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ENZYME POLYMORPHISM IN THE CORN EARWORM, HELIOTHIS ZEA (LEPIDOPTERA: NOCTUIDAE). HEMOLYMPH ESTERASE POLYMORPHISM

Published online by Cambridge University Press:  31 May 2012

Douglas K. Sell ,
Gregory S. Whitt ,
Robert L. Metcalf  and
Lu-ping Kan Lee
Douglas K. Sell
Affiliation:
Illinois Natural History Survey and Illinois Agricultural Experiment Station, Urbana
Gregory S. Whitt
Affiliation:
Illinois Natural History Survey and Illinois Agricultural Experiment Station, Urbana
Robert L. Metcalf
Affiliation:
Illinois Natural History Survey and Illinois Agricultural Experiment Station, Urbana
Lu-ping Kan Lee
Affiliation:
Illinois Natural History Survey and Illinois Agricultural Experiment Station, Urbana
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Abstract

Esterases present in the hemolymph of last instar Heliothis zea (Boddie) were separated by polyacrylamide gel electrophoresis. Four systems, all polymorphic, were observed. One system, Est-II, appeared especially suited for the study of some of the important population processes of this species; these include host–plant relations, migration, and the effects of environmental pressures such as pesticides on the species.

Est-II was classified as a carboxylesterase, being sensitive only to organophosphate inhibitors. Dichlorvos at 10−5M completely inhibited Est-II activity.

Populations collected from corn from Tifton, Ga., and in two locations in Illinois, at approximately the same time were very similar genetically when compared on the basis of Est-II allele frequencies. A population collected from leguminous hosts in Tifton, Ga., at the same time as the above three populations was different genetically.

Est-IIc, an allele normally rare or absent in the populations studied, was present at a high frequency in one Urbana population collection. Est-IIc may be useful in studying the long-range dispersal of H. zea.

Type
Articles
Information
The Canadian Entomologist , Volume 106 , Issue 7 , July 1974 , pp. 701 - 709
Copyright
Copyright © Entomological Society of Canada 1974

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References

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