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Isolation and analysis of sox9 gene derived from yellow catfish Pelteobagrus fulvidraco

Published online by Cambridge University Press:  12 February 2007

Yu Ju-Hua
Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi Jiangsu 214081, China
Li Jian-Lin
Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi Jiangsu 214081, China
Cao Li-Ping
Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi Jiangsu 214081, China
Wu Ting-Ting*
Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi Jiangsu 214081, China
Yang Hong
Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi Jiangsu 214081, China
*Corresponding author. Email:


Sox9 is one of the important genes related to sex differentiation and determination. Two partial cDNAs encoding sox9 were derived from brain, testis and ovary of yellow catfish Pelteobagrus fulvidraco using reverse transcriptase-polymerase chain reaction (RT-PCR). Sequence analysis revealed that the ovarian sox9a2 was different from sox9a1 derived from brain and testis. The identity rate of cDNA was 77%, that of amino acids was 84%, and the different nucleotides were distributed in different sites all over the cDNA. This finding confirmed that there were transcripts from two genes. Based on the homology of the amino-acid sequences for sox9 of other animals, the similarity for sox9a1 from yellow catfish and sox9 of other animals averaged 86%. The similarity for sox9a2 reached a mean of 83%. The phylogenetic analysis revealed yellow catfish sox9a1 and sox9a2 were clustered into the fish branch. And yellow catfish sox9a2 was different from the other fish sox9. There was no significant branch including all sox9 derived from testis and brain or including all ovarian sox9. The genetic distance was the same with the results of the traditional taxonomy. RT-PCR analysis indicated that sox9a1 was expressed in male and female yellow catfish forebrain as well as in gonads, whereas sox9a2 was only expressed in ovaries. The function of sox9a2 in female sex formation and ovary development needs to be studied in depth.

Research Article
Copyright © China Agricultural University and Cambridge University Press 2006

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Bull, JJ (1983) Evolution of sex determination mechanisms. Menlo Park, California, USA: Benjamin/Cummings.Google Scholar
Cai, YZ, Cai, YQ and He, CR (2003) The study on the biology of yellow catfish (Pelteobagrus fulvidraco). Available at Scholar
Chang, ZJ, Zhou, RJ and Yu, QX (2000) The conserved region sequence analysis of PdSox9 in Paramisgurnus dabryanus. Acta Genetica Sinica 27(2): 121126.Google ScholarPubMed
Chiang, EF, Tong, SK, Yan, YL, et al. (2001) Two sox9 genes on duplicated zebrafish chromosomes: expression of similar transcription activators in distinct sites. Developmental Biology 231: 149163.CrossRefGoogle ScholarPubMed
Clarkson, MJ and Harley, VR (2002) Sex with two SOX on: SRY and SOX9 in testis development. Trends in Endocrinology and Metabolism 13: 106111.CrossRefGoogle ScholarPubMed
Conover, DO and Kynard, BE (1981) Environmental sex determination: interaction of temperature and genotype in a fish. Science 213: 577579.CrossRefGoogle ScholarPubMed
Coriat, AME and Valleley, WJM (1994) Chromosomal and temperature dependent sex determination: the search for a conserved mechanism. Journal of Experimental Zoology 270: 11121116.Google ScholarPubMed
Donaldson, EM and Hunter, GA (1982) Sex control in fish with particular reference to salmonids. Canadian Journal of Fisheries and Aquatic Sciences 39: 99110.CrossRefGoogle Scholar
Hunter, GA and Donaldson, EM (1983) Hormonal sex control and its application to fish culture. In: Hoar, WS, Randall, DJ and Donaldson, EM (editors) Fish Physiology, IXB. New York, NY: Academic Press, pp. 223303.Google Scholar
Koopman, P, Munsterberg, A and Capel, B (1990) Expression of a candidate sex determining gene during mouse testis differentiation. Nature 348: 450452.CrossRefGoogle ScholarPubMed
Nie, LW, Shan, XN, Wang, M, Guo, CW and Lu, XX (2001) RT-PCR analysis on Sox gene of seven tissues from the Chinese soft-shelled turtle (Pelosiscus sinensis). Acta Zoological Sinica 47(6): 718720.Google Scholar
Rose, TM, Schultz, ER, Henikoff, JG, Pietrokovski, S, McCallum, CM and Henikoff, S (1998) Consensus-degenerate hybrid oligonucleotide primers for amplification of distantly-related sequences. Nucleic Acids Research 26(7): 16281635.CrossRefGoogle ScholarPubMed
Rodriguez-Mari, A, Yan, YL, Bremiller, RA, Wilson, C, Canestro, C and Postlethwait, JH (2005) Characterization and expression pattern of zebrafish Anti-Mullerian hormone (Amh) relative to sox 9a, sox 9b, and cyp19a1a, during gonad development. Gene Expression Patterns 5: 655667.CrossRefGoogle Scholar
Swofford, DL (1998) PAUP: Phylogenetic Analysis Using Parsimony and other methods. Version 4. Sunderland, MA: Sinauer Associates.Google Scholar
Thompson, JD, Higgins, DG and Gibson, TJ (1994) Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22: 46734680.CrossRefGoogle ScholarPubMed
Vidal, VPI, Chaboissier, MC, de Rooij, DG and Schedl, A (2001) Sox9 induces testis development in XX transgenic mice. Nature Genetics 28: 216217.CrossRefGoogle ScholarPubMed
Wright, EM, Snopek, B and Koopman, P (1993) Seven new numbers of the Sox gene family expressed during mouse development. Nucleic Acids Research 21(3): 744.CrossRefGoogle Scholar
Yamamoto, T (1969) Sex differentiation. In: Hoar, WS, Randall, DJ and Donaldson, EM (editors). Fish Physiology III. New York, NY: Academic Press, pp. 117175.Google Scholar
Zhou, R, Liu, L, Guo, Y, Yu, H, Cheng, H, Huang, X, et al. (2003) Similar gene structure of two Sox9a genes and their expression patterns during gonadal differentiation in a teleost fish, rice field eel (Monopterus albus). Molecular Reproduction Development 66(3): 211217.CrossRefGoogle Scholar