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DNA barcodes for two scale insect families, mealybugs (Hemiptera: Pseudococcidae) and armored scales (Hemiptera: Diaspididae)

Published online by Cambridge University Press:  28 January 2011

D.-S. Park ,
S.-J. Suh ,
P.D.N. Hebert ,
H.-W. Oh  and
K.-J. Hong
D.-S. Park
Affiliation:
Biological Resource Center, KRIBB, Daejeon 305-806, Korea
S.-J. Suh
Affiliation:
Youngnam Regional Office, National Plant Quarantine Service, Busan 600-016, Korea
P.D.N. Hebert
Affiliation:
Biodiversity Institute of Ontario, University of Guelph, Guelph, Ontario, N1G 2W1, Canada
H.-W. Oh*
Affiliation:
Industrial Biomaterials Research Center, KRIBB, 111 Gwahangno, Yuseong-gu, Daejeon 305-806, Korea
K.-J. Hong*
Affiliation:
Central Post-entry Quarantine Station, National Plant Quarantine Service, 433-1 Anyang 6-dong Manan-gu Anyang-shi, Gyeonggi Province, 430-016, Korea
*
*Authors for correspondence Fax: +82-42-860-4677 (H.-W.O);+82-31-420-7606 (K.-J.H.) E-mail: hwoh@kribb.re.kr
curcul@hanmail.net
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Abstract

Although DNA barcode coverage has grown rapidly for many insect orders, there are some groups, such as scale insects, where sequence recovery has been difficult. However, using a recently developed primer set, we recovered barcode records from 373 specimens, providing coverage for 75 species from 31 genera in two families. Overall success was >90% for mealybugs and >80% for armored scale species. The G·C content was very low in most species, averaging just 16.3%. Sequence divergences (K2P) between congeneric species averaged 10.7%, while intra-specific divergences averaged 0.97%. However, the latter value was inflated by high intra-specific divergence in nine taxa, cases that may indicate species overlooked by current taxonomic treatments. Our study establishes the feasibility of developing a comprehensive barcode library for scale insects and indicates that its construction will both create an effective system for identifying scale insects and reveal taxonomic situations worthy of deeper analysis.

Keywords

DNA barcodingCOImealybugarmored scaleNUMTsG·C content
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 101 , Issue 4 , August 2011 , pp. 429 - 434
Copyright
Copyright © Cambridge University Press 2011

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