Hostname: page-component-76fb5796d-2lccl Total loading time: 0 Render date: 2024-04-26T07:01:42.947Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of acaricidal components isolated from lettuce (Lactuca sativa) on carmine spider mite (Tetranychus cinnabarinus Boisd.)

Published online by Cambridge University Press:  14 August 2017

M. Li ,
Y. Zhang ,
W. Ding ,
J. Luo ,
S. Li  and
Q. Zhang
M. Li
Affiliation:
College of Plant Protection, Southwest University, Chongqing 400716, People's Republic of China
Y. Zhang
Affiliation:
College of Plant Protection, Southwest University, Chongqing 400716, People's Republic of China Institute of Pesticide, Southwest University, Chongqing 400716, People's Republic of China
W. Ding*
Affiliation:
College of Plant Protection, Southwest University, Chongqing 400716, People's Republic of China Institute of Pesticide, Southwest University, Chongqing 400716, People's Republic of China
J. Luo
Affiliation:
College of Plant Protection, Southwest University, Chongqing 400716, People's Republic of China Institute of Pesticide, Southwest University, Chongqing 400716, People's Republic of China
S. Li
Affiliation:
College of Plant Protection, Southwest University, Chongqing 400716, People's Republic of China
Q. Zhang
Affiliation:
College of Plant Protection, Southwest University, Chongqing 400716, People's Republic of China
*
*Author for correspondence Phone: +86-023-68250953 Fax: +86-023-68250218 E-mail: weiding2013@hotmail.com
Get access Rights & Permissions [Opens in a new window]

Abstract

This study aimed to evaluate the acaricidal activity of lettuce (Lactuca sativa) extracts against carmine spider mites (Tetranychus cinnabarinus Boisd.) and isolate the acaricidal components. Acaricidal activities of lettuce extracts isolated from different parts (the leaf, root and seed) using various solvents (petroleum ether, acetone and methanol) were evaluated with slide-dip bioassay and relatively high median lethal concentration (LC50) values were detected. Acetone extracts of lettuce leaves harvested in July and September were fractionated and isolated with silica gel and thin-layer chromatography. Consequently, acetone extracts of lettuce leaves harvested in July exhibited higher acaricidal activity than those harvested in September, with an LC50 value of 0.268 mg ml−1 at 72 h post-treatment. A total of 27 fractions were obtained from the acetone extract of lettuce leaves harvested in July, and mite mortalities with the 11th and 12th fractions were higher than those with the other 25 fractions (LC50: 0.751 and 1.258 mg ml−1 at 48 h post-treatment, respectively). Subsequently, active acaricidal components of the 11th fraction were identified by infrared, nuclear magnetic resonance and liquid chromatography/mass spectrometry. Five components were isolated from the 11th fraction, with components 11-a and 11-b showing relatively high acaricidal activities (LC50: 0.288 and 0.114 mg ml−1 at 48 h post-treatment, respectively). Component 11-a was identified as β-sitosterol. In conclusion, acetone extracts of lettuce leaves harvested in July might be used as a novel phytogenic acaricide to control mites.

Keywords

Lactuca sativaTetranychus cinnabarinusacetoneacaricidal activityβ-sitosterol
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 108 , Issue 3 , June 2018 , pp. 314 - 320
Check for updates
Copyright
Copyright © Cambridge University Press 2017 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

†

These authors contributed equally to this work.

References

Abbott, W.S. (1987) A method of computing the effectiveness of an insecticide. 1925. Journal of the American Mosquito Control Association 3, 302–303.Google ScholarPubMed
Abdul, R.A., Gopalakrishnan, G., Venkatesan, P. & Geetha, K. (2008) Isolation and identification of mosquito larvicidal compound from Abutilon indicum (Linn.) Sweet. Parasitology Research 102, 981–988.CrossRefGoogle Scholar
Afify, A.E.M.M., El-Beltagi, H.S., Fayed, S.A. & Shalaby, E.A. (2011) Acaricidal activity of different extracts from Syzygium cumini L. Skeels (Pomposia) against Tetranychus urticae Koch. Asian Pacific Journal of Tropical Biomedicine 1, 359–364.CrossRefGoogle ScholarPubMed
Annonymous (1980) Plant Production and Protection 21, Recommended Methods for Measurement of Resistance to Pesticide. pp. 49–54. Israel, FAO.Google Scholar
Benelli, G., Pavela, R., Canale, A. & Mehlhorn, H. (2016) Tick repellents and acaricides of botanical origin: a green roadmap to control tick-borne diseases? Parasitology Research 115, 2545–2560.CrossRefGoogle Scholar
Bu, C., Li, J., Wang, X.Q., Shi, G., Peng, B., Han, J., Gao, P. & Wang, Y. (2015) Transcriptome analysis of the Carmine Spider Mite, Tetranychus cinnabarinus (Boisduval, 1867) (Acari: Tetranychidae), and its response to beta-Sitosterol. Biomed Research International 2015, 794718.CrossRefGoogle ScholarPubMed
Cavalcanti, S.C.H., Niculau, E.D.S., Blank, A.F., Câmara, C.A.G., Araújo, I.N. & Alves, P.B. (2010) Composition and acaricidal activity of Lippia sidoides essential oil against two-spotted spider mite (Tetranychus urticae Koch). Bioresource Technology 101, 829–832.CrossRefGoogle ScholarPubMed
Chen, Z.Z., Deng, Y.X., Yin, Z.Q., Wei, Q., Li, M., Jia, R.Y., Xu, J., Li, L., Song, X. & Liang, X.X. (2014) Studies on the acaricidal mechanism of the active components from neem (Azadirachta indica) oil against Sarcoptes scabiei var. cuniculi. Veterinary Parasitology 204, 323.CrossRefGoogle ScholarPubMed
Dantas, A.C.S., Machado, D.M.R., Araujo, A.C., Oliveira-Junior, R.G., Lima-Saraiva, S.R.G., Ribeiro, L.A.A., Almeida, J.R.G.S. & Horta, M.C. (2015) Acaricidal activity of extracts from the leaves and aerial parts of Neoglaziovia variegata (Bromeliaceae) on the cattle tick Rhipicephalus (Boophilus) microplus. Research in Veterinary Science 100, 165–168.CrossRefGoogle ScholarPubMed
Diaz, J.H. (2016) Chemical and plant-based insect repellents: efficacy, safety, and toxicity. Wilderness and Environmental Medicine 27, 153–163.CrossRefGoogle ScholarPubMed
Ding, L.J., Ding, W., Zhang, Y.Q. & Luo, J.X. (2013) Bioguided fractionation and isolation of esculentoside P from Phytolacca americana L. Industrial Crops and Products 44, 534–541.CrossRefGoogle Scholar
Duan, D.D., Bu, C.Y., Ma, L.Q., Liu, Y.B., Wang, Y.N. & Shi, G.L. (2012) Identification of Acarcidal Compounds in Inula Japonica Extracts Against Tetranychus Cinnabarinus. Springer, Berlin, Heidelberg.CrossRefGoogle Scholar
Edziri, H., Smach, M., Ammar, S., Mahjoub, M., Mighri, Z., Aouni, M. & Mastouri, M. (2011) Antioxidant, antibacterial, and antiviral effects of Lactuca sativa extracts. Industrial Crops and Products 34, 1182–1185.CrossRefGoogle Scholar
Feng, G., Zhang, J., Bai, J. & Peng, Z.Q. (2009) Isolation and identification of acaricidal composition of thymus mongolicus. Acta Bot. Boreal.-Occident. Sin 29, 1893–1897.Google Scholar
Ferraz, A.D.B.F., Balbino, J.M., Zini, C.A., Ribeiro, V.L.S., Bordignon, S.A.L. & Poser, G.V. (2010) Acaricidal activity and chemical composition of the essential oil from three Piper species. Parasitology Research 107, 243–248.CrossRefGoogle Scholar
Hou, Q., Wang, D., Zhang, B., Wei, D. & Zhang, Y. (2015) Biochemical evidences for scopoletin lnhibits Ca2+-ATPase activity in the carmine spider mite, Tetranychus cinnabarinus (Boisduval). Agricultural Science and Technology 16, 826–831.Google Scholar
Huili, G., Guanglu, S., Liangxi, J., Dongfeng, W. & Younian, W. (2012) Evaluation acaricidal activities of Momordica cochinchinensis extracts against Tetranychus cinnabarinus. Journal of Ecology 32, 2883–2889.Google Scholar
Kinoshita, S., Koura, Y., Kariya, H., Ohsaki, N. & Watanabe, T. (1999) AKD-2023: a novel miticide. Biological activity and mode of action. Pesticide Science 55, 659–660.3.0.CO;2-S>CrossRefGoogle Scholar
Kwon, D.H., Clark, J.M. & Si, H.L. (2014) Toxicodynamic mechanisms and monitoring of acaricide resistance in the two-spotted spider mite. Pesticide Biochemistry and Physiology 121, 97–101.CrossRefGoogle ScholarPubMed
Lai, R. & You, M. (2004) Anti-feeding effect of the extracts from non-preferable plants on adults of the striped flea beetle [{\ sl Phyllotreta striolata}(F.)]. Plant Protection 31, 37–40.Google Scholar
Lapornik, B., Prošek, M. & Golc Wondra, A. (2005) Comparison of extracts prepared from plant by-products using different solvents and extraction time. Journal of Food Engineering 71, 214–222.CrossRefGoogle Scholar
Liu, M.Y., Hu, G.F., Yu, H.T., Niu, S.J. & Li, Y.Q. (2012) A study on ovicidal activities of extracts from 35 species of plants (including Coronilla varia) against Mythimna separata. Acta Prataculturae Sinica 21, 198–205.Google Scholar
Liu, X., Ardo, S., Bunning, M., Parry, J., Zhou, K., Stushnoff, C., Stoniker, F., Yu, L. & Kendall, P. (2007 a) Total phenolic content and DPPH radical scavenging activity of lettuce (Lactuca sativa L.) grown in Colorado. LWT – Food Science and Technology 40, 552–557.CrossRefGoogle Scholar
Liu, X., Ardo, S., Bunning, M., Parry, J., Zhou, K., Stushnoff, C., Stoniker, F., Yu, L. & Kendall, P. (2007 b) Total phenolic content and DPPH radical scavenging activity of lettuce (Lactuca sativa L.) grown in Colorado. LWT-Food Science and Technology 40, 552–557.CrossRefGoogle Scholar
Llorach, R., Gil, M.I. & Ferreres, F. (2008) Characterisation of polyphenols and antioxidant properties of five lettuce varieties and escarole. Food Chemistry 108, 1028–1038.CrossRefGoogle ScholarPubMed
Mathivanan, D., Gandhi, P.R., Mary, R.R. & Suseem, S.R. (2017) Larvicidal and acaricidal efficacy of different solvent extracts of Andrographis echioides against blood-sucking parasites. Physiological and Molecular Plant Pathology. Available online.Google Scholar
Nicolle, C., Cardinault, N., Gueux, E., Jaffrelo, L., Rock, E., Mazur, A., Amouroux, P. & Rémésy, C. (2004) Health effect of vegetable-based diet: lettuce consumption improves cholesterol metabolism and antioxidant status in the rat. Clinical Nutrition 23, 605–614.CrossRefGoogle ScholarPubMed
Nicolopouloustamati, P., Maipas, S., Kotampasi, C., Stamatis, P. & Hens, L. (2016) Chemical pesticides and human health: the urgent need for a new concept in agriculture. Frontiers in Public Health 4, 148.Google ScholarPubMed
Oyinlola, L.A., Obadina, A.O., Omemu, A.M. & Oyewole, O.B. (2017) Prevention of microbial hazard on fresh-cut lettuce through adoption of food safety and hygienic practices by lettuce farmers. Food Science Nutrition 5, 67–75.CrossRefGoogle ScholarPubMed
Ren, J.J., Shi, G.L., Wang, J.W., Gu, J.C. & Wang, Y.N. (2009 a) Isolation and identification of the principal acaricidal components extracted from Mentha piperita. Scientia Silvae Sinicae 92, 77–82.Google Scholar
Ren, J.J., Shi, G.L., Wang, J.W., Gu, J.C. & Wang, Y.N. (2009 b) Isolation and identification of the principal acaricidal components extracted from Mentha piperita. Scientia silvae Sinicae 45, 77–82.Google Scholar
SAS, I. (2000) SAS OnlineDoc®, Version 8.01. Cary, North Carolina, USA, Statistical Analysis System Institute.Google Scholar
Sayyah, M., Hadidi, N. & Kamalinejad, M. (2004) Analgesic and anti-inflammatory activity of Lactuca sativa seed extract in rats. Journal of Ethnopharmacology 92, 325–329.CrossRefGoogle ScholarPubMed
Schmidt, R.A. (2014) Leaf structures affect predatory mites (Acari: Phytoseiidae) and biological control: a review. Experimental and Applied Acarology 62, 1–17.CrossRefGoogle ScholarPubMed
Sen, A., Dhavan, P., Shukla, K.K., Singh, S. & Tejovathi, G. (2013) Analysis of IR, NMR and antimicrobial activity of β-sitosterol isolated from Momordica charantia. Sciencesecurejournals Com 1, 9–13.Google Scholar
Serafini, M., Bugianesi, R., Salucci, M., Azzini, E., Raguzzini, A. & Maiani, G. (2002) Effect of acute ingestion of fresh and stored lettuce (Lactuca sativa) on plasma total antioxidant capacity and antioxidant levels in human subjects. British Journal of Nutrition 88, 615–623.CrossRefGoogle ScholarPubMed
Singh, R.N. & Saratchandra, B. (2005) The development of botanical products with special reference to seri-ecosystem. Caspian Journal of Environmental Sciences 3, 1–8.Google Scholar
Stewart, J., Shipley, C., Ireland, F., Jarrell, V., Timlin, C., Shike, D. & Felix, T. (2016) Long-term effects of pyrethrin and cyfluthrin, a type II synthetic pyrethroid, insecticide applications on bull reproductive parameters. Reproduction in Domestic Animals = Zuchthygiene 51, 680–687.CrossRefGoogle ScholarPubMed
Thoison, O., Sevenet, T., Niemeyer, H.M. & Russell, G.B. (2004) Insect antifeedant compounds from Nothofagus dombeyi and N. pumilio. Phytochemistry 65, 2173–2176.CrossRefGoogle ScholarPubMed
Trivedi, P.C. & Choudhrey, N. (2011) Isolation and characterization of bioactive compound β-sitosterol from Lithuania Somnifera L. Journal of Pharmacy Research 4, 4252–4253.Google Scholar
Wang, D.D., Jia, F.L., Chen, J., Yu, W.J. & Dai, G.H. (2013) Acaricidal activities of Albizzia julibrissin Durazz extracts against Tetranychus cinnabarinus (Acari: Tetranychidae). Advanced Materials Research 666, 77–84.CrossRefGoogle Scholar
Wang, Y., Shi, G., Zhao, L., Liu, S., Yu, T., Clarke, S. & Sun, J. (2007 a) Acaricidal activity of Juglans regia leaf extracts on Tetranychus viennensis and Tetranychus cinnabarinus (Acari: Tetranychidae). Journal of Economic Entomology 100, 1298–1303.CrossRefGoogle Scholar
Wang, Y.N., Shi, G.L., Zhao, L.L., Liu, S.Q., Yu, T.Q., Clarke, S.R. & Sun, J.H. (2007 b) Acaricidal activity of Juglans regia leaf extracts on Tetranychus viennensis and Tetranychus cinnabarinus (Acari: Tetranychidae). Journal of Economic Entomology 100, 1298–1303.CrossRefGoogle Scholar
Yu, H., Yue, Y., Dong, X., Li, R. & Li, P. (2016) The Acaricidal Activity of Venom from the Jellyfish Nemopilema nomurai against the Carmine Spider Mite Tetranychus cinnabarinus. Toxins 8, 179.CrossRefGoogle ScholarPubMed
Zahir, A.A., Rahuman, A.A., Kamaraj, C., Bagavan, A., Elango, G., Sangaran, A. & Kumar, B.S. (2009) Laboratory determination of efficacy of indigenous plant extracts for parasites control. Parasitology Research 105, 453–461.CrossRefGoogle ScholarPubMed
Zhang, Y.-Q., Ding, W., Zhao, Z.-M., Wu, J. & Fan, Y.-H. (2008) Studies on Acaricidal Bioactivities of Artemisia annua L. extracts against Tetranychus cinnabarinus Bois (Acari: Tetranychidae). Agricultural Sciences in China 7, 577–584.CrossRefGoogle Scholar