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Cloning and characteristics of a novel gene HbUEP from latex in Hevea brasiliensis

Published online by Cambridge University Press:  01 October 2008

Yang Yun
Affiliation:
Institute of Tropical Biosciences and Biotechnology, CATAS, Haikou 571101, China Institute of Medicinal Plant Development, Hainan Branch, Wanning 571500, China
Zhang Zhi-Li*
Affiliation:
Institute of Tropical Biosciences and Biotechnology, CATAS, Haikou 571101, China
Liu Kuan-Can
Affiliation:
Institute of Tropical Biosciences and Biotechnology, CATAS, Haikou 571101, China
Li Wei-Guo
Affiliation:
Institute of Rubber Research, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China
Su Huo-Sheng
Affiliation:
Institute of Tropical Biosciences and Biotechnology, CATAS, Haikou 571101, China
*
*Corresponding author. E-mail: zzl_catas@hotmail.com

Abstract

HbUEP, an ubiquitin extension protein gene from latex of the rubber tree (Hevea brasiliensis) was cloned and sequenced using a differentially ethphon-induced expressed cDNA subtraction library. The cDNA had 771 bp nucleotides, comprising a 226 bp 3′ untranslated region (UTR), 77 bp 5′UTR and a 468 bp open reading frame encoding a 156 amino acid peptide. Southern blotting analysis showed that this gene was a low copy number gene in the H. brasiliensis genome. Within 24 h after application of ethphon, the gene was expressed weakly in both control and latex sampled at 6 h, and strongly in latex sampled at 12 h, showing that this gene expression could be regulated by ethphon. Ethphon could increase the latex yield in H. brasiliensis. It is suggested that the HbUEP gene may be involved in the regulation of ethphon-induced high latex yield in H. brasiliensis.

Type
Research Papers
Copyright
Copyright © China Agricultural University 2008

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Footnotes

First published in Journal of Agricultural Biotechnology 2008, 16(2): 305–308

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