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A Method for Measurement of Arterial Concentration of Cerebral Blood Flow Tracer for Autoradiographic Experiments

Published online by Cambridge University Press:  18 September 2015

Devidas Menon
Affiliation:
Montreal Neurological Institute and Department of Neurology and Neurosurgery, McGill University, Montreal
Mirko Diksic*
Affiliation:
Montreal Neurological Institute and Department of Neurology and Neurosurgery, McGill University, Montreal
Ernst Meyer
Affiliation:
Montreal Neurological Institute and Department of Neurology and Neurosurgery, McGill University, Montreal
Kazuhiro Sako
Affiliation:
Montreal Neurological Institute and Department of Neurology and Neurosurgery, McGill University, Montreal
Y. Lucas Yamamoto
Affiliation:
Montreal Neurological Institute and Department of Neurology and Neurosurgery, McGill University, Montreal
*Corresponding
Medical Cyclotron Unit, Montreal Neurological Institute, 3801 University Street, Montreal, Quebec, Canada H3A 2B4
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Abstract:

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A new method for continuous monitoring of the concentration of 131I-labelled iodoantipy rine during an autoradiographic local cerebral blood flow (LCBF) experiment is described. During infusion of iodoantipy rine, radioactivity in an external femoral arteriovenous loop is monitored by a scintillation detector. Data is collected with a variable time base, and the arterial activity curve displayed visually in its entirety. The main advantage of this method is that there is no blood loss. It will therefore be of particular importance in dual-label autoradiography. This method can also be used for other gamma-emitting blood flow tracers, e.g. 123I-iodoantipyrine.

Type
Original Articles
Copyright
Copyright © Canadian Neurological Sciences Federation 1984

References

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