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A PCR-based approach to distinguish important Diadegma species (Hymenoptera: Ichneumonidae) associated with diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae)

Published online by Cambridge University Press:  09 March 2007

B. Wagener ,
A. Reineke ,
B. Löhr  and
C.P.W. Zebitz
B. Wagener*
Affiliation:
International Centre of Insect Physiology and Ecology, PO Box 30772, 00100 GPO Nairobi, Kenya University of Hohenheim, Institute of Phytomedicine, Department of Entomology, D-70593 Stuttgart, Germany
A. Reineke
Affiliation:
University of Hohenheim, Institute of Phytomedicine, Department of Entomology, D-70593 Stuttgart, Germany
B. Löhr
Affiliation:
International Centre of Insect Physiology and Ecology, PO Box 30772, 00100 GPO Nairobi, Kenya
C.P.W. Zebitz
Affiliation:
University of Hohenheim, Institute of Phytomedicine, Department of Entomology, D-70593 Stuttgart, Germany
*
*Fax: +254 20 860110 Email: bwagener@icipe.org
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Abstract

The diamondback moth, Plutella xylostella (Linnaeus) has a cosmopolitan distribution and is one of the major pests on cruciferous plants. Biological control, especially with species of the genus Diadegma, has been successfully employed in several parts of the world, mainly in South East Asia. The taxonomy of this genus based on classical morphological characters is still unclear and misidentifications are reported. In the present study seven Diadegma species associated with P. xylostella were separated using polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) analyses. The second internal transcribed spacer (ITS2) of the ribosomal DNA (rDNA) was successfully amplified in all 167 individuals and digested using 11 different restriction enzymes. One restriction enzyme (CfoI) showed different restriction profiles in all species and also between two population samples of D. mollipla (Holmgren) from eastern and southern Africa. In addition, a new Diadegma species associated with P. xylostella from Ethiopia was discovered.

Type
Review Article
Information
Bulletin of Entomological Research , Volume 94 , Issue 5 , October 2004 , pp. 465 - 471
Copyright
Copyright © Cambridge University Press 2004

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