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In vitro differentiation of chicken spermatogonial stem cells into adipocytes

Published online by Cambridge University Press:  03 March 2009

Yu Fei
Affiliation:
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Ge Jian-Hui
Affiliation:
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Ni Li-Gang
Affiliation:
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
He Xian-Hong
Affiliation:
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Xu Qi
Affiliation:
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Li Bi-Chun*
Affiliation:
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China Institute of Animal Husbandry and Veterinary, Jiangsu Academy of Agricultural Science, Nanjing 210014, China
*
*Corresponding author. E-mail: yubcli@yzu.edu.cn

Abstract

Spermatogonial stem cells (SSCs), which were isolated from chicken (Gallus gallus) embryo testes 16 days after laying, were cultured, subcultured, and induced into adipocytes in vitro. The differentiated cells were identified by oil red-O staining. Dexamethasone, insulin and 3-isobutyl-1-methylxanthine (IBMX) were tested for their induction potential. About 7–21 days after induction, SSCs differentiated into adipocytes, and the resulting adipocytes strongly expressed peroxisome proliferation activation receptor-γ (PPAR-γ). The assay outcome showed that an optimal treatment consisted of dexamethasone, insulin and IBMX application for 3 days and insulin for 1 day (3 cycles), then insulin for 21 days. The differentiation ratio was 85%, better than the combined use of dexamethasone, insulin and IBMX (P<0.01). However, the combination of the three derivatives triggered a stronger induction than any of them used alone (P<0.01). This study has demonstrated the potential of chicken embryonic SSCs to differentiate in vitro into adipocytes.

Type
Research Papers
Copyright
Copyright © China Agricultural University 2008

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Footnotes

First published in Journal of Agricultural Biotechnology 2008, 16(3): 450–455

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