Google Scholar
Lorenzo, O, Piqueras, R, Sanchez-Serrano, JJ and Solano, R (2003) ETHYLENE RESPONSE FACTOR 1 integrates signals from ethylene and jasmonate pathways in plant defense. Plant Cell 15(1): 165–178.CrossRefGoogle ScholarPubMed
Marshall, OJ (2004) PerlPrimer: Cross-platform, graphical primer design for standard, bisulphite and real-time PCR. Bioinformatics 20(15): 2471–2472.CrossRefGoogle ScholarPubMed
Navarro, L, Dunoyer, P, Jay, F, et al. (2006) A plant miRNA contributes to antibacterial resistance by repressing auxin signaling. Science 312(5772): 436–439.CrossRefGoogle ScholarPubMed
Padmanabhan, MS, Goregaoker, SP, Golem, S, Shiferaw, H and Culver, JN (2005) Interaction of the Tobacco mosaic virus replicase protein with the Aux/IAA protein PAP1/IAA26 is associated with disease development. Journal of Virology 79(4): 2549–2558.CrossRefGoogle ScholarPubMed
Shirasu, K, Nakajima, H, Rajasekhar, VK, Dixon, RA and Lamb, C (1997) Salicylic acid potentiates an agonist-dependent gain control that amplifies pathogen signals in the activation of defense mechanisms. Plant Cell 9(2): 261–270.Google ScholarPubMed
Takahashi, M, Toriyama, S, Hamamatsu, C and Ishihama, A (1993) Nucleotide sequence and possible ambisense coding strategy of rice stripe virus-RNA segment 2. Journal of General Virology 74: 769–773.CrossRefGoogle ScholarPubMed
Toriyama, S (1982) Characterization of Rice stripe virus: a heavy component carrying infectivity. Journal of General Virology 61: 187–195.CrossRefGoogle Scholar
Toriyama, S and Tomaru, K (1995) Genus Tenuivirus. In Murphy, FA, Fauquet, CM, Bishop, DHL, et al. Virus Taxonomy, Classification and Nomenclature of Viruses, Sixth Report of the International Committee on Taxonomy of Viruses. Wien: Springer.Google Scholar
Toriyama, S, Takahashi, M, Sano, Y, Shimizu, T and Ishihama, A (1994) Nucleotide sequence of RNA1, the largest genomic segment of Rice stripe virus, the prototype of the tenuiviruses. Journal of General Virology 75(12): 3569–3579.CrossRefGoogle Scholar
Wei, TY (2003) Molecular Population Genetics of Rice Stripe Virus. Fujian Agricultural and Forestry University (in Chinese).Google ScholarPubMed
Yalpani, N, Silverman, P, Wilson, MA, Kleier, DA and Raskin, L (1991) Salicylic acid is a systemic signal and an inducer of pathogenesis-related proteins in virus-infected tobacco. Plant Cell 3(8): 809–818.Google Scholar
Yang, WD, Wang, XH, Wang, SY, Yie, Y and Tien, P (1996) Infection and replication of a planthopper transmitted virus: Rice stripe virus in rice protoplasts. Journal of Virological Method 59(1–2): 57–60.CrossRefGoogle ScholarPubMed
Zhang, J, Peng, Y and Guo, Z (2008) Constitutive expression of pathogen-inducible OsWRKY31 enhances disease resistance and affects root growth and auxin response in transgenic rice plants. Cell Research 18(4): 508–521.CrossRefGoogle ScholarPubMed
Zhu, Y, Hayakawa, T, Toriyama, S and Takahashi, M (1991) Complete nucleotide sequence of RNA3 of Rice stripe virus: An ambisense coding strategy. Journal of General Virology 72(4): 763–767.CrossRefGoogle Scholar
Zhu, Y, Hayakawa, T and Toriyama, S (1992) Complete nucleotide sequence of RNA4 of Rice stripe virus isolate T and comparison with another isolate and with Maize stripe virus. Journal of General Virology 73(5): 1309–1312.CrossRefGoogle Scholar