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PATHOGENICITY OF THE IRELAND STRAIN OF NUCLEAR POLYHEDROSIS VIRUS TO SPRUCE BUDWORM, CHORISTONEURA FUMIFERANA, LARVAE

Published online by Cambridge University Press:  31 May 2012

P.M. Ebling ,
J.W. Barrett  and
B.M. Arif
P.M. Ebling
Affiliation:
Canadian Forest Service, Natural Resources Canada, P.O. Box 490, Sault Ste. Marie, Ontario, Canada P6A 5M7
J.W. Barrett
Affiliation:
Canadian Forest Service, Natural Resources Canada, P.O. Box 490, Sault Ste. Marie, Ontario, Canada P6A 5M7
B.M. Arif
Affiliation:
Canadian Forest Service, Natural Resources Canada, P.O. Box 490, Sault Ste. Marie, Ontario, Canada P6A 5M7
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Extract

The spruce budworm, Choristoneura fumiferana (Clem.), nuclear polyhedrosis virus (CfMNPV) has been studied extensively for its potential use as a bio-insecticide (Cunningham 1995). Recent advances in recombinant DNA technology have been impetuous in the genetic engineering of this virus to increase its virulence and (or) speed of action. This aspect of research has been concentrated on the modification of a plaque purified (Ireland strain) isolate obtained from the wild-type virus population (Arif et al. 1984). To assess any improvement in the effectiveness against the target pest, the virulence of the unaltered virus must first be determined. Bioassay results that have been previously reported by Kaupp and Ebling (1990) were conducted on the wild-type virus found in nature which consists of a mixture of viruses (Arif et al. 1994). Results of bioassays conducted to determine dose–response and time–response of fifth-instar spruce budworm larvae to the Ireland strain of CfMNPV are reported here.

Type
Articles
Information
The Canadian Entomologist , Volume 130 , Issue 1 , February 1998 , pp. 107 - 108
Copyright
Copyright © Entomological Society of Canada 1998

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