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The small heat shock protein Hsp20.8 imparts tolerance to high temperatures in the leafminer fly, Liriomyza trifolii (Diptera: Agtomyzidae)

Published online by Cambridge University Press:  13 March 2024

Yue Zhang
Affiliation:
College of Plant Protection, Yangzhou University, Yangzhou, China
Ya-Wen Chang*
Affiliation:
College of Plant Protection, Yangzhou University, Yangzhou, China
Yu-Cheng Wang
Affiliation:
College of Plant Protection, Yangzhou University, Yangzhou, China
Yu-Qing Yan
Affiliation:
College of Plant Protection, Yangzhou University, Yangzhou, China
Yu-Zhou Du*
Affiliation:
College of Plant Protection, Yangzhou University, Yangzhou, China Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education, Yangzhou University, Yangzhou, China
*
Corresponding author: Ya-Wen Chang; Email: changyawen@yzu.edu.cn
Corresponding author: Ya-Wen Chang; Email: changyawen@yzu.edu.cn

Abstract

As an environmental factor, temperature impacts the distribution of species and influences interspecific competition. The molecular chaperones encoded by small heat shock proteins (sHsps) are essential for rapid, appropriate responses to environmental stress. This study focuses on Hsp20.8, which encodes a temperature-responsive sHsp in Liriomyza trifolii, an insect pest that infests both agricultural and ornamental crops. Hsp20.8 expression was highest at 39℃ in L. trifolii pupae and adults, and expression levels were greater in pupae than in adults. Recombinant Hsp20.8 was expressed in Escherichia coli and conferred a higher survival rate than the empty vector to bacterial cells exposed to heat stress. RNA interference experiments were conducted using L. trifolii adults and prepupae and the knockdown of Hsp20.8 expression increased mortality in L. trifolii during heat stress. The results expand our understanding of sHsp function in Liriomyza spp. and the ongoing adaptation of this pest to climate change. In addition, this study is also important for predicting the distribution of invasive species and proposing new prevention and control strategies based on temperature adaptation.

Type
Research Paper
Copyright
Copyright © The Author(s), 2024. Published by Cambridge University Press

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