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A STANDARDIZED BIOASSAY FOR LARVICIDAL ACTIVITY OF BACILLUS THURINGIENSIS IN SUNFLOWER MOTH HOMOEOSOMA ELECTELLUM (LEPIDOPTERA: PHYCITIDAE)

Published online by Cambridge University Press:  31 May 2012

R. G. Lidstone ,
D. W. Goerzen  and
G. G. Khachatourians
R. G. Lidstone
Affiliation:
Bioinsecticide Laboratory, Department of Applied Microbiology and Food Science, University of Saskatchewan, Saskatoon S7N 0W0
D. W. Goerzen
Affiliation:
Bioinsecticide Laboratory, Department of Applied Microbiology and Food Science, University of Saskatchewan, Saskatoon S7N 0W0
G. G. Khachatourians
Affiliation:
Bioinsecticide Laboratory, Department of Applied Microbiology and Food Science, University of Saskatchewan, Saskatoon S7N 0W0
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Abstract

A standard test for the larvicidal activity of Bacillus thuringiensis (B.t.) against the larvae of the sunflower moth Homoeosoma electellum (Hulst) has been developed. Bioassay parameters investigated include diet preparation, concentration of B.t., effect of formaldehyde, and method of pathogen incorporation in diet. The LC50 for seconded third-instar larvae is 1.24 μg of Dipel® WP ml−1 or 19.8 IU B.t. ml−1 pathogen-incorporated diet. Layering of a pathogen-suspension upon the surface of the diet was not a reliable bioassay technique for H. electellum. The addition of formaldehyde in the diet reduced the slope of the dose–mortality curve but did not change the LC50.

Résumé

Un test standard d'activité larvicide de Bacillus thuringiensis (B.t.) contre les larves de pyrale de tournesol, Homoeosoma electellum (Hulst) a été développé. Les paramètres suivants de bioassay on été exploré : préparation de l'aliment; concentration de B.t.; effet de la formaldehyde; le procédé pour incorporer le pathogène dans l'aliment. Le LC50 pour les larves du deuxième et troisième instar est 1.24 μg/ml Dipel® ou 19.8 IU/ml B.t. pathogène incorporé dans l'aliment. Pour la technique de bioassay, une couche de nés pension pathogène sur la surface de l'aliment ne s'est pas avère satisfaisant. Dans l'aliment, l'addition de la formaldehyde a réduit la pente de la courbe de dose-mortalité, mais n'a pas changé le LC50.

Type
Articles
Information
The Canadian Entomologist , Volume 117 , Issue 1 , January 1985 , pp. 15 - 22
Copyright
Copyright © Entomological Society of Canada 1985

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References

Abbott, W. S. 1925. A method of computing the effectiveness of an insecticide. J. econ. Ent. 18: 265–267.Google Scholar
Angus, T. A. 1956. Extraction, purification and properties of Bacillus sotto toxin. Can. J. Microbiol. 2: 416–426.CrossRefGoogle ScholarPubMed
Arthur, A. P. 1978. The occurrence, life history, courtship, and mating behaviour of the sunflower moth, Homoeosoma electellum (Lepidoptera: Phycitidae), in the Canadian prairie provinces. Can. Ent. 110: 913–916.CrossRefGoogle Scholar
Beegle, C. C., Lewis, L. C., Martinez, R. E., and Martinez, A. J.. 1981. Interaction of larval age and antibiotic on the susceptibility of three insect species to Bacillus thuringiensis. J. invert. Path. 37: 143–153.CrossRefGoogle Scholar
Burges, H. D. (Ed.). 1981. Microbial Control of Pests and Plant Diseases 1970–1980. Academic Press, London. 949 pp.Google Scholar
Burges, H. D. and Thomson, E. M.. 1971. Standardization and Assay of Microbial Insecticides. In Burges, H. D. and Hussey, N. W. (Eds.), Microbial Control of Insects and Mites. Academic Press, NY. 861 pp.Google Scholar
Chippendale, G. M. and Kikukawa, S.. 1983. Effect of day length and temperature on the larval diapause of the sunflower moth, Homoeosoma electellum. J. Insect Physiol. 29(8): 643–649.CrossRefGoogle Scholar
Finney, D. J. 1971. Probit Analysis. Cambridge University Press. 333 pp.Google Scholar
Grisdale, D. 1970. A method of computing the effectiveness of an insecticide. J. econ. Ent. 102: 1111–1117.Google Scholar
Helms, J. J. and Raun, E. S.. 1971. Perennial laboratory culture of disease free insects. In Burges, H. D. and Hussey, N. W. (Eds.), Microbial Control of Insects and Mites. Academic Press, NY. 861 pp.Google Scholar
Ignoffo, C. M., Garcia, C., and Couch, T. C.. 1977. Effect of antibiotics on the insecticidal activity of Bacillus thuringiensis. J. invert. Path. 30: 277–278.CrossRefGoogle Scholar
McVay, J. R., Gudauskas, R. T., and Harper, J. T.. 1977. Effects of Bacillus thuringiensis nuclear polyhedrosis virus mixtures on Trichoplusia ni larvae. J. invert. Path. 29: 367–372.Google Scholar
Morris, O. N. and Moore, A.. 1983. Relative potencies of 50 isolates of Bacillus thuringiensis for larvae of the spruce budworm, Choristoneura fumiferana (Lepidoptera: Tortricidae). Can. Ent. 115: 815–822.Google Scholar
Nishiitsutsuji-Uwo, J. and Endo, Y.. 1980. Mode of action of Bacillus thuringiensis -endotoxin: relative roles of spores and crystals in toxicity to Pieris, Lymantria and Ephestia larvae. Appl. Ent. Zool. 15(4): 416–424.Google Scholar
Shorey, H. H. and Hale, R. L.. 1965. Mass-rearing of the larvae of nine noctuid species on a simple artificial medium. J. econ. Ent. 58: 522–524.CrossRefGoogle Scholar