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Breeding structure of Glossina pallidipes (Diptera: Glossinidae) populations in East and southern Africa

Published online by Cambridge University Press:  10 July 2009

E. S. Krafsur ,
N. Griffiths ,
C. L. Brockhouse  and
J. Brady
E. S. Krafsur
Affiliation:
Department of Entomology, Iowa State University, Ames, Iowa 50011-3222, USA:
N. Griffiths
Affiliation:
Department of Biology, Imperial College of Science, Medicine and Technology, Silwood Park, Ascot, Berks, SL5 7PY, UK
C. L. Brockhouse
Affiliation:
Department of Entomology, Iowa State University, Ames, Iowa 50011-3222, USA:
J. Brady
Affiliation:
Department of Biology, Imperial College of Science, Medicine and Technology, Silwood Park, Ascot, Berks, SL5 7PY, UK
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Abstract

Gene diversity and gene flow in Glossina pallidipes Austen were studied by using allozyme electrophoresis on samples from southern and East Africa. Recorded were 30 alleles segregating at eight loci. Gene diversity was 0.212±0.085 S.E. in four southern African populations and 0.163±0.076 in seven Kenyan populations. All loci were in Hardy-Weinberg equilibrium. Pgm and 6pgd were sex linked. Spatial components of gene diversity were measured by using F statistics. Mating was random within each population. The ‘fixation index’ FST was 0.133±0.062 among southern African populations. Among the Kenyan populations. FST was 0.159±0.069. FST was 0.238±0.051 among all populations. Analysis of variance of gene frequencies showed that 65.8% of the genetic variance lay within populations and 34.2% of the genetic variance lay between Kenyan and southern African populations. These data suggest a strong measure of genetic drift among tsetse populations even in a region where it is thought they are continuously distributed. The causes of this drift require investigation.

Type
Original Articles
Information
Bulletin of Entomological Research , Volume 87 , Issue 1 , February 1997 , pp. 67 - 73
Copyright
Copyright © Cambridge University Press 1997

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References

Agatsuma, T. & Otieno, L.H. (1988) Isoenzyme studies on two field populations of Glossina pallidipes Austen (Diptera: Glossinidae) in Kenya. Insect Science Application 9, 527–530.Google Scholar
Black, W.C. IV & Krafsur, E.S. (1985a). Electrophoretic analysis of genetic variability in the house fly (Musca domestica L.). Biochemical Genetics 23, 193–203.Google Scholar
Black, W.C. IV & Krafsur, E.S. (1985b) A FORTRAN program for analysis of genotypic frequencies and description of the breeding structure of populations. Theoretical and Applied Genetics 70, 484–490.Google Scholar
Blanchetot, A. & Gooding, R.H. (1995) Identification of a mariner element from the tsetse fly, Glossina palpalis palpalis. Insect Molecular Biology 4, 89–96.CrossRefGoogle ScholarPubMed
Clayton, J.W. & Tretiak, D.N. (1972) Amine-citrate buffers for pH control in starch gel electrophoresis. Journal of the Fisheries Research Board, Canada 29, 1169–1172.CrossRefGoogle Scholar
Colvin, J. & Gibson, G. (1992) Host-seeking behavior and management of tsetse. Annual Review of Entomology 37, 21–40.Google Scholar
Crow, J.F. & Aoki, K. (1984) Group selection for a polygenic behavioral trait: estimating the degree of population subdivision. Proceedings of the National Academy of Sciences, USA 81, 6073–6077.Google Scholar
Ford, J. (1971) The role of the trypanosomes in African ecology. A study of the tsetse fly problem. 568 pp. Oxford, Clarendon Press.Google Scholar
Ford, J., Maudlin, I. & Humphryes, K.C. (1972) Comparisons between three small collections of Glossina morsitans morsitans (Machado) (Diptera: Glossinidae) from the Kilombero River Valley, Tanzania. Part 1. Characteristics of flies exhibiting different patterns of behaviour. Acta Tropica 29, 231–249.Google Scholar
Griffiths, N. & Brady, J. (1994) Analysis of the components of ‘electric nets’ that affect their sampling efficiency for tsetse flies (Diptera: Glossinidae). Bulletin of Entomological Research 84, 325–330.Google Scholar
Hartl, D. & Clark, A.G. (1989) Principles of population genetics, 2nd edn.Sunderland, Massachusetts, Sinauer Associates.Google Scholar
Jordan, A.M. (1993) Tsetse-flies (Glossinidae), pp. 333–388in Land, R.P. & Crosskey, R.W. (Eds) Medical insects and arachnids. London, Chapman & Hall.Google Scholar
Jordan, A.M., Trewern, M.A., Southern, D.I., Pell, P.E. & Davies, E.D.G. (1977) Differences in laboratory performance between strains of Glossina morsitans morsitans Westwood from Rhodesia and Tanzania and associated chromosome diversity. Bulletin of Entomological Research 57, 35–48.CrossRefGoogle Scholar
Langley, P.A., Maudlin, I. & Leedham, M.P. (1984) Genetic and behavioural differences between Glossina pallidipes from Uganda and Zimbabwe. Entomologia Experimentalis et Applicata 35, 55–60.CrossRefGoogle Scholar
Maudlin, I. (1970) Preliminary studies on the karyotypes of five species of Glossina. Parasitology 61, 71–74.Google Scholar
Maudlin, I. (1979) Chromosome polymorphism and sex determination in a wild population of tsetse. Nature 277, 300–301.Google Scholar
Munstermann, L.E. (1979) Isozymes of Aedes aegypti: phenotypes, linkage and use in the genetic analysis of sympatric subspecies populations in East Africa. 176 pp. PhD Dissertation, University of Notre Dame.Google Scholar
Murphy, R.W., Sites, J.W., Buth, D.G. & Haufler, C.H. (1990) Proteins I: Isozyme electrophoresis. pp. 45–126in Hillis, D.M. & Moritz, C. (Eds) Molecular systematics. Sunderland, Massachusetts, Sinauer Associates, Inc.Google Scholar
Nei, M. (1977) F-statistics and analysis of gene diversity in subdivided populations. Annals of Human Genetics 41, 225–233.CrossRefGoogle ScholarPubMed
Nei, M. (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89, 583–590.CrossRefGoogle ScholarPubMed
Nei, M. (1987) Molecular population genetics and evolution. New York, Columbia University Press.CrossRefGoogle Scholar
Nesbitt, S.A.T., Gooding, R.H. & Rolseth, B.M. (1990) Genetic variation in two field populations and a laboratory colony of Glossina pallidipes (Diptera: Glossinidae). Journal of Medical Entomology 27, 586–591.CrossRefGoogle Scholar
O'Neill, S., Gooding, R.H. & Aksoy, S. (1993) Phylogenetically distant symbiotic microorganisms reside in Glossina midgut and ovary tissue. Medical and Veterinary Entomology 7, 377–383.CrossRefGoogle Scholar
Slatkin, M. (1985) Gene flow in natural populations. Annual Review of Ecology and Systematics 16, 393–430.CrossRefGoogle Scholar
Slatkin, M. (1987) Gene flow and the geographic structure of natural populations. Science 236, 787–792.CrossRefGoogle ScholarPubMed
Southern, D.I. (1981) Chromosome diversity in tsetse flies, pp. 225–243in Blackman, R, Hewitt, G. & Ashburner, M. (Eds) Insect cytogenetics. Symposium of the Royal Entomological Society of London, Blackwell.Google Scholar
Southern, D.I. & Pell, E. (1981) Cytogenetical aspects of morsitans tsetse flies with particular reference to G. pallidipes (Diptera, Glossinidae). Cytobios 30, 135–152.Google Scholar
Swofford, D.L. & Selander, R.B. (1981) BIOSYS-1: a FORTRAN program for the comprehensive analysis of electrophoretic data in population genetics and systematics. Journal of Heredity 72, 281–283.Google Scholar
Torr, S.J., Parker, A.G. & Leigh-Browne, G. (1988) The responses of Glossina pallidipes Austen (Diptera: Glossinidae) to odour-baited traps and targets in Somalia. Bulletin of Entomological Research 79, 99–108.CrossRefGoogle Scholar
Van Etten, J. (1982) Enzyme polymorphisms in populations of the tsetse fly Glossina pallidipes in Kenya. Entomologia Experimentalis et Applicata 31, 197–201.Google Scholar
Weir, B.S. & Cockerham, C.C. (1984) Estimating F-statistics for the analysis of population structure. Evolution 38, 1358–1370.Google ScholarPubMed
Workman, P.L. & Niswander, J.D. (1970) Population studies on southwestern Indian tribes. 2. Local genetic differentiation in the Papago. American Journal of Human Genetics 22, 24–49.Google Scholar
Wright, S. (1969) Evolution and the genetics of populations, Vol. 2. The theory of gene frequencies. Chicago, University of Chicago Press.Google Scholar
Wright, S. (1978) Evolution and the genetics of populations, Vol. 4. Chicago, University of Chicago Press.Google Scholar