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QUANTITATIVE STUDIES OF HYDROLYTIC ENZYME ACTIVITY IN THE SALIVARY GLAND OF CHIRONOMUS TENTANS (DIPTERA: CHIRONOMIDAE) DURING METAMORPHOSIS1

Published online by Cambridge University Press:  31 May 2012

Hans Laufer  and
Ki Ssu Schin
Hans Laufer
Affiliation:
Biological Sciences Group, University of Connecticut, Storrs and Marine Biological Laboratory, Woods Hole, Massachusetts
Ki Ssu Schin
Affiliation:
Biological Sciences Group, University of Connecticut, Storrs and Marine Biological Laboratory, Woods Hole, Massachusetts
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Abstract

Changes in the hydrolytic enzymes, ribonuclease and acid phosphatase were investigated in the salivary gland of Chironomus tentans since these lysosomal enzymes may participate in hormonally stimulated tissue breakdown. Quantitative assays revealed 9- and 12-fold increases in the specific activity of these enzymes during pupation while the protein content of the gland was decreasing. These increases cannot be accounted for by decreased protein but may represent an activation, accumulation, or synthesis which seems to be important in gland breakdown at metamorphosis.

Type
Articles
Information
The Canadian Entomologist , Volume 103 , Issue 3 , March 1971 , pp. 454 - 457
Copyright
Copyright © Entomological Society of Canada 1971

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References

Ambellan, E. and Hollander, V. P.. 1966. A simplified assay for RNase activity in crude tissue extracts. Analyt. Biochem. 17: 474–484.CrossRefGoogle ScholarPubMed
Andersch, M. A. and Szczypinski, A. J.. 1947. Use of p-Nitrophenyl phosphate as the substrate in determination of serum acid phosphatase. Am. J. clin. Path. 17: 571–574.CrossRefGoogle ScholarPubMed
Clever, U. and Karlson, P.. 1960. Induktion von Puff-veränderungen in den Speicheldrüsenchromosomen von Chironomus tentans durch Ecdyson. Exp. Cell Res. 20: 623–626.CrossRefGoogle Scholar
Doyle, D. and Laufer, H.. 1969. Requirements of ribonucleic acid synthesis for the formation of salivary gland specific proteins in the larvae Chironomus tentans. Exptl. Cell Res. 57: 205–210.CrossRefGoogle Scholar
Laufer, H. 1963. Hormones and the development of insects. Proc. 16th int. Congr. Zool. (Washington, D.C.), Vol. 4, pp. 215–220.Google Scholar
Laufer, H. 1965. Developmental studies of the dipteran salivary gland III. Relationships between chromosomal puffing and cellular function during development. In Developmental and metabolic control mechanisms and neoplasia. 19th M. D. Anderson Hosp. Symp. on Fundamental Cancer Res., Univ. of Texas, pp. 237–250.Google Scholar
Laufer, H. and Holt, T. K. H.. 1970. Juvenile hormone effects on chromosomal puffing and development in Chironomus thummi. J. exptl Zool. 173: 341–352.CrossRefGoogle ScholarPubMed
Laufer, H. and Nakase, Y.. 1965 a. Salivary gland secretion and its relation to chromosomal puffing in the dipteran Chironomus thummi. Proc. natn. Acad. Sci. 53: 511–516.CrossRefGoogle ScholarPubMed
Laufer, H. and Nakase, Y.. 1965 b. Developmental studies of the dipteran salivary gland II. DNase activity in Chironomus thummi. J. Cell. Biol. 25: 97–102.CrossRefGoogle ScholarPubMed
*Laufer, H., Nakase, Y., and Vanderberg, J.. 1964. Developmental studies of the dipteran salivary gland. 1: The effects of actinomycin D on larval development, enzyme activity, and chromosomal differentiation in Chironomus thummi. Devel. Biol. 9: 367–384.CrossRefGoogle Scholar
Laufer, H., Rao, B., and Nakase, Y.. 1967. Developmental studies of the dipteran salivary gland IV. Changes in DNA content. J. exp Zool. 166: 71–76.CrossRefGoogle ScholarPubMed
Laufer, H. and Wilson, M.. 1970. Hormonal control of gene activity as revealed by puffing of salivary gland chromosomes in dipteran larvae. In Laboratory experiments in general and comparative endocrinology. Peter, R. E. and Gorbman, A., Eds. pp. 185–200. Prentice-Hall, Englewood Cliffs, N.J.Google Scholar
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J.. 1951. Protein measurement with the Folin phenol reagent. J. biol. Chem. 193: 265–275.CrossRefGoogle ScholarPubMed
Rodems, A. E., Hendrikson, P. A., and Clever, U.. 1969. Proteolytic enzymes in the salivary gland of Chironomus tentans. Experientia 25: 686–687.CrossRefGoogle ScholarPubMed
Schin, K. S. and Clever, U.. 1965. Lysosomal and free acid phosphatase in salivary glands of Chironomus tentans. Science 150: 1053–1055.CrossRefGoogle ScholarPubMed
Schin, K. S. and Clever, U.. 1968. Ultrastructural and cytochemical studies of salivary gland regression in Chironomus tentans. Z. Zellforsch. 86: 262–279.CrossRefGoogle ScholarPubMed