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Inheritance and activity of some esterases associated with organophosphate resistance in mosquitoes of the complex of Culex pipiens L. (Diptera: Cnlicidae)

Published online by Cambridge University Press:  10 July 2009

F. Villani ,
G. B. White ,
C. F. Curtis  and
S. J. Miles
F. Villani
Affiliation:
London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK.
G. B. White
Affiliation:
London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK.
C. F. Curtis
Affiliation:
London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK.
S. J. Miles
Affiliation:
London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK.
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Abstract

Eighteen strains of the complex of Culex pipiens L. from Africa, Asia and Europe were bioassayed for resistance to chlorpyrifos and electro-phoresed and stained for esterases. Susceptible strains showed only low activity esterase bands. The resistant strains of C. quinquefasciatus Say from hot countries (Liberia, Nigeria, Sri Lanka, Tanzania, Thailand) all showed the same two high intensity esterase bands (Rm 0·60 + 0·82). Different patterns of high esterase were found in resistant C. pipiens strains from cooler localities in Nairobi, Kenya (Rm 100), and Mont-pellier, France (Rm 0–50). Selection experiments on strains originally polymorphic for resistance and esterase pattern showed, without exception, that high esterase remained associated with resistance, and it is concluded that the association is almost certainly causal and not merely due to genetic linkage. The high intensity esterase bands were probably due to alleles of the loci Est-l, Est-2 and Est-3, separated by crossover distances of approximately 2·4 and 5·5 units, respectively. Strains monomorphic for what appeared to be the same high esterase pattern varied markedly in resistance level. Enzyme assays showed a direct relationship between levels of enzyme activity and resistance. Bioassays with fenthion and chlorpyrifos revealed differences in the relative resistance of C. quinquefasciatus from Colombo (Sri Lanka) and Dar-es-Salaam (Tanzania). Despite these differential degrees of cross-effectiveness, it is concluded that high intensity esterases are reliable indicators of organophosphate resistance in mosquitoes of the C. pipiens complex, although the possibility of other resistance mechanisms means that the lack of abnormally active esterases does not necessarily indicate the absence of resistance.

Type
Original Articles
Information
Bulletin of Entomological Research , Volume 73 , Issue 1 , March 1983 , pp. 153 - 170
Copyright
Copyright © Cambridge University Press 1983

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