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Mechanistic effects of microwave radiation on pupal emergence in the leafminer fly, Liriomyza trifolii

Published online by Cambridge University Press:  12 December 2022

Ya-Wen Chang
Affiliation:
School of Plant Protection & Institute of Applied Entomology, Yangzhou University, Yangzhou, China
Yue Zhang
Affiliation:
School of Plant Protection & Institute of Applied Entomology, Yangzhou University, Yangzhou, China
Yu-Qing Yan
Affiliation:
School of Plant Protection & Institute of Applied Entomology, Yangzhou University, Yangzhou, China
Yu-Cheng Wang
Affiliation:
School of Plant Protection & Institute of Applied Entomology, Yangzhou University, Yangzhou, China
Cheng-Dong Wu
Affiliation:
Pukou Agricultural Technology Extension Center of Nanjing City, Pukou, China
Jie Hu
Affiliation:
Plant Protection and Quarantine Station of Jiangsu Province, Nanjing, China
Yu-Zhou Du*
Affiliation:
School of Plant Protection & Institute of Applied Entomology, Yangzhou University, Yangzhou, China Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education, Yangzhou University, Yangzhou, China
*
Author for correspondence: Yu-Zhou Du, Email: yzdu@yzu.edu.cn

Abstract

Liriomyza trifolii is a significant pest of vegetable and ornamental crops across the globe. Microwave radiation has been used for controlling pests in stored products; however, there are few reports on the use of microwaves for eradicating agricultural pests such as L. trifolii, and its effects on pests at the molecular level is unclear. In this study, we show that microwave radiation inhibited the emergence of L. trifolii pupae. Transcriptomic studies of L. trifolii indicated significant enrichment of differentially expressed genes (DEGs) in ‘post-translational modification, protein turnover, chaperones’, ‘sensory perception of pain/transcription repressor complex/zinc ion binding’ and ‘insulin signaling pathway’ when analyzed with the Clusters of Orthologous Groups, Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes databases, respectively. The top DEGs were related to reproduction, immunity and development and were significantly expressed after microwave radiation. Interestingly, there was no significant difference in the expression of genes encoding heat shock proteins or antioxidant enzymes in L. trifolii treated with microwave radiation as compared to the untreated control. The expression of DEGs encoding cuticular protein and protein takeout were silenced by RNA interference, and the results showed that knockdown of these two DEGs reduced the survival of L. trifolii exposed to microwave radiation. The results of this study help elucidate the molecular response of L. trifolii exposed to microwave radiation and provide novel ideas for control.

Type
Research Paper
Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press

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