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Molecular characterization of clones of the Myzus persicae complex (Hemiptera: Aphididae) differing in their ability to transmit the potato leafroll luteovirus (PLRV)

Published online by Cambridge University Press:  09 March 2007

L. Terradot ,
J.-C. Simon ,
N. Leterme ,
D. Bourdin ,
A.C.C. Wilson ,
J.-P. Gauthier  and
Y. Robert
L. Terradot
Affiliation:
Station de Pathologie végétale, Domaine de la Motte au Vicomte, 35653 Le Rheu Cédex, France
J.-C. Simon*
Affiliation:
Laboratoire de Zoologie, Institut National de la Recherche Agronomique, Domaine de la Motte au Vicomte, 35653 Le Rheu Cédex, France
N. Leterme
Affiliation:
Laboratoire de Zoologie, Institut National de la Recherche Agronomique, Domaine de la Motte au Vicomte, 35653 Le Rheu Cédex, France
D. Bourdin
Affiliation:
Station de Pathologie végétale, Domaine de la Motte au Vicomte, 35653 Le Rheu Cédex, France
A.C.C. Wilson
Affiliation:
Department of Biological Sciences, Macquarie University NSW 2109, Australia
J.-P. Gauthier
Affiliation:
Laboratoire de Zoologie, Institut National de la Recherche Agronomique, Domaine de la Motte au Vicomte, 35653 Le Rheu Cédex, France
Y. Robert
Affiliation:
Laboratoire de Zoologie, Institut National de la Recherche Agronomique, Domaine de la Motte au Vicomte, 35653 Le Rheu Cédex, France
*
* Fax: + 33 (0) 2 99 28 51 50 E-mail: jcsimon@zennes.inra.fr
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Abstract

A prerequisite to studying the specific interactions involved in the persistent transmission of luteoviruses such as the potato leafroll virus (PLRV) is the characterization of both the virus and its vectors. A range of techniques was used to assess genetic differentiation among 27 clones belonging to the Myzus persicae complex (M. persicae (Sulzer), M. antirrhinii (Macchiati) and M. nicotianaeBlackman) and showing different efficiencies in transmitting PLRV isolates. All M. persicae/M. nicotianae clones belonged to one of two karyotypes, both 2n = 12, either normal or carrying an autosomal translocation (A1,3), and all M. antirrhinii clones had 13 or 14 chromosomes. Amplified esterase 4 genes were detected by PCR–REN assay in M. persicae/M. nicotianae taxa, with gene expression being modified by methylation. Similarly, amplified E4 genes were revealed in M. antirrhinii but they all showed unmethylated. Two allozyme and 11 microsatellite loci discriminated 10 different genotypic classes among the 27 clones. Analysis of genetic relatedness between these genotypic classes revealed that M. nicotianae clones were very closely related to M. persicaeclones, whereas the genetic differentiation between M. antirrhinii and M. persicae was greater. The implications of these results for the taxonomic status of these genotypes within the complex, and the transmission of PLRV, are discussed.

Type
Research Article
Information
Bulletin of Entomological Research , Volume 89 , Issue 4 , April 1999 , pp. 355 - 363
Copyright
Copyright © Cambridge University Press 1999

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