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The absorption and storage of insecticide by the in utero larva of the tsetse fly Glossina Pallidipes Aust

Published online by Cambridge University Press:  10 July 2009

N. S. Irving
N. S. Irving
Affiliation:
Tropical Pesticides Research Institute, Arusha, Tanzania
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Extract

The increase in tolerance to topically applied chlorinated hydrocarbon insecticides shown by pregnant female tsetse flies was investigated in Glossina pallidipes. Solvent extracts of male and pregnant female flies, traeted with DDT and endosulfan labelled with carbon-14, were analysed by thin-layer chromatography and autoradiography of the developed chromatograms. No metabolites of these compounds were detected in the internal and faecal extracts. It was considered, therefore, that detoxication of absorbed insecticide was not the machanism responsible for the lower susceptibility to insecticide in the pregnant female fly. It was shown, however, by the above technique, that insecticide was absorbed by the in utero larva.

Measurement of labelled DDT absorbed by the larva was carried out by liquid scintillation counting. The results indicated that this insecticide was slowly taken up by the larva, in an amount increasing with time, and it was suggested that inert storage of toxicant in the larva is a pregnant female. This would be in addition to the effects of increase in weight and other possible physiological factors.

Type
Research Paper
Information
Bulletin of Entomological Research , Volume 58 , Issue 2 , November 1968 , pp. 221 - 226
Copyright
Copyright © Cambridge University Press 1968

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References

Burnett, G. F. (1962). The susceptibility of tsetse flies to topical applications of insecticides. III. The effects of age and pregnancy on the susceptibility of adults of Glossina morsitans Westw.—Bull. ent. Res. 53 pp. 337–345.Google Scholar
Burnett, G. F. (1963). The susceptibility of tsetse flies to topical applications of insecticides. V. Young adults of Glossina morsitans Westw. and some substituted N-methyl carbamates.—Bull. ent. Res. 53 pp. 747–752.CrossRefGoogle Scholar
Hoskins, W. M. & Gordon, H. T. (1956). Arthropod resistance to chemicals.—A. Rev. Ent. 1 pp. 89–122.CrossRefGoogle Scholar
Hoskins, W. M. Miskus, R. & Eldefrawi, M. E. (1958). The biochemistry of DDT resistance in insects—seminar on the susceptibility of insects to insecticides.—Rep. pan-Am. Hlth Org. Panama 1958.Google Scholar
Leroux, E. J. & Morrison, F. O. (1954). The absorption, distribution, and site of action of DDT in DDT-resistant and DDT-susceptible house flies using carbon-14 labelled DDT.—J. econ. Ent. 47 pp. 1058–1066.CrossRefGoogle Scholar
Litchfield, J. T. & Wilcoxon, F. (1949). A simplified method of evaluating dose effect experiments.—J. Pharmac. exp. Ther. 96 pp. 99–113.Google ScholarPubMed
Sternburg, J. & Kearns, C. W. (1950). Degradation of DDT by resistant and susceptible strains of house flies.—Ann. ent. Soc. Am. 43 pp. 444–458.CrossRefGoogle Scholar
Tahori, A. S. & Hoskins, W. M. (1953). The absorption, distribution, and metabolism of DDT in DDT-resistant houseflies.—J. econ. Ent. 46 pp. 302–306.CrossRefGoogle Scholar
Walker, K. C. & Beroza, M. (1963). Thin-layer chromatography for insecticide analysis.—J. Ass. off. agric. Chem. 46 pp. 250–261.Google Scholar