Hostname: page-component-76fb5796d-25wd4 Total loading time: 0 Render date: 2024-04-27T18:28:29.609Z Has data issue: false hasContentIssue false

AN EXTRACTION METHOD FOR RAPID COUNTS OF INSECT EGGS AND SMALL ORGANISMS

Published online by Cambridge University Press:  31 May 2012

S. F. Condrashoff
Affiliation:
Forest Research Laboratory, Department of Forestry and Development, Victoria, British Columbia

Abstract

Insect eggs and small organisms may be removed from foliage or bark with solvents and recovered on filter paper. Tests with Acleris variana (Fern.) showed that 96 per cent of eggs were recovered compared to 47–70 per cent counted on foliage. Tests indicated that the extraction method required one-third to one-tenth the time spent in counting the organisms on foliage. The extraction method was more consistent than counts on foliage, and less affected by differences in personnel and working conditions. The method and the solvent must be selected and modified to suit the organism.

Type
Articles
Copyright
Copyright © Entomological Society of Canada 1967

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Condrashoff, S. F., and Alexander, N. E.. 1966. Defoliation of western hemlock by a needle miner, Epinotia sp. Bi-mon. Prog. Rep. 22: 3.Google Scholar
Coveness, F. E., and Jensen, A. J.. 1955. Modification of the centrifugal-flotation technique for the isolation and concentration of nematodes and their eggs from soil and plant tissue. Proc. Helminthol. Soc. Wash. 22: 8789.Google Scholar
Kevan, D. K. McE. 1962. Soil animals. Philosophical Library, Inc., New York. XV, 237 pp.Google Scholar
Matteson, J. W. 1966. Flotation technique for extracting eggs of Diabrotica spp. and other organisms from soil. J. Econ. Ent. 59: 223224.CrossRefGoogle Scholar
Silver, G. T. 1959. A method for sampling eggs of the black-headed budworm. J. Forestry 57: 203205.Google Scholar