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Cloning and expression analysis of porcine small adipocyte factor 1 (SMAF1) gene

Published online by Cambridge University Press:  02 August 2007

Yang Hong-Wen
Affiliation:
Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agriculture University, Wuhan 430070, China
Zheng Rong
Affiliation:
Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agriculture University, Wuhan 430070, China
Li Feng-E
Affiliation:
Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agriculture University, Wuhan 430070, China
Jiang Si-Wen*
Affiliation:
Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agriculture University, Wuhan 430070, China
*
*Corresponding author. E-mail: jiangsiwen@mail.hzau.edu.cn

Abstract

cDNA of the porcine small adipocyte factor 1 (SMAF1) gene was amplified by reverse transcriptase-polymerase chain reaction (RT-PCR) with degenerate primers designed according to the conserved sequences between human and mouse genes. The cDNA (GenBank accession No. DQ191892) containing a complete encoding region was 256 bp in length, sharing 86 and 78% identity with that of human and mouse, respectively. Comparison of the deduced amino acid sequence between porcine SMAF1 and the human, mouse, cattle and rat protein showed that the amino acid similarity was 81, 67, 84 and 70%, respectively. The results of semi-quantitative RT-PCR showed that the porcine gene was expressed abundantly in adipose tissue, and at a significantly lower level in lean-type pigs (Large White pigs) than in lard-type pigs (Meishan pigs) at the age of 4 months (P<0.05). The results suggest that the porcine SMAF1 gene may have similar functions as in other species, that is, it may regulate adipogenesis and/or adipocyte function.

Type
Research Article
Copyright
Copyright © China Agricultural University and Cambridge University Press 2004

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Footnotes

First published in Journal of Agricultural Biotechnology 2007, 15(1): 11–14

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