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Three strains of Wolbachia pipientis and high rates of infection in Iranian sandfly species

Published online by Cambridge University Press:  03 February 2014

A. Bordbar ,
S. Soleimani ,
F. Fardid ,
M.R. Zolfaghari  and
P. Parvizi
A. Bordbar
Affiliation:
Molecular Systematics Laboratory, Parasitology Department, Pasteur Institute of Iran, Tehran, Iran
S. Soleimani
Affiliation:
Molecular Systematics Laboratory, Parasitology Department, Pasteur Institute of Iran, Tehran, Iran Microbiology Department, Qom Branch, Islamic Azad University, Qom, Iran
F. Fardid
Affiliation:
Molecular Systematics Laboratory, Parasitology Department, Pasteur Institute of Iran, Tehran, Iran
M.R. Zolfaghari
Affiliation:
Microbiology Department, Qom Branch, Islamic Azad University, Qom, Iran
P. Parvizi*
Affiliation:
Molecular Systematics Laboratory, Parasitology Department, Pasteur Institute of Iran, Tehran, Iran
*
*Author for Correspondence Phone: +00 98216649 6414 Fax: +00982166968855 E-mail: Parp@Pasteur.ac.ir
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Abstract

Individual wild-caught sandflies from Iran were examined for infections of Wolbachia pipientis by targeting the major surface protein gene wsp of this intracellular α-proteobacterium. In total, 638 male and female sandflies were screened, of which 241 were found to be positive for one of three wsp haplotypes. Regardless of geographical origins and habitats, Phlebotomus (Phlebotomus) papatasi and other sandfly species were found to be infected with one common, widespread strain of A-group W. pipientis (Turk 54, GenBank accession EU780683; AY288297). In addition, a new A-group haplotype (Turk07, GenBank accession KC576916) was isolated from Phlebotomus (Paraphlebotomus) mongolensis and Phlebotomus (Pa.) caucasicus, and a new B-group haplotype (AZ2331, GenBank accession JX488735) was isolated from Phlebotomus (Larroussius) perfiliewi. Therefore, Wolbachia was found to occur in at least three of the incriminated vectors of zoonotic cutaneous leishmaniasis and zoonotic visceral leishmaniasis in different geographical regions of Iran. It may provide a new tool for the future control of leishmaniasis.

Keywords

Wolbachia pipientisIranian sandflieswsp gene
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 104 , Issue 2 , April 2014 , pp. 195 - 202
Copyright
Copyright © Cambridge University Press 2014 

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