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Voltage-gated sodium channel gene mutation and P450 gene expression are associated with the resistance of Aphis spiraecola Patch (Hemiptera: Aphididae) to lambda-cyhalothrin

Published online by Cambridge University Press:  05 January 2024

Hong-cheng Tang ,
Yu-rong Zhou ,
Jun-feng Zuo ,
Yi-xuan Wang ,
Jaime C. Piñero ,
Xiong Peng  and
Mao-hua Chen Open the ORCID record for Mao-hua Chen [Opens in a new window]
Hong-cheng Tang
Affiliation:
Department of Entomology, National Key Laboratory of Crop Improvement for Stress Tolerance and Production, Key Laboratory of Crop Pest Integrated Pest Management on the Loess Plateau of Ministry of Agriculture and Rural Affairs, Key Lab Plant Protect Resources and Pest Management of Ministry of Education, College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi, China
Yu-rong Zhou
Affiliation:
Department of Entomology, National Key Laboratory of Crop Improvement for Stress Tolerance and Production, Key Laboratory of Crop Pest Integrated Pest Management on the Loess Plateau of Ministry of Agriculture and Rural Affairs, Key Lab Plant Protect Resources and Pest Management of Ministry of Education, College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi, China
Jun-feng Zuo
Affiliation:
Department of Entomology, National Key Laboratory of Crop Improvement for Stress Tolerance and Production, Key Laboratory of Crop Pest Integrated Pest Management on the Loess Plateau of Ministry of Agriculture and Rural Affairs, Key Lab Plant Protect Resources and Pest Management of Ministry of Education, College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi, China
Yi-xuan Wang
Affiliation:
Department of Entomology, National Key Laboratory of Crop Improvement for Stress Tolerance and Production, Key Laboratory of Crop Pest Integrated Pest Management on the Loess Plateau of Ministry of Agriculture and Rural Affairs, Key Lab Plant Protect Resources and Pest Management of Ministry of Education, College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi, China
Jaime C. Piñero
Affiliation:
Stockbridge School of Agriculture, University of Massachusetts, Amherst, MA 01003 USA
Xiong Peng*
Affiliation:
Department of Entomology, National Key Laboratory of Crop Improvement for Stress Tolerance and Production, Key Laboratory of Crop Pest Integrated Pest Management on the Loess Plateau of Ministry of Agriculture and Rural Affairs, Key Lab Plant Protect Resources and Pest Management of Ministry of Education, College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi, China
Mao-hua Chen*
Affiliation:
Department of Entomology, National Key Laboratory of Crop Improvement for Stress Tolerance and Production, Key Laboratory of Crop Pest Integrated Pest Management on the Loess Plateau of Ministry of Agriculture and Rural Affairs, Key Lab Plant Protect Resources and Pest Management of Ministry of Education, College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi, China
*
Corresponding authors: Mao-hua Chen; Email: maohua.chen@nwsuaf.edu.cn; Xiong Peng; Email: pengxiong@nwsaf.edu.cn
Corresponding authors: Mao-hua Chen; Email: maohua.chen@nwsuaf.edu.cn; Xiong Peng; Email: pengxiong@nwsaf.edu.cn
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Abstract

Aphis spiraecola Patch is one of the most economically important tree fruit pests worldwide. The pyrethroid insecticide lambda-cyhalothrin is commonly used to control A. spiraecola. In this 2-year study, we quantified the resistance level of A. spiraecola to lambda-cyhalothrin in different regions of the Shaanxi province, China. The results showed that A. spiraecola had reached extremely high resistance levels with a 174-fold resistance ratio (RR) found in the Xunyi region. In addition, we compared the enzymatic activity and expression level of P450 genes among eight A. spiraecola populations. The P450 activity of A. spiraecola was significantly increased in five regions (Xunyi, Liquan, Fengxiang, Luochuan, and Xinping) compared to susceptible strain (SS). The expression levels of CYP6CY7, CYP6CY14, CYP6CY22, P4504C1-like, P4506a13, CYP4CZ1, CYP380C47, and CYP4CJ2 genes were significantly increased under lambda-cyhalothrin treatment and in the resistant field populations. A L1014F mutation in the sodium channel gene was found and the mutation rate was positively correlated with the LC50 of lambda-cyhalothrin. In conclusion, the levels of lambda-cyhalothrin resistance of A. spiraecola field populations were associated with P450s and L1014F mutations. Our combined findings provide evidence on the resistance mechanism of A. spiraecola to lambda-cyhalothrin and give a theoretical basis for rational and effective control of this pest species.

Keywords

Aphis spiraecolalambda-cyhalothrinP450sodium channeltoxicity
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 114 , Issue 1 , February 2024 , pp. 49 - 56
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Copyright
Copyright © Northwest A&F University, 2024. Published by Cambridge University Press

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