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A method for the detection and measurement of insecticide resistance in larvae of Lucilia cuprina (Wied.) (Dipt., Calliphoridae)

Published online by Cambridge University Press:  10 July 2009

N. A. Roxburgh
Affiliation:
Biological and Chemical Research Institute, Department of Agriculture, Private Mail Bag 10, Rydalmere, N.S.W., Australia
G. J. Shanahan
Affiliation:
Biological and Chemical Research Institute, Department of Agriculture, Private Mail Bag 10, Rydalmere, N.S.W., Australia

Abstract

In this method the toxicant is absorbed with blood serum onto a loose roll of chromatography paper; first-instar larvae of Lucilia cuprina (Wied.) are inserted into the air spaces of the roll and allowed to feed. Mortality of the larvae is assessed 24 h later. Typical results using diazinon, dichlofenthian and chlorfenvinphos against susceptible and resistant strains of L. cuprina showed larvae to have resistance factors 6–8 times higher than those for adults determined by established techniques.

Type
Research Paper
Copyright
Copyright © Cambridge University Press 1973

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