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Hybridization between Helicoverpa armigera and Helicoverpa assulta (Lepidoptera: Noctuidae): development and morphological characterization of F1 hybrids

Published online by Cambridge University Press:  09 March 2007

X.-C. Zhao ,
J.-F. Dong ,
Q.-B. Tang ,
Y.-H. Yan ,
I. Gelbic ,
J.J.A. Van Loon  and
C.-Z. Wang
X.-C. Zhao
Affiliation:
State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China Graduate School of the Chinese Academy of Sciences, Beijing 100039, China
J.-F. Dong
Affiliation:
State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China
Q.-B. Tang
Affiliation:
State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China Graduate School of the Chinese Academy of Sciences, Beijing 100039, China
Y.-H. Yan
Affiliation:
State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China
I. Gelbic
Affiliation:
Department of Morphology, Institute of Entomology AS CR, 370 05 Ceske Budejovice, Czech Republic
J.J.A. Van Loon
Affiliation:
Laboratory of Entomology, Wageningen University, Wageningen, The Netherlands
C.-Z. Wang*
Affiliation:
State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China
*
*Fax: + 86 10 6256 5689 E-mail: czwang@ioz.ac.cn
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Abstract

Reciprocal hybridizations between Helicoverpa armigera (Hübner) and Helicoverpa assulta (Guenée) were studied. The cross between females of H. armigera and males of H. assulta yielded only fertile males and sterile individuals lacking an aedeagus, valva or ostium bursae. A total of 492 larvae of the F1 generation were obtained and 374 of these completed larval development and pupated. Only 203 pupae were morphologically normal males, the remaining 171 pupae were malformed. Larvae and pupae that gave rise to morphologically abnormal adults exhibited longer development times. Sterility was not only associated with malformed external sex organs, but also a range of abnormalities of the internal reproductive system: (i) loss of internal reproductive organs, (ii) with one to three copies of an undeveloped bursa copulatrix; or (iii) with one or two undeveloped testes. Normal male hybrid adults showed higher flight activity in comparison with males of both species. In contrast, the cross between females of H. assulta and males of H. armigera yielded morphologically normal offspring (80 males and 83 females). The interaction of the Z-chromosome from H. assulta with autosomes from H. armigera might result in morphological abnormalities found in hybrids and backcrosses, and maternal-zygotic incompatibilities might contribute to sex bias attributed to hybrid inviability.

Keywords

Helicoverpa armigeraHelicoverpa assultaNoctuidaeLepidopterainterspecific hybridmorphologysterility
Type
Research Article
Information
Bulletin of Entomological Research , Volume 95 , Issue 5 , October 2005 , pp. 409 - 416
Copyright
Copyright © Cambridge University Press 2005

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