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Inferring the population structure of Myzus persicae in diverse agroecosystems using microsatellite markers

Published online by Cambridge University Press:  01 March 2013

Juan Antonio Sanchez ,
Michelangelo La-Spina ,
Pedro Guirao  and
Fernando Cánovas
Juan Antonio Sanchez*
Affiliation:
Departamento de Biotecnología y Protección de Cultivos, Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA), C/Mayor, 1, 30150 La Alberca (Murcia), Spain
Michelangelo La-Spina
Affiliation:
Departamento de Biotecnología y Protección de Cultivos, Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA), C/Mayor, 1, 30150 La Alberca (Murcia), Spain
Pedro Guirao
Affiliation:
Departamento de Producción Vegetal y Microbiología, Escuela Politécnica Superior de Orihuela, Universidad Miguel Hernández, Ctra. de Beniel, km 3,2, 03312 Orihuela, Alicante
Fernando Cánovas
Affiliation:
Departamento de Biotecnología y Protección de Cultivos, Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA), C/Mayor, 1, 30150 La Alberca (Murcia), Spain CCMAR, CIMAR-Laboratório Associado, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal
*
*Author for correspondence: Juan Antonio Sanchez Phone: +34 968 362787 Fax: +34 968 366792 E-mail: cagitan2@hotmail.com; juana.sanchez23@carm.es
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Abstract

Diverse agroecosystems offer phytophagous insects a wide choice of host plants. Myzus persicae is a polyphagous aphid common in moderate climates. During its life cycle it alternates between primary and secondary hosts. A spatial genetic population structure may arise due to environmental factors and reproduction modes. The aim of this work was to determine the spatial and temporal genetic population structure of M. persicae in relation to host plants and climatic conditions. For this, 923 individuals of M. persicae collected from six plant families between 2005 and 2008 in south-eastern Spain were genotyped for eight microsatellite loci. The population structure was inferred by neighbour-joining, analysis of molecular variance (AMOVA) and Bayesian analyses. Moderate polymorphism was observed for the eight loci in almost all the samples. No differences in the number of alleles were observed between primary and secondary hosts or between geographical areas. The proportion of unique genotypes found in the primary host was similar in the north (0.961 ± 0.036) and the south (0.987 ± 0.013), while in the secondary host it was higher in the north (0.801 ± 0.159) than in the south (0.318 ± 0.063). Heterozygosity excess and linkage disequilibrium suggest a high representation of obligate parthenogens in areas with warmer climate and in the secondary hosts. The FST-values pointed to no genetic differentiation of M. persicae on the different plant families. FST-values, AMOVA and Bayesian model-based cluster analyses pointed to a significant population structure that was related to primary and secondary hosts. Differences between primary and secondary hosts could be due to the overrepresentation of parthenogens on herbaceous plants.

Keywords

hemipteraaphidshost plantsparthenogenesismolecular diversityclimate
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 103 , Issue 4 , August 2013 , pp. 473 - 484
Copyright
Copyright © Cambridge University Press 2013 

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