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Analysis of microsatellite DNA markers of OarHH35 and BMS2508 in four goat breeds

Published online by Cambridge University Press:  20 March 2007

Ouyang Xu-Xiang*
Affiliation:
College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China Department of Animal Science and Technology, Hunan Biological and Electromechanical Polytechnic, Changsha 410127, China
Si Qi-Shun
Affiliation:
College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China
Huang Sheng-Qiang
Affiliation:
College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China
Deng Zao-Fu
Affiliation:
Department of Animal Science and Technology, Hunan Biological and Electromechanical Polytechnic, Changsha 410127, China
Liu He -Xiang
Affiliation:
Department of Animal Science and Technology, Hunan Biological and Electromechanical Polytechnic, Changsha 410127, China
He De-Shi
Affiliation:
Department of Animal Science and Technology, Hunan Biological and Electromechanical Polytechnic, Changsha 410127, China
Tan Sheng-Guo
Affiliation:
Department of Animal Science and Technology, Hunan Biological and Electromechanical Polytechnic, Changsha 410127, China
Hu Shu-Guang
Affiliation:
Department of Animal Science and Technology, Hunan Biological and Electromechanical Polytechnic, Changsha 410127, China
*
*Corresponding author. E-mail: ouwxu@163.com

Abstract

Two microsatellite markers OarHH35 and BMS2508, closely associated with high levels of reproduction in sheep, were analysed for polymorphisms in Xiangdong black goat, Nanjiang brown goat, Guizhou black goat and Boer goat. The number of alleles for OarHH35 was 11, 8, 7 and 10, respectively, in the four goat breeds. That for BMS2508 was 9, 5, 6 and 7. The polymorphic information content (PIC) values for OarHH35/BMS2508 in the above-mentioned goats were respectively 0.8294/0.8047, 0.8399/0.6894, 0.7985/0.6910 and 0.8582/0.7688. By analysing the dominant effect of the two microsatellite markers on litter size of Xiangdong black goat, it was shown that microsatellite markers BMS2508 and OarHH35 significantly influenced (P<0.05) litter size of goats. At the OarHH35 locus, the least squares means (LSM) of genotype 135/135 bp was the highest (3.67 number born/litter). Moreover, LSM of genotypes 123/135 bp and 125/125 bp also reached 3.00 kids born/litter, indicating that alleles 135 bp and 125 bp exerted a significant positive effect on the litter size in Xiangdong black goat. At the BMS2508 locus, LSM of genotypes 132/145 bp and 93/132 bp for litter size was 4.00 and 3.25 kids born/litter respectively, but that of genotype 122/122 bp for litter size was only 1.00 kid/litter, so alleles 145 bp and 93 bp had significant positive effects on the litter size of Xiangdong black goat, and the allele 122 bp had a significantly negative effect.

Type
Research Article
Copyright
China Agricultural University and Cambridge University Press 2006

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References

Botstein, D, White, RL, Skolnick, M and Davis, RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American Journal of Human Biology 32: 314331.Google ScholarPubMed
Chu, MX, Cheng, JH and Guo, W (2001) Preliminary studies of microsatellite markers OarAE101 and BM1329 in five sheep breeds. Acta Genetica Sinica 28: 510517.Google ScholarPubMed
Chu, MX, Wang, JZ, Wang, AG, Li, N and Fu, JL (2002) Genetic polymorphisms of five microsatellite loci in small Tail Han sheep. Acta Genetica Sinica 29: 502506.Google ScholarPubMed
Luo, WY, Hu, J and Li, XF (2003) The evolution and application of microsatellites. Hereditas 25(5): 615619.Google ScholarPubMed
Ouyang, XX, Huang, SQ, Si, QS, et al. (2004) Cloning and sequence analysis of follicle stimulating hormone receptor gene in Chinese Xiangdong black goat. Journal of Hunan Agricultural University 30: 462465.Google Scholar
Shi, QS (1999) The progress of major genes research of livestock and poultry. Journal of Hunan Agricultural University 25: 8186.Google Scholar
Souza, CJ, MacDougall, C, Campbell, BK, McNeilly, AS, Baird, DT (2001) The Booroola (FecB) phenotype is associated with a mutation in the bone morphogenetic receptor type 1 B (BMPR1B) gene. Journal of Endocrinology 169: R16.CrossRefGoogle ScholarPubMed
Zhang, LJ, Yang, CS, Chen, H, Yan, HX, Luo, J and Zhang, BJ (2005) Application of 10 microsatellite loci to line identification of white egg-type chickens. Journal of Agricultural Biotechnology 13: 7276.Google Scholar
Zhang, YJ, Zhao, YZ, Liu, YQ, Li, Y, Sun, SH and Li, F (2003) Studies on genetic polymorphism using microsatellite markers OarAE101 and MCM38 in three goat breeds. Grass-feeding Livestock 2: 2426.Google Scholar